Immunization against Plasmodium falciparum with recombinant polypeptides produced in Escherichia coli

1988 ◽  
Vol 10 (6) ◽  
pp. 607-617 ◽  
Author(s):  
ANTHONY A. HOLDER ◽  
ROBERT R. FREEMAN ◽  
STEPHEN C. NICHOLLS
Keyword(s):  
Escherichia Coli ◽  
Plasmodium Falciparum ◽  
Recombinant Polypeptides

scholarly journals The Functional Quality of Soluble Recombinant Polypeptides Produced in Escherichia coli Is Defined by a Wide Conformational Spectrum

2008 ◽  
Vol 74 (23) ◽  
pp. 7431-7433 ◽  
Author(s):  
Mónica Martínez-Alonso ◽  
Nuria González-Montalbán ◽  
Elena García-Fruitós ◽  
Antonio Villaverde
Keyword(s):  
Escherichia Coli ◽  
Green Fluorescent Protein ◽  
Recombinant Proteins ◽  
Fluorescent Protein ◽  
Native Structure ◽  
Functional Quality ◽  
Soluble Aggregates ◽  
Recombinant Polypeptides ◽  
Green Fluorescent

ABSTRACT We have observed that a soluble recombinant green fluorescent protein produced in Escherichia coli occurs in a wide conformational spectrum. This results in differently fluorescent protein fractions in which morphologically diverse soluble aggregates abound. Therefore, the functional quality of soluble versions of aggregation-prone recombinant proteins is defined statistically rather than by the prevalence of a canonical native structure.

scholarly journals Production of recombinant proteins from Plasmodium falciparum in Escherichia coli

Biomédica ◽  
2016 ◽  
Vol 36 ◽  
Author(s):  
Ángela Patricia Guerra ◽  
Eliana Patricia Calvo ◽  
Moisés Wasserman ◽  
Jacqueline Chaparro-Olaya
Keyword(s):  
Escherichia Coli ◽  
Plasmodium Falciparum ◽  
Recombinant Proteins

<p><strong>Introducción.</strong> La producción de proteínas recombinantes es fundamental para el estudio funcional de proteínas de <em>Plasmodium</em> <em>falciparum</em>. Sin embargo, las proteínas recombinantes de <em>P</em>. <em>falciparum</em> están entre las más difíciles de expresar y cuando lo hacen usualmente se agregan dentro de cuerpos de inclusión insolubles.</p><p><strong>Objetivo.</strong> Evaluar la producción de cuatro proteínas de <em>P. falciparum</em>, usando como sistema de expresión dos cepas de <em>Escherichia coli </em>genéticamente modificadas para favorecer la producción de proteínas heterólogas y establecer una reserva de proteínas recombinantes puras y solubles y producir anticuerpos policlonales a partir de ellas.<strong></strong></p><p><strong>Materiales y métodos.</strong> Las proteínas recombinantes, las cuales correspondían a secuencias parciales de PfMyoA (Miosina-A) y PfGAP50 (proteína-asociada a glideosoma-50 kDa) y a las secuencias completas de PfMTIP (proteína de interacción con Miosina-A) y PfGAP45 (proteína asociada a glideosoma-45 kDa), fueron expresadas como proteínas de fusión con GST y luego purificadas y usadas para producir anticuerpos policlonales en ratón.</p><p><strong>Resultados.</strong> La expresión de las proteínas recombinantes fue mucho más eficiente en la cepa BL21-CodonPlus (la cual expresa tRNAs escasos en las bacterias silvestres), que en la cepa BL21-pG-KJE8. En contraste, aunque la cepa BL21-pG-KJE sobreexpresa chaperonas, no redujo la formación de cuerpos de inclusión. <strong>Conclusión.</strong> El uso de cepas de <em>E</em>. <em>coli</em> genéticamente modificadas fue fundamental para alcanzar altos niveles de expresión de las cuatro proteínas recombinantes evaluadas y permitió obtener dos de ellas en forma soluble. La estrategia utilizada permitió expresar cuatro proteínas recombinantes de <em>P</em>. <em>falciparum</em> en cantidad suficiente para inmunizar ratones y producir anticuerpos policlonales, y además conservar proteína pura y soluble de dos de ellas, para ensayos futuros.</p>

scholarly journals Plasmodium falciparum CTP:phosphocholine cytidylyltransferase expressed in Escherichia coli: purification, characterization and lipid regulation

