Progress in xenotransplantation following the introduction of gene-knockout technology

2007 ◽  
Vol 20 (2) ◽  
Author(s):  
Hao-Chih Tai ◽  
Mohamed Ezzelarab ◽  
Hidetaka Hara ◽  
David Ayares ◽  
David K.C. Cooper
Keyword(s):  
Gene Knockout ◽  
Knockout Technology ◽  
Gene Knockout Technology

THE EFFECT OF ABA AND IAA PHYTOHORMONES ON THE REDUCING SUGARS AND ENDOGENOUS PHYTOHORMONES CONTENT OF COTTON PLANT UNDER SALIFICATION

1970 ◽  
pp. 57-60
Author(s):  
K. M. Kuldoshova ◽  
А. А. Akhunov ◽  
N. R. Khashimova ◽  
D. T. Babaeva ◽  
М. I. Nurmatova
Keyword(s):  
Salt Stress ◽  
Environmental Factors ◽  
Reducing Sugars ◽  
Prolonged Exposure ◽  
Gene Knockout ◽  
Cotton Variety ◽  
Exogenous Aba ◽  
Endogenous Phytohormones ◽  
Knockout Technology ◽  
Gene Knockout Technology

This article investigates the effect of exogenous phytohormones ABA and IAA on the content of reducing sugars and endogenous phytohormones in cotton seedlings of varieties Porlok-4, obtained on the basis of gene knockout technology and a marker of associated breeding - Ravnak-1. Exogenous phytohormones (ABA and IAA) can significantly change the level of cotton resistance to unfavorable environmental factors. Under the action of exogenous ABA causes an increase of cotton resistance to salinity. Biotechnological cotton variety Ravnak-1 showed sensitivity to prolonged exposure to salinity in combination with phytohormones, increasing the content of reducing sugars and endogenous phytohormones, depending on the duration of salt stress. In the Porlok-4 variety, under the influence of salinity, the content of reducing sugars and endogenous phytohormones had an insignificant increase, due to the inherent adaptive capacity of the variety. The conducted studies allow us to conclude that phytohormones are actively involved in the formation of cotton resistance to the action of unfavorable environmental factors of an abiotic nature.

Quantitating Aortic Atherosclerosis in Rabbits and Mice

1997 ◽  
Vol 3 (S2) ◽  
pp. 317-318
Author(s):  
David A. Sanan ◽  
Dale L. Newland
Keyword(s):  
Gene Knockout ◽  
Laboratory Mouse ◽  
Lipoprotein Metabolism ◽  
Wild Type Mouse ◽  
Mouse Strains ◽  
Chow Diet ◽  
Homologous Genes ◽  
Normal Chow ◽  
Metabolism Gene ◽  
Knockout Technology

Build-up of visible atherosclerotic plaque in the arteries is readily quantifiable. The mouse and the rabbit provide useful models for understanding the pathogenesis of atherosclerosis by investigating the effects of genetic and dietary perturbations.Although the wild type mouse does not develop atherosclerosis, atherosclerosis susceptibility genes have been identified in some laboratory mouse strains which do. Furthermore, transgenic technology and gene targeting have produced genetically modified mice that express various apolipoproteins, enzymes and cofactors involved in human lipoprotein metabolism. Gene “knockout” technology allows transgene expression without interference from homologous genes. One notable “knockout” mouse, deficient in apolipoprotein E, develops spontaneous atherosclerosis on a normal chow diet. Transgenic modulations of the atherosclerotic responses of these highly susceptible mice are more pronounced and easily measured. Small, cheap and fast breeding, mice are convenient animal models. But to make mice susceptible to atherosclerosis, their genetic background has to be so drastically altered that the resulting lipoprotein metabolism may not model the human metabolism accurately enough.

scholarly journals Zebrafish Ddx19 deficiency causes serious apoptosis and cell proliferation

2019 ◽  
Author(s):  
Can Shi ◽  
Linzhu Bao ◽  
Yao Zu ◽  
Jianfeng Ren ◽  
Weiming Li ◽  
...  
Keyword(s):  
Immune Responses ◽  
Cell Apoptosis ◽  
Gene Knockout ◽  
Innate Immune ◽  
Innate Immune Responses ◽  
Dead Box ◽  
Body Development ◽  
Small Head ◽  
Pericardial Edema ◽  
Knockout Technology

Abstract Background: DDX19 is known as for its role in mRNA transport. It is also involved in translation and innate immune responses. However, the function of Ddx19 during body development rarely reported. We know that ddx19 plays an important role in development of organisms, but we don't understand how it affects the process of cell development. Results: Here, we report ddx19-deleted mutation in zebrafish, obtained two types with base deletion of 46 bp and 7 bp using CRISPR/Cas9 gene knockout technology, show morphologic defects such as small head, small eyes, pericardial edema and trunk curvature in 24 hours post fertilization (hpf). The maximum survival time of ddx19 -/- was less than 5 day post fertilization (dpf). In comparison to the wildtype, the mutant embryos showed widespread up-regulation of cell apoptosis, and significant decrease in the number of cells. Conclusions: These data indicate loss of ddx19 is lethal, and is associated with cell apoptosis and proliferation abnormalities in the organism. Our results reveal that ddx19 is essential for the development of zebrafish embryos, which deepens the understanding of the developmental function of DEAD-box genes family.

Production of prion gene knockout cow to prevent spontaneous bovine spongiform encephalopathy

2014 ◽  
Author(s):  
Noboru Manabe ◽  
Ichiro Onoyama ◽  
Junyou Li ◽  
Yutaka Sendai ◽  
Yoshito Aoyagi
Keyword(s):  
Bovine Spongiform Encephalopathy ◽  
Gene Knockout ◽  
Spongiform Encephalopathy ◽  
Prion Gene
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