Altered gene expression in plants with constitutive expression of a mitochondrial small heat shock protein suggests the involvement of retrograde regulation in the heat stress response

The stress response was studied in suspensions of tubules from immature (IT) and mature (MT) rats after noninjury, heat, oxygen, and anoxia. Under all conditions, IT exhibited more exuberant activation of heat shock transcription factor (HSF) than MT. Characterization of activated HSF in immature cortex revealed HSF1. Also, 2 h after each condition, heat shock protein-72 (HSP-72) mRNA was twofold in IT. As the metabolic response to 45 min of anoxia, 20-min reoxygenation was assessed by measuring O2 consumption (O2C). Basal O2C was manipulated with ouabain, nystatin, and carbonylcyanide p-chloromethyoxyphenylhydrazone (CCCP). Basal O2C in IT were one-half the value of MT. After anoxia, basal O2C was reduced by a greater degree in MT. Ouabain reduced O2C to half the basal value in both noninjured and anoxic groups. Basal O2C was significantly stimulated by nystatin but not to the same level following anoxia in MT and IT. Basal O2C was also stimulated by CCCP, but after anoxia, CCCP O2C was significantly less in MT with no decrease in IT, suggesting mitochondria are better preserved in IT. Also, O2C devoted to nontransport activity was better maintained in IT.

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Diet Restriction Induces Heat Shock Gene Expression While Tempering Heat Stress Response in Drosophila melanogaster

The FASEB Journal ◽

10.1096/fasebj.2018.32.1_supplement.523.5 ◽

2018 ◽

Vol 32 (S1) ◽

Author(s):

Jason W. Tresser ◽

Megan Bliss ◽

Hailey Mayweather ◽

Emerson Rustand ◽

Alex Sears ◽

...

Keyword(s):

Gene Expression ◽

Drosophila Melanogaster ◽

Heat Stress ◽

Stress Response ◽

Heat Shock ◽

Heat Shock Gene ◽

Diet Restriction ◽

Heat Stress Response ◽

Shock Gene ◽

Heat Shock Gene Expression

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Genome-wide identification and analysis of the heat shock transcription factor family in moso bamboo (Phyllostachys edulis)

Scientific Reports ◽

10.1038/s41598-021-95899-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Bin Huang ◽

Zhinuo Huang ◽

Ruifang Ma ◽

Jialu Chen ◽

Zhijun Zhang ◽

...

Keyword(s):

Heat Stress ◽

Stress Response ◽

Heat Shock ◽

Gene Family ◽

Regulatory Elements ◽

Moso Bamboo ◽

Naphthalene Acetic Acid ◽

Plant Responses ◽

Heat Stress Response ◽

Regulatory Pathways

AbstractHeat shock transcription factors (HSFs) are central elements in the regulatory network that controls plant heat stress response. They are involved in multiple transcriptional regulatory pathways and play important roles in heat stress signaling and responses to a variety of other stresses. We identified 41 members of the HSF gene family in moso bamboo, which were distributed non-uniformly across its 19 chromosomes. Phylogenetic analysis showed that the moso bamboo HSF genes could be divided into three major subfamilies; HSFs from the same subfamily shared relatively conserved gene structures and sequences and encoded similar amino acids. All HSF genes contained HSF signature domains. Subcellular localization prediction indicated that about 80% of the HSF proteins were located in the nucleus, consistent with the results of GO enrichment analysis. A large number of stress response–associated cis-regulatory elements were identified in the HSF upstream promoter sequences. Synteny analysis indicated that the HSFs in the moso bamboo genome had greater collinearity with those of rice and maize than with those of Arabidopsis and pepper. Numerous segmental duplicates were found in the moso bamboo HSF gene family. Transcriptome data indicated that the expression of a number of PeHsfs differed in response to exogenous gibberellin (GA) and naphthalene acetic acid (NAA). A number of HSF genes were highly expressed in the panicles and in young shoots, suggesting that they may have functions in reproductive growth and the early development of rapidly-growing shoots. This study provides fundamental information on members of the bamboo HSF gene family and lays a foundation for further study of their biological functions in the regulation of plant responses to adversity.

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Allele-Specific Expression and Evolution of Gene Regulation Underlying Acute Heat Stress Response and Local Adaptation in the Copepod Tigriopus californicus

Journal of Heredity ◽

10.1093/jhered/esaa044 ◽

2020 ◽

Vol 111 (6) ◽

pp. 539-547

Author(s):

Sumaetee Tangwancharoen ◽

Brice X Semmens ◽

Ronald S Burton

Keyword(s):

Gene Expression ◽

Heat Stress ◽

Stress Response ◽

Local Adaptation ◽

F1 Hybrids ◽

Specific Expression ◽

Heat Stress Response ◽

Acute Heat Stress ◽

Number Of Genes ◽

Allele Specific

Abstract Geographic variation in environmental temperature can select for local adaptation among conspecific populations. Divergence in gene expression across the transcriptome is a key mechanism for evolution of local thermal adaptation in many systems, yet the genetic mechanisms underlying this regulatory evolution remain poorly understood. Here we examine gene expression in 2 locally adapted Tigriopus californicus populations (heat tolerant San Diego, SD, and less tolerant Santa Cruz, SC) and their F1 hybrids during acute heat stress response. Allele-specific expression (ASE) in F1 hybrids was used to determine cis-regulatory divergence. We found that the number of genes showing significant allelic imbalance increased under heat stress compared to unstressed controls. This suggests that there is significant population divergence in cis-regulatory elements underlying heat stress response. Specifically, the number of genes showing an excess of transcripts from the more thermal tolerant (SD) population increased with heat stress while that number of genes with an SC excess was similar in both treatments. Inheritance patterns of gene expression also revealed that genes displaying SD-dominant expression phenotypes increase in number in response to heat stress; that is, across loci, gene expression in F1’s following heat stress showed more similarity to SD than SC, a pattern that was absent in the control treatment. The observed patterns of ASE and inheritance of gene expression provide insight into the complex processes underlying local adaptation and thermal stress response.

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