Evidence for serum immunoglobulin G (IgG) antibody responses in Macaca fascicularis identified by monoclonal antibodies to human IgG subclasses

1997 ◽  
Vol 12 (4) ◽  
pp. 193-203 ◽  
Author(s):  
J. L. Ebersole ◽  
G. R. Bauman ◽  
S. E. Cox O'Dell ◽  
A. Giardino
Keyword(s):  
Monoclonal Antibodies ◽  
Immunoglobulin G ◽  
Macaca Fascicularis ◽  
Igg Subclasses ◽  
Antibody Responses ◽  
Serum Immunoglobulin ◽  
Igg Antibody ◽  
Human Igg

scholarly journals Longitudinal Analysis of Cryptosporidium Species-Specific Immunoglobulin G Antibody Responses in Peruvian Children

2006 ◽  
Vol 13 (1) ◽  
pp. 123-131 ◽  
Author(s):  
Jeffrey W. Priest ◽  
Caryn Bern ◽  
Lihua Xiao ◽  
Jacquelin M. Roberts ◽  
James P. Kwon ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Antibody Responses ◽  
Health Interventions ◽  
Older Children ◽  
Serum Samples ◽  
Igg Antibody ◽  
Specific Immunoglobulin ◽  
Antibody Assays ◽  
Species Specific ◽  
Antibody Levels

ABSTRACT Cryptosporidium species are ubiquitous in the environment and are frequently detected in the stools of children who live where sanitation conditions are poor. To better characterize the immune response to these parasites, we monitored immunoglobulin G (IgG) antibody levels in a cohort of children from Lima, Peru. Two new enzyme-linked immunosorbent assays based on the C. parvum (bovine, subtype IIa) Iowa strain 17-kDa and 27-kDa antigens were used to measure IgG antibody levels in longitudinal serum samples. Antibody responses were detected during infections with C. parvum, C. felis, and C. meleagridis and with four different subtypes of C. hominis. We also noted that the magnitude of the antibody response was related to the number of previous infections and that older children generally had higher levels of antibodies to the two C. parvum antigens. Antibody responses were not associated with infections with either Cyclospora sp. or Giardia sp. We believe the antibody assays will be important tools for monitoring the success of future public health interventions.

scholarly journals FVIII inhibitor IgG subclass and FVIII polypeptide specificity determined by immunoblotting

Blood ◽  
1987 ◽  
Vol 69 (5) ◽  
pp. 1475-1480 ◽  
Author(s):  
CA Fulcher ◽  
S de Graaf Mahoney ◽  
TS Zimmerman
Keyword(s):  
Monoclonal Antibodies ◽  
Factor Viii ◽  
Igg Subclasses ◽  
Radial Immunodiffusion ◽  
Igg Subclass ◽  
Human Igg ◽  
Immunoglobulin Class ◽  
Fviii Inhibitor

Abstract We used immunoblotting of purified factor VIII coagulant protein (FVIII) to localize FVIII inhibitor epitopes in 76 inhibitor plasmas to either the 92-kd FVIII polypeptide (and its 54-kd and/or 44-kd thrombin fragments), the 80-kd polypeptide (and its 72-kd thrombin fragment), or both of these polypeptides. We also used immunoblotting to examine the immunoglobulin class and subclass content of 12 inhibitors with monoclonal antibodies specific for human IgG subclasses and IgM. Seven hemophilic (alloantibody) and five spontaneous (autoantibody) inhibitors contained IgG-1 and IgG-4 antibody; one of the spontaneous inhibitors also contained IgG-3. In one hemophilic inhibitor, the IgG-4 component reacted strongly with the 92-kd and 80-kd polypeptides, whereas the IgG-1 component reacted only minimally with the 92-kd polypeptide although its reactivity with the 80-kd polypeptide was strong. Another hemophilic inhibitor was affinity purified and subjected to quantitative radial immunodiffusion, and the presence of IgG-1 and IgG-4 antibody was confirmed. We conclude that the inhibitors examined are not monoclonal but are probably of restricted polyclonal origin and that different IgG subclasses in an inhibitor plasma can have different degrees of FVIII polypeptide reactivity.

