Polarization of PIN3-dependent auxin transport for hypocotyl gravitropic response in Arabidopsis thaliana

The plant hormone auxin controls many aspects of development and acts in part by inducing expression of various genes. Arabidopsis thaliana semidominant shy2 (short hypocotyl) mutations cause leaf formation in dark-grown plants, suggesting that SHY2 has an important role in regulating development. Here we show that the SHY2 gene encodes IAA3, a previously known member of the Aux/IAA family of auxin-induced genes. Dominant shy2 mutations cause amino acid changes in domain II, conserved among all members of this family. We isolated loss-of-function shy2 alleles including a putative null mutation. Gain-of-function and loss-of-function shy2 mutations affect auxin-dependent root growth, lateral root formation, and timing of gravitropism, indicating that SHY2/IAA3 regulates multiple auxin responses in roots. The phenotypes suggest that SHY2/IAA3 may activate some auxin responses and repress others. Models invoking tissue-specificity, feedback regulation, or control of auxin transport may explain these results.

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EIR1, a root-specific protein involved in auxin transport, is required for gravitropism in Arabidopsis thaliana

Genes & Development ◽

10.1101/gad.12.14.2175 ◽

1998 ◽

Vol 12 (14) ◽

pp. 2175-2187 ◽

Cited By ~ 469

Author(s):

C. Luschnig ◽

R. A. Gaxiola ◽

P. Grisafi ◽

G. R. Fink

Keyword(s):

Arabidopsis Thaliana ◽

Auxin Transport ◽

Specific Protein

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Melatonin acts synergistically with auxin to promote lateral root development through fine tuning auxin transport in Arabidopsis thaliana

PLoS ONE ◽

10.1371/journal.pone.0221687 ◽

2019 ◽

Vol 14 (8) ◽

pp. e0221687 ◽

Cited By ~ 4

Author(s):

Shuxin Ren ◽

Laban Rutto ◽

Dennis Katuuramu

Keyword(s):

Arabidopsis Thaliana ◽

Root Development ◽

Lateral Root ◽

Auxin Transport ◽

Fine Tuning ◽

Lateral Root Development

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Influence of Sublethal Concentrations of Herbicides and Growth Regulators on Mouseearcress (Arabidopsis thaliana) Progeny

Weed Science ◽

10.1017/s004317450008259x ◽

1985 ◽

Vol 33 (4) ◽

pp. 430-434 ◽

Cited By ~ 6

Author(s):

Ron Henzell ◽

John Phillips ◽

Peter Diggle

Keyword(s):

Arabidopsis Thaliana ◽

Electron Transport ◽

Plant Growth ◽

Growth Regulators ◽

Growth And Development ◽

Auxin Transport ◽

Photosynthetic Electron Transport ◽

Persistent Effect ◽

Transport Inhibitors ◽

Sublethal Concentrations

The influence of sublethal levels of a number of herbicides and plant growth regulators on the germinability of the seeds and the growth and development of seedlings of mouseearcress [Arabidopsis thaliana(L.) Heynh. ♯ ARBTH] was determined. Only 7 of the 22 chemicals tested had a persistent effect on progeny. Amitrole (3-amino-s-triazole) was one of the most effective compounds. It caused a characteristic bleaching only in shoot tips and pods in parent plants and appeared to act directly on the progeny by accumulation in the seed. Two auxin transport inhibitors, TIBA (2,3,5-triiodobenzoic acid) and CPII (5-O-carboxyphenyl-3-phenylisoxazole), and four of the six photosynthetic electron transport inhibitors included in the study also affected progeny. They appeared to act indirectly by interfering with seed development.

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N-glycan containing a core α1,3-fucose residue is required for basipetal auxin transport and gravitropic response in rice (Oryza sativa )

New Phytologist ◽

10.1111/nph.14031 ◽

2016 ◽

Vol 212 (1) ◽

pp. 108-122 ◽

Cited By ~ 17

Author(s):

Rikno Harmoko ◽

Jae Yong Yoo ◽

Ki Seong Ko ◽

Nirmal Kumar Ramasamy ◽

Bo Young Hwang ◽

...

