A Simple Procedure for in Situ Immunolabeling, Embedding and Sectioning of Layers of Cultured Endothelial and Smooth Muscle Cells for both Light and Electron Microscopy

1997 ◽  
Vol 72 (1) ◽  
pp. 45-48
Author(s):  
Andre B. Mulder ◽  
Nel R. Blom ◽  
Jan van der Meer ◽  
M. Ruud Halie ◽  
Jan W. Smit
Keyword(s):  
Electron Microscopy ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Simple Procedure ◽  
Light And Electron Microscopy ◽  
Muscle Cells

Presence of smooth-muscle cells in the subdural neomembrane

1981 ◽  
Vol 54 (5) ◽  
pp. 646-651 ◽  
Author(s):  
Nobuyuki Kawano ◽  
Kinuko Suzuki
Keyword(s):  
Electron Microscopy ◽  
Smooth Muscle ◽  
Light Microscopy ◽  
Smooth Muscle Cells ◽  
Subdural Hematoma ◽  
Light And Electron Microscopy ◽  
Chronic Subdural Hematoma ◽  
Muscle Cells ◽  
Content Type ◽  
Fine Print

✓ The authors encountered a case of chronic subdural hematoma of which the subdural neomembrane (SN) showed numerous spindle-shaped cells identified as smooth-muscle cells (SMC's) by electron microscopy. On reexamination of 214 cases from the files, SMC's were found with light microscopy in seven cases. In these cases, the SN was well organized (collagenized). In three additional cases examined with both light and electron microscopy, SMC's were not apparent with light microscopy. However, in all cases, cells with ultrastructural features of both fibroblasts and SMC's were observed. Well formed SMC's were found in two additional cases of well organized membrane. Based on these observations, it is concluded that the presence of SMC's in the SN is not a rare phenomenon. The possible origin of SMC's in the SN and their pathological significance to the organizing process of chronic subdural hematoma are discussed.

Preparation of recombinant human-like collagen/fibroin scaffold and its promoting effect on vascular cells biocompatibility

2018 ◽  
Vol 33 (4) ◽  
pp. 416-425 ◽  
Author(s):  
Jia Yan ◽  
Kun Hu ◽  
YongHao Xiao ◽  
Fan Zhang ◽  
Lu Han ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Laser Scanning ◽  
Muscle Cells ◽  
Laser Scanning Confocal Microscopy ◽  
Scanning Confocal Microscopy ◽  
Scanning Electron

A novel recombinant human-like collagen/fibroin scaffold has been prepared previously, which has high porosity, controllable pore size, and much better mechanical properties than the reported fibroin-based scaffold. In this research, the cell responses of vascular smooth muscle cells to this blend scaffold were examined in vitro. Cell morphology, adherence, and growth in scaffolds were observed by scanning electron microscopy, laser scanning confocal microscopy after staining of the cells with propidium iodide at 1, 3, 5, and 7 days, respectively. A wide range of measurements, including 3-[4,5–dimethylthiazol-2-yl]-2, 5-diphenyl tetrasodium bromide assay, and total intracellular protein content at the end of 7 days culture, were conducted. An increase of viability and protein content of vascular smooth muscle cells cultured in recombinant human-like collagen/fibroin scaffold was found. The laser scanning confocal microscopy and scanning electron microscopy results confirm that the cells readily adhered and proliferation in the blend than in fibroin scaffold, and indicate a better adhesion process. The positive effects were especially significant for vascular smooth muscle cells. The recombinant human-like collagen/fibroin scaffold could be a promising biomaterial for vascular tissue engineering.

Histological maturation of vascular smooth muscle cells in in situ tissue-engineered vasculature

Biomaterials ◽  
2014 ◽  
Vol 35 (11) ◽  
pp. 3589-3595 ◽  
Author(s):  
Noriko Isayama ◽  
Goki Matsumura ◽  
Hideki Sato ◽  
Shojiro Matsuda ◽  
Kenji Yamazaki
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells

Loss and apoptosis of smooth muscle cells in intracranial aneurysms studies with in situ DNA end labeling and antibody against single-stranded DNA

1997 ◽  
Vol 139 (5) ◽  
pp. 469-475 ◽  
Author(s):  
T. Sakaki ◽  
E. Kohmura ◽  
T. Kishiguchi ◽  
T. Yuguchi ◽  
T. Yamashita ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Intracranial Aneurysms ◽  
Muscle Cells ◽  
Single Stranded Dna

scholarly journals FINE STRUCTURE OF SMOOTH MUSCLE CELLS GROWN IN TISSUE CULTURE

1971 ◽  
Vol 49 (1) ◽  
pp. 21-34 ◽  
Author(s):  
Gordon R. Campbell ◽  
Yasuo Uehara ◽  
Gerda Mark ◽  
Geoffrey Burnstock
Keyword(s):  
Electron Microscopy ◽  
Smooth Muscle ◽  
Fine Structure ◽  
Cell Membrane ◽  
Smooth Muscle Cells ◽  
Phase Contrast ◽  
Muscle Cells ◽  
Different Types ◽  
Membrane Infoldings

The fine structure of smooth muscle cells of the embryo chicken gizzard cultured in monolayer was studied by phase-contrast optics and electron microscopy. The smooth muscle cells were irregular in shape, but tended to be elongate. The nucleus usually contained prominent nucleoli and was large in relation to the cell body. When fixed with glutaraldehyde, three different types of filaments were noted in the cytoplasm: thick (150–250 A in diameter) and thin (30–80 A in diameter) myofilaments, many of which were arranged in small bundles throughout the cytoplasm and which were usually associated with dark bodies; and filaments with a diameter of 80–110 A which were randomly orientated and are not regarded as myofilaments. Some of the aggregated ribosomes were helically arranged. Mitochondria, Golgi apparatus, and dilated rough endoplasmic reticulum were prominent. In contrast to in vivo muscle cells, micropinocytotic vesicles along the cell membrane were rare and dense areas were usually confined to cell membrane infoldings. These cells are compared to in vivo embryonic smooth muscle and adult muscle after treatment with estrogen. Monolayers of cultured smooth muscle will be of particular value in relating ultrastructural features to functional observations on the same cells.

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