Enumeration, isolation and identification of diazotrophs from Korean wetland rice varieties grown with long-term application of N and compost and their short-term inoculation effect on rice plants

Being an essential macroelement, sulfur (S) is pivotal for plant growth and development, and acute deficiency in this element leads to yield penalty. Since the last decade, strong evidence has reported the regulatory function of silicon (Si) in mitigating plant nutrient deficiency due to its significant diverse benefits on plant growth. However, the role of Si application in alleviating the negative impact of S deficiency is still obscure. In the present study, an attempt was undertaken to decipher the role of Si application on the metabolism of rice plants under S deficiency. The results showed a distinct transcriptomic and metabolic regulation in rice plants treated with Si under both short and long-term S deficiencies. The expression of Si transporters OsLsi1 and OsLsi2 was reduced under long-term deficiency, and the decrease was more pronounced when Si was provided. The expression of OsLsi6, which is involved in xylem loading of Si to shoots, was decreased under short-term S stress and remained unchanged in response to long-term stress. Moreover, the expression of S transporters OsSULTR tended to decrease by Si supply under short-term S deficiency but not under prolonged S stress. Si supply also reduced the level of almost all the metabolites in shoots of S-deficient plants, while it increased their level in the roots. The levels of stress-responsive hormones ABA, SA, and JA-lle were also decreased in shoots by Si application. Overall, our finding reveals the regulatory role of Si in modulating the metabolic homeostasis under S-deficient condition.

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Comparative analysis of diet-associated responses in two rice planthopper species

10.21203/rs.2.14809/v3 ◽

2020 ◽

Author(s):

Hai-Jian Huang ◽

Jia-Rong Cui ◽

Xiao-Yue Hong

Keyword(s):

Gene Expression ◽

Comparative Analysis ◽

High Throughput Sequencing ◽

Insect Pests ◽

Brown Planthopper ◽

Host Adaptation ◽

Short Term ◽

Expression Change ◽

Rice Plants

Abstract Background: Host adaptation is the major determinant of insect diversification. However, knowledge of different host ranges in very close-related species remains scarce. The brown planthopper (Nilaparvata lugens, BPH) and small brown planthopper (Laodelphax striatellus, SBPH) are the most destructive insect pests belonging to Delphacidae. These two species differ in the host range (SBPH can well colonize on rice and wheat plants, while BPH survive on rice plants only), but the underlying mechanism still remains unknown. High-throughput sequencing technology provides a powerful approach for analyzing the association between gene expression changes and physiological responses of insects. Therefore, the gut transcriptomes were performed to elucidate the genes associated with host adaptation in planthoppers. Comparative analysis of planthoppers’ responses to different diets would improve our knowledge of host adaptation regarding herbivores insects.Results: In the present study, we analyzed the gene expression change of SBPH that transferred from rice plants to wheat plants in a short term (rSBPH vs tSBPH) and colonized on wheat plants for a long term (rSBPH vs wSBPH). The results showed that the majority of differentially expressed genes in SBPH showed similar expression change between short-term transfer and long-term colonization. Based on the comparative analysis of BPH and SBPH after transferring, genes associated with sugar transporters and heat shock proteins varied similarly. However, most of genes were differentially regulated between two species. The detoxification-related genes were upregulated in SBPH after transferring while downregulated in BPH under the same condition. Ribosomal-related genes were downregulated in SBPH after transferring while upregulated in BPH under the same condition.Conclusion: The results of this study provided evidence that host plants played dominant roles in shaping the gene expression, and unfitness of BPH on wheat plants might be determined within 24 hours after transferring. This study deepens our understanding of different host ranges regarding two planthopper species, which may provide a potential strategy for pest management.

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Comparative analysis of different host adaptation in two rice planthopper species

10.21203/rs.2.14809/v2 ◽

2020 ◽

Author(s):

Hai-Jian Huang ◽

Jia-Rong Cui ◽

Xiao-Yue Hong

Keyword(s):

