scholarly journals Abundant Messenger RNA Sequences Common to Transplanted Mouse Tumours of Different Tissue Origins

2005 ◽  
Vol 128 (1) ◽  
pp. 193-197
Author(s):  
Gilles MERCIER ◽  
Martine GUILLIER ◽  
Jacques HAREL
Keyword(s):  
Messenger Rna ◽  
Rna Sequences

scholarly journals Expression of rabbit ventricular alpha-myosin heavy chain messenger RNA sequences in atrial muscle.

1984 ◽  
Vol 259 (10) ◽  
pp. 6674-6680
Author(s):  
A M Sinha ◽  
D J Friedman ◽  
J M Nigro ◽  
S Jakovcic ◽  
M Rabinowitz ◽  
...  
Keyword(s):  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Messenger Rna ◽  
Rna Sequences ◽  
Atrial Muscle

scholarly journals Transcriptome-wide analysis of PGC-1α–binding RNAs identifies genes linked to glucagon metabolic action

2020 ◽  
Vol 117 (36) ◽  
pp. 22204-22213 ◽  
Author(s):  
Clint D. J. Tavares ◽  
Stefan Aigner ◽  
Kfir Sharabi ◽  
Shashank Sathe ◽  
Beste Mutlu ◽  
...  
Keyword(s):  
Messenger Rna ◽  
Large Fraction ◽  
Glucose Production ◽  
Peroxisome Proliferator ◽  
Rna Sequences ◽  
Peroxisome Proliferator Activated Receptor ◽  
Rna Recognition Motifs ◽  
Transcript Levels ◽  
Recognition Motifs ◽  
Metabolic Action

The peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) is a transcriptional coactivator that controls expression of metabolic/energetic genes, programming cellular responses to nutrient and environmental adaptations such as fasting, cold, or exercise. Unlike other coactivators, PGC-1α contains protein domains involved in RNA regulation such as serine/arginine (SR) and RNA recognition motifs (RRMs). However, the RNA targets of PGC-1α and how they pertain to metabolism are unknown. To address this, we performed enhanced ultraviolet (UV) cross-linking and immunoprecipitation followed by sequencing (eCLIP-seq) in primary hepatocytes induced with glucagon. A large fraction of RNAs bound to PGC-1α were intronic sequences of genes involved in transcriptional, signaling, or metabolic function linked to glucagon and fasting responses, but were not the canonical direct transcriptional PGC-1α targets such as OXPHOS or gluconeogenic genes. Among the top-scoring RNA sequences bound to PGC-1α wereFoxo1,Camk1δ,Per1,Klf15,Pln4,Cluh,Trpc5,Gfra1, andSlc25a25. PGC-1α depletion decreased a fraction of these glucagon-induced messenger RNA (mRNA) transcript levels. Importantly, knockdown of several of these genes affected glucagon-dependent glucose production, a PGC-1α–regulated metabolic pathway. These studies show that PGC-1α binds to intronic RNA sequences, some of them controlling transcript levels associated with glucagon action.

scholarly journals Unweaving the Meanings of Messenger RNA Sequences

2006 ◽  
Vol 23 (2) ◽  
pp. 150-151 ◽  
Author(s):  
Roderic Guigó ◽  
Juan Valcárcel
Keyword(s):  
Messenger Rna ◽  
Rna Sequences

The messenger RNA sequences in growing and resting mouse fibroblasts

Cell ◽  
1975 ◽  
Vol 6 (2) ◽  
pp. 197-206 ◽  
Author(s):  
Jeffrey G. Williams ◽  
Sheldon Penman
Keyword(s):  
Messenger Rna ◽  
Rna Sequences ◽  
Mouse Fibroblasts

scholarly journals Transcription of bacteriophage T4 deoxyribonucleic acid in vitro

1969 ◽  
Vol 115 (3) ◽  
pp. 353-361 ◽  
Author(s):  
John O. Bishop ◽  
Forbes W. Robertson
Keyword(s):  
Rna Polymerase ◽  
Messenger Rna ◽  
Rna Synthesis ◽  
Deoxyribonucleic Acid ◽  
Bacteriophage T4 ◽  
Rna Sequences ◽  
Experimental Conditions ◽  
E Coli ◽  
New Methods

1. RNA was synthesized in vitro from a template of bacteriophage T4 DNA, in the presence of Mn2+. A comparison was made of the RNA synthesized by purified RNA polymerase from two sources, Micrococcus lysodeikticus and Escherichia coli; these are referred to as Micrococcus cRNA and E. coli cRNA respectively (where cRNA indicates RNA synthesized in vitro by using purified RNA polymerase and a DNA primer). 2. Both types of RNA were self-complementary as judged by resistance to digestion with ribonuclease after self-annealing, Micrococcus cRNA being more self-complementary (40%) than was E. coli cRNA (30%). The cRNA was found to be much less self-complementary if Mg2+ was present during RNA synthesis instead of Mn2+. 3. Micrococcus cRNA hybridized with a larger part of bacteriophage T4 DNA than did E. coli cRNA. The E. coli cRNA competed with only part (70%) of the Micrococcus cRNA in hybridization-competition experiments. It is concluded that more sequences of bacteriophage T4 DNA are transcribed by Micrococcus polymerase than by E. coli polymerase. 4. The RNA sequences synthesized by Micrococcus RNA polymerase but not by E. coli RNA polymerase are shown by hybridization competition to compete with specifically late bacteriophage T4 messenger RNA sequences. The relevance of this finding to the control of transcription is discussed. 5. In an Appendix, new methods are described for the analysis of hybridization-saturation and -competition experiments. Particular attention is paid to the effects produced if different RNA sequences are present at different relative concentrations. 6. By using cRNA isolated from an enzymically synthesized DNA–RNA hybrid, it is estimated that, of the DNA that is complementary to cRNA, only about half can become hybridized with cRNA under the experimental conditions used.