1997 ◽  
Vol 324 (3) ◽  
pp. 903-910 ◽  
Author(s):  
Hye-Jeong YEO ◽  
Marie-Pierre LARVOR ◽  
Marie-Laure ANCELIN ◽  
Henri J. VIAL
Keyword(s):  
Escherichia Coli ◽  
Plasmodium Falciparum ◽  
Oleic Acid ◽  
Recombinant Protein ◽  
Neutral Lipids ◽  
Insoluble Fraction ◽  
Biochemical Properties ◽  
Ctp:Phosphocholine Cytidylyltransferase ◽  
Lipid Regulation

The Plasmodium falciparum CTP:phosphocholine cytidylyltransferase (PfCCT) has been isolated from an overexpressing strain of Escherichia coli. The plasmid pETPfCCT mediated the overexpression of the full-length polypeptide directly. The recombinant protein corresponded to 6–9% of the total cellular proteins and was found essentially in the insoluble membrane fraction. Urea at 6 M was used to solubilize the recombinant protein from the insoluble fraction. The CCT activity was restored upon the removal of urea, and the protein was subsequently purified to homogeneity on a Q-Sepharose column. Approx. 1.4 mg of pure enzyme was obtained from a 250 ml culture of E. coli. Biochemical properties, including in vitro substrate specificity and enzymic characterization, were assessed. The lipid regulation of the recombinant plasmodial CCT activity was characterized for the first time. The Km values were 0.49±0.03 mM (mean±S.E.M.) for phosphocholine and 10.9±0.5 mM for CTP in the presence of lipid activators (oleic acid/egg phosphatidylcholine vesicles), whereas the Km values were 0.66±0.07 mM for phosphocholine and 28.9±0.8 mM for CTP in the absence of lipid activators. The PfCCT activity was stimulated to the same extent in response to egg phosphatidylcholine vesicles containing anionic lipids, such as oleic acid, cardiolipin and phosphatidylglycerol, and was insensitive or slightly sensitive to PC vesicles containing neutral lipids, such as diacylglycerol and monoacylglycerol. Furthermore, the stimulated enzyme activity by oleic acid was antagonized by the cationic aminolipid sphingosine. These lipid-dependence properties place the parasite enzyme intermediately between the mammalian enzymes and the yeast enzyme.

scholarly journals Expression of the Plasmodium falciparum Immunodominant Epitope (NANP)4 on the Surface of Salmonella enterica Using the Autotransporter MisL

2002 ◽  
Vol 70 (7) ◽  
pp. 3611-3620 ◽  
Author(s):  
Fernando Ruiz-Pérez ◽  
Rocío León-Kempis ◽  
Araceli Santiago-Machuca ◽  
Guadalupe Ortega-Pierres ◽  
Eileen Barry ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Plasmodium Falciparum ◽  
Outer Membrane ◽  
Salmonella Enterica ◽  
Secretory Pathway ◽  
Cell Epitope ◽  
Bacterial Strains ◽  
Gram Negative ◽  
Terminal Domain ◽  
Serovar Typhimurium

ABSTRACT Gram-negative bacterial proteins which are exported from the cytosol to the external environment by the type V secretion system are also known as autotransporters. Once translocated to the periplasmic compartment by the sec-dependent general secretory pathway, their C-terminal domain forms a pore through which the N-terminal domain travels to the outer membrane without the need of other accessory proteins. MisL (protein of membrane insertion and secretion) is a protein of unknown function located in the pathogenicity island SPI-3 of Salmonella enterica and classified as an autotransporter due to its high homology to Escherichia coli AIDA-I. In the present work, the MisL C-terminal translocator domain was used to display the immunodominant B-cell epitope of the circumsporozoite protein (CSP) from Plasmodium falciparum on the surface of Salmonella enterica serovar Typhimurium (serovar Typhimurium SL3261) and serovar Typhi (serovar Typhi CVD 908). The MisL β domain was predicted by alignment with AIDA-I, amplified from serovar Typhimurium SL3261, cloned in a plasmid fused to four repeats of the tetrapeptide NANP behind the Escherichia coli heat-labile enterotoxin B subunit signal peptide to ensure periplasmic traffic, and expressed under the control of the anaerobically inducible nirB promoter. The fusion protein was translocated to the outer membrane of both bacterial strains, although the foreign epitope was displayed more efficiently in serovar Typhimurium SL3261, which elicited a better specific antibody response in BALB/c mice. More importantly, antibodies were able to recognize the native CSP in P. falciparum sporozoites. These results confirm that MisL is indeed an autotransporter and that it can be used to express foreign immunogenic epitopes on the surface of gram-negative bacteria.

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