Seminal Plasma Biochemistry II Seminal Plasma and Spermatozoal Cytidine Monophosphate-Sialic Acid Synthetase and Sialyltransferase Activities

Andrologia ◽  
2009 ◽  
Vol 13 (3) ◽  
pp. 215-224 ◽  
Author(s):  
B. DAUNTER ◽  
J. NEWLANDS
Keyword(s):  
Sialic Acid ◽  
Seminal Plasma ◽  
Plasma Biochemistry ◽  
Cytidine Monophosphate

Organic Constituents of The Seminal Plasma of Balanus Balanus

1963 ◽  
Vol 40 (4) ◽  
pp. 587-594
Author(s):  
H. BARNES
Keyword(s):  
Sialic Acid ◽  
Glutamic Acid ◽  
Total Nitrogen ◽  
Seminal Plasma ◽  
Inorganic Salts ◽  
Organic Substances ◽  
Organic Constituents ◽  
Simple Character ◽  
Soluble Material

1. The seminal plasma of Balanus balanus has been analysed for a number of organic substances including total nitrogen, protein, urea, protein-bound hexose, hexosamine, fucose, ‘sialic acid’, glycogen, and RNA. The material was separated into soluble and insoluble fractions by precipitation with ethanol. 2. A large part of the ethanol-soluble material is inorganic salts and glycine; it contains a small quantity of protein-like material which on hydrolysis yields largely glycine and glutamic acid. 3. The alcohol-precipitated material contains protein, protein-bound hexose, hexosamine, lipids, and RNA. Glycogen, fucose, and ‘sialic acid’ are absent. 4. It is suggested that the plasma contains glycoproteins of a mucoid nature: the absence of both fucose and ‘sialic acid’ suggests that the mucoid may be of a relatively simple character. 5. Attention is drawn to the similarity of this seminal plasma, which is produced in the absence of accessory male organs, to that of higher animals.

Sialic Acid Content of Human Spermatozoa and Seminal Plasma in Relation to Sperm Counts

1983 ◽  
Vol 10 (1) ◽  
pp. 45-46 ◽  
Author(s):  
H. Levinsky ◽  
R. Singer ◽  
M. Barnet ◽  
M. Sagiv ◽  
D. Allalouf
Keyword(s):  
Sialic Acid ◽  
Seminal Plasma ◽  
Acid Content ◽  
Human Spermatozoa ◽  
Sialic Acid Content ◽  
Sperm Counts

Protective effect of esterified glucomannan on aflatoxin-induced changes in testicular function, sperm quality, and seminal plasma biochemistry in rams

2014 ◽  
Vol 81 (3) ◽  
pp. 373-380 ◽  
Author(s):  
Mehmet Bozkurt Ataman ◽  
Hasan Hüseyin Dönmez ◽  
Nurcan Dönmez ◽  
Emrah Sur ◽  
Mustafa Numan Bucak ◽  
...  
Keyword(s):  
Protective Effect ◽  
Seminal Plasma ◽  
Sperm Quality ◽  
Testicular Function ◽  
Plasma Biochemistry ◽  
Induced Changes

scholarly journals Hydroxylation of N-acetylneuraminic Acid Influences the in vivo Tropism of N-linked Sialic Acid-Binding Adeno-Associated Viruses AAV1, AAV5, and AAV6

2021 ◽  
Vol 8 ◽  
Author(s):  
Estrella Lopez-Gordo ◽  
Alejandro Orlowski ◽  
Arthur Wang ◽  
Alan Weinberg ◽  
Susmita Sahoo ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Sialic Acid ◽  
Animal Models ◽  
Animal Studies ◽  
Large Animal ◽  
Knock Out ◽  
Acetylneuraminic Acid ