scholarly journals High density of intraepithelial γδ lymphocytes and deposits of immunoglobulin (Ig)M anti-tissue transglutaminase antibodies in the jejunum of coeliac patients with IgA deficiency

2009 ◽  
Vol 160 (2) ◽  
pp. 199-206 ◽  
Author(s):  
M. Borrelli ◽  
M. Maglio ◽  
M. Agnese ◽  
F. Paparo ◽  
S. Gentile ◽  
...  
Keyword(s):  
Tissue Transglutaminase ◽  
Iga Deficiency ◽  
High Density ◽  
Tissue Transglutaminase Antibodies ◽  
Γδ Lymphocytes

A comparative study of tissue transglutaminase antibodies and endomysium antibody immunofluorescence in routine clinical laboratory practice

2003 ◽  
Vol 40 (4) ◽  
pp. 411-416 ◽  
Author(s):  
David Sinclair ◽  
Callum B Pearce ◽  
Michael S. L. Saas ◽  
David Poller
Keyword(s):  
Guinea Pig ◽  
Coeliac Disease ◽  
Clinical Laboratory ◽  
Tissue Transglutaminase ◽  
Iga Deficiency ◽  
Complete Agreement ◽  
Tissue Transglutaminase Antibodies ◽  
Routine Clinical Laboratory ◽  
Endomysium Antibodies ◽  
Human Source

Background: The demand for screening for coeliac disease has grown rapidly over the last few years. Laboratories depending on immunofluorescence assays are faced with an increasing workload using a labour-intensive test, and an alternative to this test has been sought. This study compares tissue transglutaminase (TTG) and endomysium antibodies (EMA) in a routine clinical laboratory situation. Methods: An immunofluorescence IgA EMA test was compared with a guinea pig TTG antibody ELISA for 816 unselected requests for gut antibody screening. Discrepant results were investigated more fully using a variety of human source TTG antigen kits. Results: Guinea pig TTG ELISA and EMA assays showed agreement for 93·6% of samples. Four samples were misclassified and 48 samples gave false positive TTG results. Study of 46 EMA samples (this group included 39 of the 'discrepant' negative EMA/positive guinea pig TTG group) using three different human purified and/or recombinant TTG sources showed that 42 patients had no TTG antibodies using human sources, three were misclassified and one patient had negative EMA and positive TTG results that could not be readily explained. Further study of 32 EMA positive samples showed almost complete agreement between the human source TTG kits. Conclusions: We can recommend the replacement of EMA with ELISA for TTG antibodies for the routine screening for coeliac disease, but all positive TTG antibodies should still be followed up with IgA EMA and samples should be screened for IgA deficiency.

scholarly journals Immunoglobulin G (IgG) Anti-Tissue Transglutaminase Antibodies Used as Markers for IgA-Deficient Celiac Disease Patients

2005 ◽  
Vol 12 (2) ◽  
pp. 254-258 ◽  
Author(s):  
Ingrid Dahlbom ◽  
Martin Olsson ◽  
Nahal Kazemi Forooz ◽  
Anders G. Sjöholm ◽  
Lennart Truedsson ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Tissue Transglutaminase ◽  
Immunoglobulin A ◽  
Good Alternative ◽  
Iga Deficiency ◽  
Enzyme Linked Immunosorbent Assay ◽  
Screening Methods ◽  
Igg Antibodies ◽  
Negative Results ◽  
Tissue Transglutaminase Antibodies

ABSTRACT The role of immunoglobulin A (IgA) anti-tissue transglutaminase antibodies (IgA-tTG) as predictors of untreated celiac disease (CoD) is well documented, and the presence and levels of these antibodies are most accurately monitored with native or recombinant human antigens. However, IgA-deficient CoD patients are not identified by IgA serology, and conflicting results concerning the diagnostic validity of IgG antibodies against gliadin (IgG-AGA), endomysium (IgG-EmA), and tTG (IgG-tTG) have been reported. The aim of the present study was to evaluate the utility of IgG-tTG for the detection of CoD in IgA-deficient patients. Samples from 115 IgA-deficient and 200 IgA-sufficient subjects were collected and tested for the presence of IgA and IgG antibodies against tTG, EmA, and AGA. Antibodies against tTG were measured by an enzyme-linked immunosorbent assay based on recombinant human tTG, and antibodies against EmA were determined by immunofluorescence. The values for IgG-tTG showed a higher correlation (correlation coefficient [r] = 0.91) with those for IgG-EmA for the IgA-deficient subjects than for the IgA-sufficient subjects (r = 0.88). The overall concordance of the positive and negative results between IgG-tTG and IgG-EmA was 97%, and the IgG-tTG assay discriminated between IgG-EmA-positive and -negative subjects with IgA deficiency at a rate of 100%. Elevated levels of IgG-tTG and IgG-EmA were measured in 70% of the IgA-sufficient subjects. IgG-tTG detection with recombinant human tTG is a good alternative to IgG-EmA detection, and the addition of IgG-tTG assessment to present screening methods may improve the ability to identify IgA-deficient subjects with CoD.

scholarly journals A rare case of Addison’s disease, hepatitis, thyreoiditis, positive IgG anti-tissue transglutaminase antibodies and partial IgA deficiency

2016 ◽  
Vol 2 ◽  
pp. 217-220
Author(s):  
Marta P. Baleva ◽  
Snejina Mihaylova ◽  
Petja Yankova ◽  
Iliana Atanasova ◽  
Milena Nikolova-Vlahova ◽  
...  
Keyword(s):  
Rare Case ◽  
Tissue Transglutaminase ◽  
Addison’S Disease ◽  
Iga Deficiency ◽  
Addison's Disease ◽  
Tissue Transglutaminase Antibodies