scholarly journals Immunoglobulin G (IgG) Subclass and IgE Responses in Human Paragonimiases Caused by Three Different Species

1998 ◽  
Vol 5 (4) ◽  
pp. 474-478 ◽  
Author(s):  
Yoon Kong ◽  
Akira Ito ◽  
Hyun-Jong Yang ◽  
Young-Bae Chung ◽  
Shiro Kasuya ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Positive Reaction ◽  
Immunoglobulin G ◽  
Igg Subclasses ◽  
Serum Immunoglobulin ◽  
Igg Subclass ◽  
Healthy Controls ◽  
Paragonimus Westermani ◽  
Egg Extracts ◽  
Specific Reactions

ABSTRACT In 40 cases of human paragonimiases caused by Paragonimus westermani (20 cases), P. miyazakii (10 cases), andP. skrjabini (10 cases), responses of serum immunoglobulin G (IgG), IgG subclasses, and IgE were analyzed by immunoblotting with crude antigens prepared from egg, 4-week-old juvenile, and adult forms of P. westermani. The 32- and 35-kDa proteins in the adult extracts showed specific reactions regardless of the causative species (39 of 40 cases; 98%). Sera of patients infected with P. westermani and P. miyazakii reacted strongly with the 28-, 46-, and 94-kDa proteins of egg extracts, while those from patients infected with P. skrjabini reacted faintly. No sera from patients with other trematodiases (0 of 15 cases), cestodiases (0 of 20 cases), or lung cancer (0 of 5 cases) or from healthy controls (0 of 10 individuals) showed positive reactions. Analysis by IgG subclass revealed that IgG4 (33 of 40 cases; 83%) and IgG1 (29 of 40 cases; 73%) antibodies in the patient sera recognized the 32- and 35-kDa proteins predominantly. IgG3 reaction was found in 50% (10 of 20 cases) and 30% (3 of 10 cases) of the sera of patients infected with P. westermani and P. miyazakii, respectively. In an IgE immunoblot, 83% (33 of 40 cases) of the sera from paragonimiasis patients reacted with the 32- and 35-kDa proteins while no sera from patients with heterologous diseases and healthy controls showed a positive reaction. Both 32- and 35-kDa proteins in adult extracts of P. westermani were highly reliable for serodiagnosis of human paragonimiases.

Isoelectric focusing-affinity immunoblot analysis of mouse monoclonal antibodies to the four human IgG subclasses

1987 ◽  
Vol 8 (3) ◽  
pp. 127-134 ◽  
Author(s):  
Robert G. Hamilton ◽  
Marianne Roebber ◽  
Charles B. Reimer ◽  
L. Scott Rodkey
Keyword(s):  
Monoclonal Antibodies ◽  
Isoelectric Focusing ◽  
Immunoblot Analysis ◽  
Igg Subclasses ◽  
Human Igg

scholarly journals Serum Immunoglobulin G Antibody Responses to Bordetella pertussis Lipooligosaccharide and B.parapertussis Lipopolysaccharide in Children with Pertussis and Parapertussis

2001 ◽  
Vol 8 (5) ◽  
pp. 1015-1017 ◽  
Author(s):  
Birger Trollfors ◽  
Teresa Lagergård ◽  
John Taranger ◽  
Elisabet Bergfors ◽  
Rachel Schneerson ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Bordetella Pertussis ◽  
Immunoglobulin G ◽  
Antibody Responses ◽  
Serum Immunoglobulin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Bordetella Parapertussis ◽  
The Individual

ABSTRACT Serum immunoglobulin G (IgG) antibodies against the lipooligosaccharide (LOS) of Bordetella pertussis and the lipopolysaccharide (LPS) of Bordetella parapertussiswere measured by enzyme-linked immunosorbent assay in paired sera from 40 children with pertussis and 14 with parapertussis. Wide differences in the individual responses were noted. Both anti-LOS and -LPS IgG levels increased significantly in the children with pertussis, as did anti-LPS but not anti-LOS in those with parapertussis.

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