Molecular cloning and expression inEscherichia coliof genes encoding pectate lyase and pectin methylesterase activities fromBacteroides thetaiotaomicron

Improved methods are needed to extend the shelf life of strawberry fruits. The objective of this study was to determine the postharvest physiological mechanism of high-CO2 treatment in strawberries. Harvested strawberries were stored at 10 °C after 3 h of exposure to a treatment with 30% CO2 or air. Pectin and gene expression levels related to cell wall degradation were measured to assess the high-CO2 effects on the cell wall and lipid metabolism. Strawberries subjected to high-CO2 treatment presented higher pectin content and firmness and lower decay than those of control fruits. Genes encoding cell wall-degrading enzymes (pectin methylesterase, polygalacturonase, and pectate lyase) were downregulated after high-CO2 treatment. High-CO2 induced the expression of oligogalacturonides, thereby conferring defense against Botrytis cinerea in strawberry fruits, and lowering the decay incidence at seven days after its inoculation. Our findings suggest that high-CO2 treatment can maintain strawberry quality by reducing decay and cell wall degradation.

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Molecular cloning in Escherichia coli of Erwinia chrysanthemi genes encoding multiple forms of pectate lyase.

Journal of Bacteriology ◽

10.1128/jb.161.3.913-920.1985 ◽

1985 ◽

Vol 161 (3) ◽

pp. 913-920 ◽

Cited By ~ 51

Author(s):

A Collmer ◽

C Schoedel ◽

D L Roeder ◽

J L Ried ◽

J F Rissler

Keyword(s):

Escherichia Coli ◽

Molecular Cloning ◽

Pectate Lyase ◽

Erwinia Chrysanthemi ◽

Multiple Forms ◽

Genes Encoding

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Molecular cloning and expression analysis of two genes encoding plant defensins from watermelon (Citrullus lanatus(Thunb.) Matsum. & Nakai)

The Journal of Horticultural Science and Biotechnology ◽

10.1080/14620316.2013.11513033 ◽

2013 ◽

Vol 88 (6) ◽

pp. 743-750

Author(s):

E. A. Zhang ◽

X. P. Yang ◽

J. H. Xu ◽

G. Liu ◽

X. F. Yao ◽

...

Keyword(s):

Molecular Cloning ◽

Expression Analysis ◽

Citrullus Lanatus ◽

Cloning And Expression ◽

Plant Defensins ◽

Genes Encoding

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A Methylotrophic Pathway Participates in Pectin Utilization by Candida boidinii

Applied and Environmental Microbiology ◽

10.1128/aem.66.10.4253-4257.2000 ◽

2000 ◽

Vol 66 (10) ◽

pp. 4253-4257 ◽

Cited By ~ 35

Author(s):

Tomoyuki Nakagawa ◽

Tatsuro Miyaji ◽

Hiroya Yurimoto ◽

Yasuyoshi Sakai ◽

Nobuo Kato ◽

...

Keyword(s):

Methyl Ester ◽

Alcohol Oxidase ◽

Pectate Lyase ◽

Methylotrophic Yeast ◽

Pectin Methylesterase ◽

Candida Boidinii ◽

Methanol Metabolism ◽

Genes Encoding ◽

Dihydroxyacetone Synthase ◽

In Cells

ABSTRACT The methylotrophic yeast Candida boidinii S2 was found to be able to grow on pectin or polygalacturonate as a carbon source. When cells were grown on 1% (wt/vol) pectin, C. boidinii exhibited induced levels of the pectin-depolymerizing enzymes pectin methylesterase (208 mU/mg of protein), pectin lyase (673 mU/mg), pectate lyase (673 mU/mg), and polygalacturonase (3.45 U/mg) and two methanol-metabolizing peroxisomal enzymes, alcohol oxidase (0.26 U/mg) and dihydroxyacetone synthase (94 mU/mg). The numbers of peroxisomes also increased ca. two- to threefold in cells grown on these pectic compounds (3.34 and 2.76 peroxisomes/cell for cells grown on pectin and polygalacturonate, respectively) compared to the numbers in cells grown on glucose (1.29 peroxisomes/cell). The cell density obtained with pectin increased as the degree of methyl esterification of pectic compounds increased, and it decreased in strains from which genes encoding alcohol oxidase and dihydroxyacetone synthase were deleted and in a peroxisome assembly mutant. Our study showed that methanol metabolism and peroxisome assembly play important roles in the degradation of pectin, especially in the utilization of its methyl ester moieties.

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