Reliability of anti-tissue transglutaminase antibodies in children with malnutrition

2021 ◽  
Vol 56 (4) ◽  
pp. 378-381
Author(s):  
Necati Balamtekin ◽  
Harun Erdal ◽  
Emira Gençkardeşler ◽  
Melike Arslan
Keyword(s):  
Tissue Transglutaminase ◽  
Tissue Transglutaminase Antibodies

A New Indirect Chemiluminescent Immunoassay to Measure Anti–tissue Transglutaminase Antibodies

2006 ◽  
Vol 43 (5) ◽  
pp. 613-618 ◽  
Author(s):  
Daniela Basso ◽  
Graziella Guariso ◽  
Michela Fasolo ◽  
Marina Pittoni ◽  
Stefania Schiavon ◽  
...  
Keyword(s):  
Tissue Transglutaminase ◽  
Tissue Transglutaminase Antibodies ◽  
Chemiluminescent Immunoassay

scholarly journals Low specificity of anti-tissue transglutaminase antibodies in patients with primary biliary cirrhosis

2006 ◽  
Vol 20 (5) ◽  
pp. 184-189 ◽  
Author(s):  
N. Bizzaro ◽  
M. Tampoia ◽  
D. Villalta ◽  
S. Platzgummer ◽  
M. Liguori ◽  
...  
Keyword(s):  
Primary Biliary Cirrhosis ◽  
Tissue Transglutaminase ◽  
Biliary Cirrhosis ◽  
Tissue Transglutaminase Antibodies

Relationships between Clinical Presentation, Serology, Histology, and Duodenal Deposits of Tissue Transglutaminase Antibodies in Pediatric Celiac Disease

2018 ◽  
Vol 36 (5) ◽  
pp. 369-376 ◽  
Author(s):  
Nurit Loberman-Nachum ◽  
Michael Schvimer ◽  
Camila Avivi ◽  
Iris Barshack ◽  
Avishay Lahad ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Immunohistochemical Staining ◽  
Clinical Presentation ◽  
Tissue Transglutaminase ◽  
Mucosal Damage ◽  
High Serum ◽  
Tissue Transglutaminase Antibodies ◽  
Antibody Levels ◽  
Age Range ◽  
Multiple Symptoms

Background: The clinical, histological, and serological spectrum of celiac disease (CD) vary widely. We aimed to examine relationships between symptoms, serum anti-tissue transglutaminase antibodies (tTG) levels, mucosal damage, and mucosal anti-tTG deposits in pediatric CD. Methods: A retrospective single-center, cohort study of children referred for endoscopy with suspected CD during 2011–2014. We retrieved the clinical data, blindly reviewed duodenal biopsies, and performed immunohistochemical staining for anti-tTG deposits. Patients were classified as monosymptomatic or polysymptomatic. Mucosal anti-tTG deposits were classified according to the location of deposits, dominant intensity, maximal intensity, and percentage of stained area. Results: Of 252 patients with confirmed CD, complete data were available for 100: 37 males in the age range 1.3–16.7 with median 4.0 years. Monosymptomatic patients (n = 54) presented at an older age than polysymptomatic patients (1.3–15.5, median 8.1 vs. 1.3–16.7, median 6.3 years, p = 0.026). Marsh 2–3c was more prevalent in polysymptomatic patients (93 vs. 78%, p = 0.028). The intensity of mucosal anti-tTG deposits correlated with serum anti-tTG levels but not with the clinical presentation. Conclusions: Multiple symptoms and high serum anti-tTG antibody levels correlated with mucosal damage in children with CD. The role of immunohistochemical staining for intestinal anti-tTG mucosal deposits in the diagnosis of borderline CD is not yet established.

Elevated Serum Tissue Transglutaminase Antibodies in Children With Eosinophilic Esophagitis

2017 ◽  
Vol 65 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Anna K. Le Fevre ◽  
Marjorie M. Walker ◽  
Amir Hadjiashrafy ◽  
Rani Bhatia ◽  
Joerg Mattes ◽  
...  
Keyword(s):  
Eosinophilic Esophagitis ◽  
Tissue Transglutaminase ◽  
Elevated Serum ◽  
Tissue Transglutaminase Antibodies

scholarly journals Celiac Disease and Liver Disorders: From Putative Pathogenesis to Clinical Implications

Nutrients ◽  
2018 ◽  
Vol 10 (7) ◽  
pp. 892 ◽  
Author(s):  
Iva Hoffmanová ◽  
Daniel Sánchez ◽  
Ludmila Tučková ◽  
Helena Tlaskalová-Hogenová
Keyword(s):  
Celiac Disease ◽  
Liver Diseases ◽  
Tissue Transglutaminase ◽  
Intestinal Barrier ◽  
Rational Approach ◽  
Liver Disorders ◽  
Tissue Transglutaminase Antibodies ◽  
Elevated Transaminases ◽  
Made In

Immunologically mediated liver diseases belong to the common extraintestinal manifestations of celiac disease. We have reviewed the current literature that addresses the association between celiac disease and liver disorders. We searched relevant articles on MEDLINE/PubMed up to 15 June 2018. The objective of the article is to provide a comprehensive and up-to-date review on the latest hypotheses explaining the pathogenetic relationship between celiac disease and liver injury. Besides the involvement of gut–liver axis, tissue transglutaminase antibodies, and impairment of intestinal barrier, we integrate the latest achievements made in elucidation of the role of gut microbiota in celiac disease and liver disorders, that has not yet been sufficiently discussed in the literature in this context. The further objective is to provide a complete clinical overview on the types of liver diseases frequently found in celiac disease. In conclusion, the review highlights the clinical implication, recommend a rational approach for managing elevated transaminases in celiac patients, and underscore the importance of screening for celiac disease in patients with associated liver disease.

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