Intracellular T lymphocyte cytokine profiles in the aqueous humour of patients with uveitis and correlation with clinical phenotype

Abstract Background: To investigate aqueous humour (AH) cytokine profiles in eyes with Coats disease and analyze the association between cytokine concentrations and the severity of the disease. Methods: The study included 36 patients (36 eyes) with Coats disease and 15 control patients (15 eyes) with congenital cataract. AH samples were obtained preoperatively and the concentrations of 22 different cytokines were measured through Cytometric Bead Array technology. Clinical characteristics of Coats disease, including the extent of retinal exudation and exudative retinal detachment (ERD), were recorded for analysis. Results: The concentrations of 8 cytokines (VEGF, IL-6, IL-8, MCP-1, MIP-1α, IP-10, VCAM-1 and ICAM-1) were significantly higher in the Coats disease group than in the control group (all P < 0.002). Except for VCAM-1 and ICAM-1, the concentration of the other cytokines listed above showed a significant increase from stage 2 to stage 3 (all P < 0.05). Meanwhile, the concentrations of VEGF, IL-8, MCP-1 and MIP-1α showed a significant and positive association with the extent of retinal exudation and ERD (all r > 0.4, P < 0.05). Among these, IL-8 showed a strong association with the extent of retinal exudation and ERD (all r > 0.7, P < 0.001). The concentrations of IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-10, IL-12, Fractalkine, RANTES, G-CSF and GM-CSF were very low in both groups. Conclusions: Various cytokines in the AH, including elevated VEGF, IL-6, IL-8, MCP-1, MIP-1α, IP-10, VCAM-1 and ICAM-1, may be involved in the pathogenesis and progression of Coats disease. Increasing severity of Coats disease is significantly associated with the AH concentrations of VEGF, IL-8, MCP-1 and MIP-1α. Further clinical treatment aimed to reduce vascular leakage and antagonize neovascularization and inflammation may be useful in preventing the progression of Coats disease.

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Comparative analysis of cytokine profiles of glaucomatous tears and aqueous humour reveals potential biomarkers for trabeculectomy complications

FEBS Open Bio ◽

10.1002/2211-5463.12637 ◽

2019 ◽

Vol 9 (5) ◽

pp. 1020-1028 ◽

Cited By ~ 3

Author(s):

Éva Csősz ◽

Eszter Deák ◽

Noémi Tóth ◽

Carlo Enrico Traverso ◽

Adrienne Csutak ◽

...

Keyword(s):

Comparative Analysis ◽

Aqueous Humour ◽

Cytokine Profiles ◽

Potential Biomarkers

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Aqueous humour cytokine profiles after Descemet’s membrane endothelial keratoplasty

Scientific Reports ◽

10.1038/s41598-021-96566-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Takahiko Hayashi ◽

Hidenori Takahashi ◽

Satoru Inoda ◽

Toshiki Shimizu ◽

Akira Kobayashi ◽

...

Keyword(s):

Inflammatory Cytokines ◽

Aqueous Humour ◽

Control Group ◽

Bullous Keratopathy ◽

Endothelial Keratoplasty ◽

Cytokine Profiles ◽

Descemet’S Membrane Endothelial Keratoplasty ◽

Descemet's Membrane ◽

Descemet’S Membrane ◽

Pro Inflammatory Cytokines

AbstractThe aim of this study was to compare aqueous humour (AqH) cytokine profiles before and after Descemet’s membrane endothelial keratoplasty (DMEK) in eyes with bullous keratopathy (BK) and age-matched normal eyes. A total of 49 participants (mean age 75.0 ± 13.5 years) were divided into three groups: (1) BK patients scheduled for DMEK (BK group); (2) patients after DMEK (DMEK group; average postoperative time 1,018 ± 460 days); and (3) control participants without corneal endothelial disease scheduled for cataract surgery (control group). AqH (0.2 mL) was collected from all participants, and the levels of various AqH cytokines were simultaneously measured using a multiplex bead immunoassay. The DMEK group exhibited significantly lower concentrations of several pro-inflammatory cytokines, such as IL-1β, IL-5, IL-6, IL-10, and IL-8, and granulocyte colony stimulating factor than the BK group. In addition, the levels of IL-1β and IL-5 were significantly lower in the DMEK group than in the control group. The AqH levels of several pro-inflammatory cytokines were significantly reduced after DMEK, indicating that regeneration of the endothelial pump owing to the transplantation of a healthy endothelium might have a positive effect on anterior chamber inflammation.

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Cytokine profiles of Porphyromonas gingivalis-reactive T lymphocyte lines and clones derived from P. gingivalis-infected subjects

Oral Diseases ◽

10.1111/j.1601-0825.1995.tb00176.x ◽

2008 ◽

Vol 1 (3) ◽

pp. 139-146 ◽

Cited By ~ 27

Author(s):

E Gemmell ◽

M Kjeldsen ◽

K Yamazaki ◽

T Nakajima ◽

MJ Aldred ◽

...

Keyword(s):

Porphyromonas Gingivalis ◽

T Lymphocyte ◽

Cytokine Profiles

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Mitochondrial Superoxide Signaling Contributes to Norepinephrine-Mediated T-Lymphocyte Cytokine Profiles

PLoS ONE ◽

10.1371/journal.pone.0164609 ◽

2016 ◽

Vol 11 (10) ◽

pp. e0164609 ◽

Cited By ~ 13

Author(s):

Adam J. Case ◽

Colton T. Roessner ◽

Jun Tian ◽

Matthew C. Zimmerman

Keyword(s):

T Lymphocyte ◽

Cytokine Profiles ◽

Mitochondrial Superoxide

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Altered T lymphocyte subtypes and cytokine profiles in follicular fluid associated with diminished ovary reserve

American Journal of Reproductive Immunology ◽

10.1111/aji.13522 ◽

2022 ◽

Author(s):

Nannan Zhao ◽

Ce Zhang ◽

Jie Ding ◽

Huihua Wu ◽

Wei Cheng ◽

...

