Assignment of the 600-MHz 1H-NMR spectrum of amicyanin from Thiobacillus versutus by two-dimensional NMR methods provides information on secondary structure

1991 ◽  
Vol 201 (3) ◽  
pp. 695-702 ◽  
Author(s):  
Arjen LOMMEN ◽  
Sybren WIJMENGA ◽  
Cees W. HILBERS ◽  
Gerard W. CANTERS
Keyword(s):  
Secondary Structure ◽  
1H Nmr ◽  
Two Dimensional ◽  
Nmr Spectrum ◽  
Thiobacillus Versutus ◽  
Nmr Methods

Study on the Inclusion Behavior of Cucurbit [n] uril with Phenylalanine

2011 ◽  
Vol 197-198 ◽  
pp. 1153-1156
Author(s):  
Ning Chen ◽  
Ya Bin Li
Keyword(s):  
1H Nmr ◽  
Interaction Mechanism ◽  
Acetate Buffer Solution ◽  
Molar Ratio ◽  
High Concentration ◽  
Buffer Solution ◽  
Visible Absorption ◽  
Nmr Spectrum ◽  
Application Range ◽  
Uv Visible

The characteristics of host-guest complexes between cucurbit[n]uril (CB [n]) and phenylalanine were investigated by UV-visible absorption spectroscopy in acetate buffer solution at room temperature. It was found that the UV-visible absorption increased steadily with constantly dropping the high concentration of cucurbit[6]uril (CB [6]) and cucurbit[8]uril (CB [8]) in the phenylalanine solution which indicates that there are some interaction betweenCB [n] and phenylalanine.Then CB [6] and phenylalanine at molar ratio of 1:1 to weigh while CB [8] and phenylalanine at molar ratio of 1:2, respectively, are both demonstrated by 1H NMR spectra. 1H NMR spectrum of complexes was obtained, indicating an enthalpic driving force for host-guest complexes. The possible interaction mechanism and inclusion mode were also discussed. This work may extend the application range of CB [n] in supramolecular and pharmaceutical analysis.

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