A method for identification of dermatophyte antigens in situ by an immunoperoxidase technique in light and electron microscopy

1981 ◽  
Vol 6 (3) ◽  
pp. 311-316 ◽  
Author(s):  
C.A. HOLDEN ◽  
R.J. HAY ◽  
D.M. MACDONALD
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Immunoperoxidase Technique

mRNA localization by Electron microscopic in situ hybridization

1991 ◽  
Vol 49 ◽  
pp. 430-431
Author(s):  
J. A. Pollock ◽  
M. Martone ◽  
T. Deerinck ◽  
M. H. Ellisman
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Nucleic Acids ◽  
Recombinant Dna ◽  
Light And Electron Microscopy ◽  
Electron Microscopic ◽  
High Voltage Electron Microscopy ◽  
Functional Sites ◽  
Voltage Electron

Localization of specific proteins in cells by both light and electron microscopy has been facilitate by the availability of antibodies that recognize unique features of these proteins. High resolution localization studies conducted over the last 25 years have allowed biologists to study the synthesis, translocation and ultimate functional sites for many important classes of proteins. Recently, recombinant DNA techniques in molecular biology have allowed the production of specific probes for localization of nucleic acids by “in situ” hybridization. The availability of these probes potentially opens a new set of questions to experimental investigation regarding the subcellular distribution of specific DNA's and RNA's. Nucleic acids have a much lower “copy number” per cell than a typical protein, ranging from one copy to perhaps several thousand. Therefore, sensitive, high resolution techniques are required. There are several reasons why Intermediate Voltage Electron Microscopy (IVEM) and High Voltage Electron Microscopy (HVEM) are most useful for localization of nucleic acids in situ.

scholarly journals Localization of ‘Candidatus Liberibacter solanacearum’ and Evidence for Surface Appendages in the Potato Psyllid Vector

2016 ◽  
Vol 106 (2) ◽  
pp. 142-154 ◽  
Author(s):  
J. M. Cicero ◽  
T. W. Fisher ◽  
J. K. Brown
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Fluorescence Labeling ◽  
Potato Psyllid ◽  
Bactericera Cockerelli ◽  
Visual Identification ◽  
Transmission Electron ◽  
Candidatus Liberibacter ◽  
Identification Of Bacteria

The potato psyllid Bactericera cockerelli is implicated as the vector of the causal agent of zebra chip of potato and vein-greening of tomato diseases. Until now, visual identification of bacteria in the genus ‘Candidatus Liberibacter’ has relied on direct imaging by light and electron microscopy without labeling, or with whole-organ fluorescence labeling only. In this study, aldehyde fixative followed by a coagulant fixative, was used to process adult psyllids for transmission electron microscopy (TEM) colloidal gold in situ hybridization experiments. Results indicated that ‘Ca. Liberibacter solanacearum’ (CLso)-specific DNA probes annealed to a bacterium that formed extensive, monocultural biofilms on gut, salivary gland, and oral region tissues, confirming that it is one morphotype of potentially others, that is rod-shaped, approximately 2.5 µm in diameter and of variable length, and has a rough, granular cytosol. In addition, CLso, prepared from shredded midguts, and negatively stained for TEM, possessed pili- and flagella-like surface appendages. Genes implicating coding capacity for both types of surface structures are encoded in the CLso genome sequence. Neither type was seen for CLso associated with biofilms within or on digestive organs, suggesting that their production is stimulated only in certain environments, putatively, in the gut during adhesion leading to multiplication, and in hemolymph to afford systemic invasion.

Distribution of meprin in kidneys from mice with high- and low-meprin activity

1987 ◽  
Vol 253 (4) ◽  
pp. C535-C540 ◽  
Author(s):  
S. S. Craig ◽  
J. F. Reckelhoff ◽  
J. S. Bond
Keyword(s):  
Electron Microscopy ◽  
Brush Border ◽  
Inbred Mouse ◽  
Light And Electron Microscopy ◽  
Mouse Strains ◽  
Immunoperoxidase Technique ◽  
Inbred Mouse Strains ◽  
Western Blots ◽  
Reactive Material ◽  
Low Activity

An inherited deficiency of a metalloendopeptidase (meprin) activity occurs in kidneys of many inbred mouse strains. To clarify whether meprin protein is present in low-activity strains and determine the distribution of meprin in kidneys of mice with high- and low-meprin activities, kidney slices were stained through the use of the indirect immunoperoxidase technique and examined by light and electron microscopy. Light microscopy at high dilutions of anti-meprin IgG confirmed the brush border localization of meprin in high-meprin activity strains and revealed no detectable cross-reactive material in low-meprin activity strains. However, light and electron microscopy studies that use lower dilutions of anti-meprin immunoglobulin G (IgG) revealed cross-reactivity in low-activity strains, also at the luminal surface of the proximal tubules. Studies at lower magnifications indicated that meprin is primarily associated with the juxtamedullary region of the kidney in both high- and low-activity strains. Western blots of urinary proteins showed significant amounts of meprin-like proteins, but only in the urine of mice with high-meprin activity. The low activity of meprin in some inbred mouse strains is not associated with the presence of the protein in compartments of kidney cells other than the brush border or with secretion of the protein into the urine.