scholarly journals Identification of renin and angiotensinogen messenger RNA sequences in mouse and rat brains.

Hypertension ◽  
1986 ◽  
Vol 8 (6) ◽  
pp. 544-548 ◽  
Author(s):  
V J Dzau ◽  
J Ingelfinger ◽  
R E Pratt ◽  
K E Ellison
Keyword(s):  
Messenger Rna ◽  
Rna Sequences

scholarly journals Sequences responsible for intracellular localization of beta-actin messenger RNA also affect cell phenotype.

1994 ◽  
Vol 127 (2) ◽  
pp. 441-451 ◽  
Author(s):  
E H Kislauskis ◽  
X Zhu ◽  
R H Singer
Keyword(s):  
Messenger Rna ◽  
Intracellular Localization ◽  
Cell Phenotype ◽  
Cell Periphery ◽  
Actin Stress Fiber ◽  
Rna Sequences ◽  
Protein Levels ◽  
Full Activity ◽  
Beta Actin ◽  
Actin Mrna

We have characterized the structure and function of RNA sequences that direct beta-cytoplasmic actin mRNA to the cell periphery were mapped to two segments of 3'-untranslated region by expression of LacZ/beta-actin chimeric mRNAs in chicken embryo fibroblasts (CEFs). A 54-nt segment, the "RNA zipcode," and a homologous but less active 43-nt segment each localized beta-galactosidase activity to the leading lamellae. This zipcode contains the full activity, and mutations or deletions within it reduce, but do not eliminate, its activity, indicating that several motifs contribute to the activity. Two of these motifs, when multimerized, can regenerate almost full activity. These sequences are highly conserved in evolution, since the human beta-actin zipcode, positioned identically in the 3'UTR localizes equally well in chicken cells. Complementary phosphorothioate oligonucleotides against the zipcode delocalized endogenous beta-actin mRNA, whereas those complementary to the region just outside the zipcode, or sense oligonucleotides, did not. Actin mRNA or protein levels were unaffected by the antisense treatments, but a dramatic change in lamellipodia structure, and actin stress fiber organization was observed using the same antizipcode oligonucleotides which delocalized the mRNA. Hence, discrete 3'UTR sequences direct beta-actin isoform synthesis to the leading lamellae and affect cell morphology, presumably through the actin cytoskeleton.

Antisense strategies and therapeutic applications

1996 ◽  
Vol 53 (2) ◽  
pp. 151-160 ◽  
Author(s):  
Putnam David A.
Keyword(s):  
Virus Infection ◽  
Messenger Rna ◽  
Therapeutic Agents ◽  
Water Soluble ◽  
Rna Sequences ◽  
Potential Applications ◽  
Antisense Approach ◽  
Simplex Virus

The concepts underlying the antisense approach to disease therapy are discussed, and potential applications are examined. Antisense therapeutic agents bind to DNA or RNA sequences, biocking the synthesis of cellular proteins with unparalleled specificity. Transcription and translation are the two processes with which the agents interfere. There are three major classes of antisense agents: antisense sequences, commonly called antisense oligonucleotides; antigene sequences; and ribozymes. Antisense sequences are derivatives of nucleic acids that hybridize cytosolic messenger RNA (mRNA) sense strands through hydrogen bonding to complementary nucleic acid bases. Antigene sequences hybridize double-stranded DNA in the nucleus, forming triple helixes. Ribozymes, rather than inhibiting protein synthesis simply by binding to a single targeted mRNA. combine enzymatic processes with the specificity of antisense Iwse pairing, creating a molecule that can incapacitate multiple targeted niRNAs. Anti-sense therapeutic agents are being investigated in vitro and in vivo for use in treating human immunodeficiency virus infection, hepatitis B virus infection, herpes simplex virus infection, papillomavirus infection, cancer, restenosis, rheumatoid arthritis, and allergic disorders. Although many results are preliminary, some are promising and has e led to clinical trials. A major goal in developing methods of delivering antisense agents is to reduce their susceptibility to nucleases while retaining their ability to bind to targeted sites. Modification of the phosphodiester linkages in oligonucleotides can lend the sequences enzymatic stability without affecting their binding capacities. Carrier systems designed to protect the antisense structure and improve passage through the cell membrane include liposomes, water-soluble polyrners, and nanoparticles. The pharmacokinetics of anti-sense agents are under investigation. Antisense therapeutic agents have the potential to become an integral part of medicinal regimens.

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