Keyword(s):

Oryza Sativa ◽

Auxin Transport ◽

Gravitropic Response ◽

Fucose Residue ◽

Basipetal Auxin Transport

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Gravity perception and gravitropic response of inflorescence stems in Arabidopsis thaliana

Advances in Space Research ◽

10.1016/s0273-1177(99)00410-x ◽

1999 ◽

Vol 24 (6) ◽

pp. 763-770 ◽

Cited By ~ 17

Author(s):

H. Fukaki ◽

M. Tasaka

Keyword(s):

Arabidopsis Thaliana ◽

Gravity Perception ◽

Gravitropic Response ◽

Inflorescence Stems

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Antagonistic and auxin-dependent phosphoregulation of columella PIN proteins controls lateral root gravitropic setpoint angle in Arabidopsis

10.1101/594838 ◽

2019 ◽

Author(s):

Suruchi Roychoudhry ◽

Katelyn Sageman-Furnas ◽

Chris Wolverton ◽

Heather L. Goodman ◽

Peter Grones ◽

...

Keyword(s):

Molecular Basis ◽

Lateral Root ◽

Auxin Transport ◽

Lateral Roots ◽

Gravity Vector ◽

Independent Manner ◽

Gravitropic Response ◽

Auxin Distribution ◽

Columella Cells ◽

Molecular Framework

AbstractLateral roots of many species are maintained at non-vertical angles with respect to gravity. These gravitropic setpoint angles (GSAs) are intriguing because their maintenance requires that roots are able to effect gravitropic response both with and against the gravity vector. Here we have used the Arabidopsis lateral root in order to investigate the molecular basis of the maintenance of non-vertical GSAs. We show that gravitropism in the lateral root is angle-dependent and that both upward and downward graviresponse requires auxin transport and the generation of auxin asymmetries consistent with the Cholodny-Went model. We show that the symmetry in auxin distribution in lateral roots growing at GSA can be traced back to a net, balanced polarization of PIN3 and PIN7 auxin transporters in the columella cells. Further, upward and downward graviresponse in lateral roots correlates with corresponding changes in PIN3 and PIN7 polar localisation. Finally, we show that auxin, in addition to driving tropic growth in the lateral root, acts within the columella to regulate GSA via the PIN phosphatase subunit RCN1 in a PIN3-dependent and PIN7-independent manner. Together, these findings provide a molecular framework for understanding gravity-dependent nonvertical growth in Arabidopsis lateral roots.

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MATHEMATICAL MODELING OF AUXIN TRANSPORT IN PROTOXYLEM AND PROTOPHLOEM OF ARABIDOPSIS THALIANA ROOT TIPS

Journal of Bioinformatics and Computational Biology ◽

10.1142/s0219720013400106 ◽

2013 ◽

Vol 11 (01) ◽

pp. 1340010 ◽

Cited By ~ 8

Author(s):

EKATERINA S. NOVOSELOVA ◽

VICTORIA V. MIRONOVA ◽

NADYA A. OMELYANCHUK ◽

FEDOR V. KAZANTSEV ◽

VITALY A. LIKHOSHVAI

Keyword(s):

Arabidopsis Thaliana ◽

Active Transport ◽

Auxin Transport ◽

Positional Information ◽

Root Tip ◽

Root Tips ◽

Auxin Distribution ◽

A Cell ◽

Optimal Values ◽

Main Regulator

Phytohormone auxin is the main regulator of plant growth and development. Nonuniform auxin distribution in plant tissue sets positional information, which determines morphogenesis. Auxin is transported in tissue by means of diffusion and active transport through the cell membrane. There is a number of auxin carriers performing its influx into a cell (AUX\LAX family) or efflux from a cell (PIN, PGP families). The paper presents mathematical models for auxin transport in vascular tissues of Arabidopsis thaliana L.root tip, namely protophloem and protoxylem. Tissue specificity of auxin active transport was considered in these models. There is PIN-mediated auxin efflux in both protoxylem and protophloem, but AUX1-mediated influx exists only in protophloem. Optimal values of parameters were adjusted for model solutions to fit the experimentally observed auxin distributions in the root tip. Based on simulation results we predicted that shoot-derived auxin flow to protophloem is lower than one to protoxylem, and the efficiency of PIN-mediated auxin transport in protophloem is higher than in protoxylem. In summary, our simulation showed that despite the same auxin distribution pattern, provascular tissues in the root tip differ in dynamics of auxin transport.

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