A Simple Procedure for in Situ Immunolabeling, Embedding and Sectioning of Layers of Cultured Endothelial and Smooth Muscle Cells for both Light and Electron Microscopy

1997 ◽  
Vol 72 (1) ◽  
pp. 45-48
Author(s):  
Andre B. Mulder ◽  
Nel R. Blom ◽  
Jan van der Meer ◽  
M. Ruud Halie ◽  
Jan W. Smit
Keyword(s):  
Electron Microscopy ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Simple Procedure ◽  
Light And Electron Microscopy ◽  
Muscle Cells

scholarly journals Using integrated correlative cryo-light and electron microscopy to directly observe syntaphilin-immobilized neuronal mitochondria in situ

2017 ◽  
Vol 3 (1-3) ◽  
pp. 8-16 ◽  
Author(s):  
Shengliu Wang ◽  
Shuoguo Li ◽  
Gang Ji ◽  
Xiaojun Huang ◽  
Fei Sun
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy

COMPARATIVE EFFECT OF INSULIN ON EXPLANTED ADRENAL MEDULLARY TISSUE AND RAT ADRENAL MEDULLA IN SITU

1975 ◽  
Vol 64 (2) ◽  
pp. 323-NP ◽  
Author(s):  
R. S. PIEZZI ◽  
L. A. POHORECKY ◽  
J. C. CAVICCHIA ◽  
J. P. GALLEANO
Keyword(s):  
Electron Microscopy ◽  
Anterior Chamber ◽  
Adrenal Medulla ◽  
Chromaffin Cells ◽  
Insulin Treatment ◽  
Light And Electron Microscopy ◽  
Comparative Effect ◽  
Medullary Tissue ◽  
Releasing Effect

SUMMARY The adrenal medulla and explanted medullary cells in the anterior chamber of the eye were examined 3 h after the administration of insulin to rats. No alterations were seen in the explanted cells. In the adrenal medulla, light and electron microscopy indicated depletion of adrenaline cells; noradrenaline cells were not affected. Levels of catecholamines were three times higher in the explanted tissue after insulin treatment; in the medulla in situ they declined by 70%. These results confirm that insulin has no direct releasing effect on chromaffin cells.

In situ studies on the cytochemistry and ultrastructure of a symbiotic marine dinoflagellate

1968 ◽  
Vol 48 (2) ◽  
pp. 349-366 ◽  
Author(s):  
D. L. Taylor
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Light And Electron Microscopy ◽  
Structural Morphology ◽  
Invertebrate Species ◽  
Algal Symbionts ◽  
Anemonia Sulcata ◽  
Mode Of Life ◽  
Structural Adaptations

The intertidal actinian Anemonia sulcata is known to harbour yellow-brown algal symbionts which are similar in appearance to the zooxanthellae of hermatypic, or reef-building, corals and a number of other invertebrate species. The cytochemistry and structural morphology of the zooxanthella has been studied by light and electron microscopy, to help define it taxonomically and to reveal something about its relations with the actinian. These investigations confirm that it is a dinoflagellate and have revealed several structural adaptations which are formed as a result of the peculiar mode of life adopted by this alga. Of significance is the fine structure of the periplast, which may have a considerable bearing upon the type of relationship which can exist between the host and its symbiont. These findings are discussed in terms of other known instances of algal-invertebrate symbiosis.

Techniques Involved in the Acquisition of Serial Sections of the Atrioventricular Node for Light and Electron Microscopy

1968 ◽  
Vol 26 ◽  
pp. 226-227
Author(s):  
Sheila S. Emmett ◽  
J. C. Thaemert
Keyword(s):  
Electron Microscopy ◽  
Osmium Tetroxide ◽  
Light And Electron Microscopy ◽  
Atrioventricular Node ◽  
Electron Microscopic Level ◽  
Serial Sections ◽  
Electron Microscopic ◽  
Trapezoidal Shape ◽  
Old Mice

The acquisition of serial sections of the atrioventricular node for light and electron microscopy is a formidable task. Ordinary techniques are not adequate if the best possible results are to be achieved at the electron microscopic level. The techniques outlined below have proven to be valuable in locating and determining the position of the AV node.Whole hearts of 2-week old mice were fixed, in situ, by perfusion with 1% phosphate-buffered osmium tetroxide. The hearts were removed from the animals, sectioned transversely into 3 slices approximately equal in thickness, dehydrated in graded concentrations of ethanol and embedded in Epon 812. The block faces were trimmed to a trapezoidal shape ranging in size from 0.75 x 1 mm to 4 x 5 mm. Serial sections approximately 2 microns in thickness were cut with glass knives on a Porter-Blum MT-2 Ultramicrotome. While floating on a drop of water on the knife, each section was stretched with 1 drop of a 1:1, xylene in chloroform mixture applied directly to the section. The sections were picked up individually with a brush, transferred to a glass slide and oven dried for several hours prior to staining.

In situ flat embedding of monolayers and cell relocation in the acrylic resin LR White for comparative light and electron microscopy studies

1994 ◽  
Vol 26 (12) ◽  
pp. 934-938 ◽  
Author(s):  
Marianne Steiner ◽  
Christian Schöfer ◽  
Wilhelm Mosgoeller
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Acrylic Resin ◽  
Lr White ◽  
Flat Embedding
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