An upstream region of the rat spermatogenesis-specific heat-shock-like Hst70 gene confers testis-specific expression in transgenic mice

1993 ◽  
Vol 212 (1) ◽  
pp. 137-143 ◽  
Author(s):  
Jan WISNIEWSKI ◽  
Marek MALEZEWSKI ◽  
Zdzislaw KRAWCZYK ◽  
Lashitew GEDAMU
Keyword(s):  
Heat Shock ◽  
Transgenic Mice ◽  
Specific Heat ◽  
Upstream Region ◽  
Specific Expression ◽  
Rat Spermatogenesis ◽  
Testis Specific Expression

scholarly journals A 4.2 kb Upstream Region of the Human Corneodesmosin Gene Directs Site-Specific Expression in Hair Follicles and Hyperkeratotic Epidermis of Transgenic Mice

2004 ◽  
Vol 122 (3) ◽  
pp. 730-738 ◽  
Author(s):  
Hélène Gallinaro ◽  
Nathalie Jonca ◽  
Lutz Langbein ◽  
Christian Vincent ◽  
Michel Simon ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Hair Follicles ◽  
Upstream Region ◽  
Specific Expression ◽  
Site Specific

Testis-Specific Expression of the Rat Histone H1t Gene in Transgenic Mice

Biochemistry ◽  
1995 ◽  
Vol 34 (27) ◽  
pp. 8733-8743 ◽  
Author(s):  
Jane M. vanWert ◽  
Steven A. Wolfe ◽  
Sidney R. Grimes
Keyword(s):  
Transgenic Mice ◽  
Specific Expression ◽  
Testis Specific Expression ◽  
Histone H1t

Mouse testis Pdha-2 promoter upstream sequences confer tissue-and temporal-specific activity in transgenic mice

1994 ◽  
Vol 6 (5) ◽  
pp. 599 ◽  
Author(s):  
RC Iannello ◽  
JC Young ◽  
S Sumarsono ◽  
MJ Tymms ◽  
I Kola
Keyword(s):  
Transgenic Mice ◽  
Specific Activity ◽  
Pyruvate Dehydrogenase Complex ◽  
Somatic Tissue ◽  
Specific Expression ◽  
Complex Process ◽  
Specific Manner ◽  
Cat Gene ◽  
Testis Specific Expression

Spermatogenesis is a complex process requiring the coordinate expression of a number of testis-specific genes. One of these, Pdha-2, codes for the murine testis-specific isoform of the E1 alpha subunit of the pyruvate dehydrogenase complex. To elucidate the mechanisms regulating its expression in vivo, we have begun to investigate the Pdha-2 promoter in transgenic mice. In this paper, a construct containing 3.0 kb of promoter and upstream sequences is reported to be sufficient for directing the testis-specific expression of a CAT reporter gene in mice harbouring the transgene. Similarly to the endogenous Pdha-2, the CAT gene is expressed in testis in a stage-specific manner. However, the 3.0-kb Pdha-2 promoter is not active in somatic tissue suggesting that repressor elements may be present within these sequences.

scholarly journals Molecular Dissection of DNA Sequences and Factors Involved in Slow Muscle-Specific Transcription

2001 ◽  
Vol 21 (24) ◽  
pp. 8490-8503 ◽  
Author(s):  
Soledad Calvo ◽  
Detlef Vullhorst ◽  
Pratap Venepally ◽  
Jun Cheng ◽  
Irina Karavanova ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transgenic Mice ◽  
Dna Sequences ◽  
Muscle Development ◽  
Fiber Type ◽  
Regulatory Element ◽  
Slow Muscle ◽  
Upstream Region ◽  
Fiber Types ◽  
Specific Expression

ABSTRACT Transcription is a major regulatory mechanism for the generation of slow- and fast-twitch myofibers. We previously identified an upstream region of the slow TnI gene (slow upstream regulatory element [SURE]) and an intronic region of the fast TnI gene (fast intronic regulatory element [FIRE]) that are sufficient to direct fiber type-specific transcription in transgenic mice. Here we demonstrate that the downstream half of TnI SURE, containing E box, NFAT, MEF-2, and CACC motifs, is sufficient to confer pan-skeletal muscle-specific expression in transgenic mice. However, upstream regions of SURE and FIRE are required for slow and fast fiber type specificity, respectively. By adding back upstream SURE sequences to the pan-muscle-specific enhancer, we delineated a 15-bp region necessary for slow muscle specificity. Using this sequence in a yeast one-hybrid screen, we isolated cDNAs for general transcription factor 3 (GTF3)/muscle TFII-I repeat domain-containing protein 1 (MusTRD1). GTF3 is a multidomain nuclear protein related to initiator element-binding transcription factor TF II-I; the genes for both proteins are deleted in persons with Williams-Beuren syndrome, who often manifest muscle weakness. Gel retardation assays revealed that full-length GTF3, as well as its carboxy-terminal half, specifically bind the bicoid-like motif of SURE (GTTAATCCG). GTF3 expression is neither muscle nor fiber type specific. Its levels are highest during a period of fetal development that coincides with the emergence of specific fiber types and transiently increases in regenerating muscles damaged by bupivacaine. We further show that transcription from TnI SURE is repressed by GTF3 when overexpressed in electroporated adult soleus muscles. These results suggest a role for GTF3 as a regulator of slow TnI expression during early stages of muscle development and suggest how it could contribute to Williams-Beuren syndrome.

Sign in / Sign up

Export Citation Format

Share Document