Gene Expression ◽

Comparative Analysis ◽

Insect Pests ◽

Brown Planthopper ◽

Underlying Mechanism ◽

Host Adaptation ◽

Short Term ◽

Expression Change ◽

Rice Plants

Abstract Background: Host adaptation is the major determinant of insect diversification. However, knowledge of different host ranges in very close-related species is scarce. The brown planthopper (Nilaparvata lugens, BPH) and small brown planthopper (Laodelphax striatellus, SBPH) are the most destructive insect pests belonging to Delphacidae. These two species differ in the host range, but the underlying mechanism remains unknown. Comparative analysis of planthoppers’ responses to different diets would improve our knowledge of host adaptation regarding herbivores insects.Results: SBPH could well colonize on rice and wheat plants, while BPH survived on rice plants only. We analyzed the gene expression change of SBPH that transferred from rice plants to wheat plants in a short term (rSBPH vs tSBPH) and colonized on wheat plants for a long term (rSBPH vs wSBPH). We found that the majority of differentially expressed genes in SBPH showed similar expression change between short-term transfer and long-term colonization. Based on the comparative analysis of BPH and SBPH after transferring, genes associated with sugar transporters and heat shock proteins varied similarly. However, most of genes were reciprocally regulated between two species. The detoxification-related genes were upregulated in SBPH after transferring, but downregulated in BPH under the same condition; ribosomal-related genes were down regulated in SBPH after transferring, but upregulated in BPH under the same condition.Conclusion: The results of this study provided evidence that host plants played dominant roles in shaping the gene expression, with alike responding of most genes between short-term transfer and long-term colonization in SBPH. In contrast, the majority of genes in BPH were reciprocally regulated in comparison to those of SBPH, and unfitness of BPH on wheat plants might be determined within 24 hours after transferring. This study deepens our understanding of different host ranges regarding two planthopper species, and may provide a potential strategy for pest management.

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Loci that determine long-term resistance to blast of Russian rice varieties

BIO Web of Conferences ◽

10.1051/bioconf/20202100014 ◽

2020 ◽

Vol 21 ◽

pp. 00014

Author(s):

Julia Goncharova ◽

Olesya Bragin ◽

Sergey Goncharov ◽

Evgeny Kharitonov

Keyword(s):

Pyricularia Oryzae ◽

Main Role ◽

Rice Varieties ◽

Polymorphic Loci ◽

Rice Plants ◽

Primary Screening ◽

Resistant Varieties ◽

Modern Breeding ◽

Resistance Of Varieties

In all rice-growing countries, including Russia, among the economically important, dangerous and harmful diseases of rice, the main role is played blast (pathogen Pyricularia oryzae Cavara). The problem of resistance of rice plants to disease is one of the main problems in modern breeding in most countries. The most effective way to protect rice from blast is using resistant varieties created with marker assistant selection (MAS). The article presents data on chromosomal regions that provide long-term resistance of varieties to pathogen in Russia. The groups of stable and unstable samples significantly differed in the presence of polymorphic loci on the fifth, sixth, eighth, ninth and second chromosomes, which reduces the complexity of evaluating selection material due to the primary screening of gene plasms by variability of resistance loci in the identified chromosomal regions.

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Comparative analysis of diet-associated responses in two rice planthopper species

10.21203/rs.2.14809/v4 ◽

2020 ◽

Author(s):

Hai-Jian Huang ◽

Jia-Rong Cui ◽

Xiao-Yue Hong

Keyword(s):

Gene Expression ◽

Comparative Analysis ◽

High Throughput Sequencing ◽

Insect Pests ◽

Dominant Role ◽

Brown Planthopper ◽

Host Adaptation ◽

Short Term ◽

Rice Plants

Abstract Background: Host adaptation is the primary determinant of insect diversification. However, knowledge of different host ranges in closely related species remains scarce. The brown planthopper (Nilaparvata lugens, BPH) and the small brown planthopper (Laodelphax striatellus, SBPH) are the most destructive insect pests within the family Delphacidae. These two species differ in their host range (SBPH can well colonize rice and wheat plants, whereas BPH survives on only rice plants), but the underlying mechanism of this difference remains unknown. High-throughput sequencing provides a powerful approach for analyzing the association between changes in gene expression and the physiological responses of insects. Therefore, gut transcriptomes were performed to elucidate the genes associated with host adaptation in planthoppers. The comparative analysis of planthopper responses to different diets will improve our knowledge of host adaptation regarding herbivorous insects.Results: In the present study, we analyzed the change in gene expression of SBPHs that were transferred from rice plants to wheat plants over the short term (rSBPH vs tSBPH) or were colonized on wheat plants over the long term (rSBPH vs wSBPH). The results showed that the majority of differentially expressed genes in SBPH showed similar changes in expression for short-term transfer and long-term colonization. Based on a comparative analysis of BPH and SBPH after transfer, the genes associated with sugar transporters and heat-shock proteins showed similar variation. However, most of the genes were differentially regulated between the two species. The detoxification-related genes were upregulated in SBPH after transfer from the rice plants to the wheat plants, but these genes were downregulated in BPH under the same conditions. In contrast, ribosomal-related genes were downregulated in SBPH after transfer, but these genes were upregulated in BPH under the same conditions.Conclusions: The results of this study provide evidence that host plants played a dominant role in shaping gene expression and that the low fitness of BPH on wheat plants might be determined within 24 h after transfer. This study deepens our understanding of different host ranges for the two planthopper species, which may provide a potential strategy for pest management.