Keyword(s):

T Lymphocyte ◽

Follicular Fluid ◽

Cytokine Profiles ◽

Ovary Reserve

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T-lymphocyte cytokine profiles in Compositae airborne dermatitis

British Journal of Dermatology ◽

10.1046/j.1365-2133.1999.03108.x ◽

1999 ◽

Vol 141 (4) ◽

pp. 689-693 ◽

Cited By ~ 13

Author(s):

Stingeni ◽

Agea ◽

Lisi ◽

Spinozzi

Keyword(s):

T Lymphocyte ◽

Cytokine Profiles ◽

Airborne Dermatitis

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A 3-Week Dynamic Differences of Immunological Parameters in Severe and Non-severe COVID-19

10.21203/rs.3.rs-36753/v1 ◽

2020 ◽

Author(s):

Qiang Li ◽

Wei Xu ◽

Weixia Li ◽

Chenglu Huang ◽

Liang Chen

Keyword(s):

T Lymphocyte ◽

Lymphocyte Subsets ◽

Immunological Parameters ◽

T Lymphocyte Subsets ◽

Cytokine Profiles ◽

Tnf Α ◽

Severe Group ◽

Ifn Γ ◽

Low Levels

Abstract Objective We aimed to compare the dynamic differences of immunological parameters in severe and non-severe COVID-19. Methods In this study, the cytokine profiles and lymphocyte subsets of 70 patients (31 severe COVID-19 and 39 non-severe COVID-19) were longitudinally analyzed. Results Compared with non-severe cases, severe cases had higher age (64 vs 36 years, p<0.001), more common comorbidities (74.2% vs 15.4%, p<0.001), and more frequently lymphopenia (0.7 vs 1.6×109/L, p<0.001). Severe cases had markedly higher levels of IL-6, IL-8, and IL-10 than non-severe cases from baseline to 3 weeks after admission (p<0.001). No significant differences were observed in the levels of IL-1β, IL-2, IL-4, IL-5, IL-12P70, IL-17, TNF-α, IFN-α, and IFN-γ between the two groups during the follow-up (p > 0.05). The absolute numbers of CD3+, CD4+, CD8+, and CD45+ T cells were markedly lower in severe cases compared with that in non-severe cases from baseline to 3 weeks after admission (p<0.001). The decrease of T lymphocyte subsets reached its peak from day 1 to 3 after admission, and gradually increased from day 4 to 21 in the non-severe group; however, reached its peak from day 4 to 7 after admission, and sustained at a low levels in the severe group. Conclusion The dynamic changes of cytokine profiles and T lymphocyte subsets are related with the disease severity of patients with COVID-19.

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Aqueous humour cytokine profiles in eyes with Coats disease and the association with the severity of the disease

10.21203/rs.2.24719/v2 ◽

2020 ◽

Author(s):

Tingyi Liang ◽

Yu Xu ◽

Xiuyu Zhu ◽

Xiang Zhang ◽

Jing Li ◽

...

Keyword(s):

Aqueous Humour ◽

Strong Association ◽

Positive Association ◽

Control Group ◽

Exudative Retinal Detachment ◽

Gm Csf ◽

Cytokine Profiles ◽

Array Technology ◽

Coats Disease ◽

Severity Of The Disease

Abstract Background: To investigate aqueous humour (AH) cytokine profiles in eyes with Coats disease and analyze the association between cytokine concentrations and the severity of the disease. Methods: The study included 36 patients (36 eyes) with Coats disease and 15 control patients (15 eyes) with congenital cataract. AH samples were obtained preoperatively and the concentrations of 22 different cytokines were measured through Cytometric Bead Array technology. Clinical characteristics of Coats disease, including the extent of retinal exudation and exudative retinal detachment (ERD), were recorded for analysis. Results: The concentrations of 8 cytokines (VEGF, IL-6, IL-8, MCP-1, MIP-1α, IP-10, VCAM-1 and ICAM-1) were significantly higher in the Coats disease group than in the control group (all P < 0.002). Except for VCAM-1 and ICAM-1, the concentration of the other cytokines listed above showed a significant increase from stage 2 to stage 3 (all P < 0.05). Meanwhile, the concentrations of VEGF, IL-8, MCP-1 and MIP-1α showed a significant and positive association with the extent of retinal exudation and ERD (all r > 0.4, P < 0.05). Among these, IL-8 showed a strong association with the extent of retinal exudation and ERD (all r > 0.7, P < 0.001). The concentrations of IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-10, IL-12, Fractalkine, RANTES, G-CSF and GM-CSF were very low in both groups. Conclusions: Various cytokines in the AH, including elevated VEGF, IL-6, IL-8, MCP-1, MIP-1α, IP-10, VCAM-1 and ICAM-1, may be involved in the pathogenesis and progression of Coats disease. Increasing severity of Coats disease is significantly associated with the AH concentrations of VEGF, IL-8, MCP-1 and MIP-1α. Further clinical treatment aimed to reduce vascular leakage and antagonize neovascularization and inflammation may be useful in preventing the progression of Coats disease.

Download Full-text