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Conceptual short-term memory (CSTM) supports core claims of Christiansen and Chater

Behavioral and Brain Sciences ◽

10.1017/s0140525x15000928 ◽

2016 ◽

Vol 39 ◽

Author(s):

Mary C. Potter

Keyword(s):

Working Memory ◽

Rapid Serial Visual Presentation ◽

Language Comprehension ◽

Short Term Memory ◽

Visual Presentation ◽

Long Term Memory ◽

Short Term ◽

Term Memory ◽

Use Of Knowledge

AbstractRapid serial visual presentation (RSVP) of words or pictured scenes provides evidence for a large-capacity conceptual short-term memory (CSTM) that momentarily provides rich associated material from long-term memory, permitting rapid chunking (Potter 1993; 2009; 2012). In perception of scenes as well as language comprehension, we make use of knowledge that briefly exceeds the supposed limits of working memory.

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Ultrastructural investigations of MDL 19,660-induced effects on the canine platelet and megakaryocyte

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100159412 ◽

1990 ◽

Vol 48 (3) ◽

pp. 374-375

Author(s):

D.E. Loudy ◽

J. Sprinkle-Cavallo ◽

J.T. Yarrington ◽

F.Y. Thompson ◽

J.P. Gibson

Keyword(s):

Antidepressant Drug ◽

Beagle Dogs ◽

Long Term Effects ◽

Short Term ◽

Platelet Counts ◽

Toxicological Studies ◽

Induced Aggregation ◽

Internal Architecture

Previous short term toxicological studies of one to two weeks duration have demonstrated that MDL 19,660 (5-(4-chlorophenyl)-2,4-dihydro-2,4-dimethyl-3Hl, 2,4-triazole-3-thione), an antidepressant drug, causes a dose-related thrombocytopenia in dogs. Platelet counts started to decline after two days of dosing with 30 mg/kg/day and continued to decrease to their lowest levels by 5-7 days. The loss in platelets was primarily of the small discoid subpopulation. In vitro studies have also indicated that MDL 19,660: does not spontaneously aggregate canine platelets and has moderate antiaggregating properties by inhibiting ADP-induced aggregation. The objectives of the present investigation of MDL 19,660 were to evaluate ultrastructurally long term effects on platelet internal architecture and changes in subpopulations of platelets and megakaryocytes.Nine male and nine female beagle dogs were divided equally into three groups and were administered orally 0, 15, or 30 mg/kg/day of MDL 19,660 for three months. Compared to a control platelet range of 353,000- 452,000/μl, a doserelated thrombocytopenia reached a maximum severity of an average of 135,000/μl for the 15 mg/kg/day dogs after two weeks and 81,000/μl for the 30 mg/kg/day dogs after one week.

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Comparison of Auditory, Language, Memory, and Attention Abilities in Children With and Without Listening Difficulties

American Journal of Audiology ◽

10.1044/2020_aja-20-00018 ◽

2020 ◽

Vol 29 (4) ◽

pp. 710-727

Author(s):

Beula M. Magimairaj ◽

Naveen K. Nagaraj ◽

Alexander V. Sergeev ◽

Natalie J. Benafield

Keyword(s):

Auditory Processing ◽

Short Term Memory ◽

Group Differences ◽

Long Term Memory ◽

Short Term ◽

Significant Group ◽

Term Memory ◽

Memory And Attention ◽

Speed And Accuracy

Objectives School-age children with and without parent-reported listening difficulties (LiD) were compared on auditory processing, language, memory, and attention abilities. The objective was to extend what is known so far in the literature about children with LiD by using multiple measures and selective novel measures across the above areas. Design Twenty-six children who were reported by their parents as having LiD and 26 age-matched typically developing children completed clinical tests of auditory processing and multiple measures of language, attention, and memory. All children had normal-range pure-tone hearing thresholds bilaterally. Group differences were examined. Results In addition to significantly poorer speech-perception-in-noise scores, children with LiD had reduced speed and accuracy of word retrieval from long-term memory, poorer short-term memory, sentence recall, and inferencing ability. Statistically significant group differences were of moderate effect size; however, standard test scores of children with LiD were not clinically poor. No statistically significant group differences were observed in attention, working memory capacity, vocabulary, and nonverbal IQ. Conclusions Mild signal-to-noise ratio loss, as reflected by the group mean of children with LiD, supported the children's functional listening problems. In addition, children's relative weakness in select areas of language performance, short-term memory, and long-term memory lexical retrieval speed and accuracy added to previous research on evidence-based areas that need to be evaluated in children with LiD who almost always have heterogenous profiles. Importantly, the functional difficulties faced by children with LiD in relation to their test results indicated, to some extent, that commonly used assessments may not be adequately capturing the children's listening challenges. Supplemental Material https://doi.org/10.23641/asha.12808607

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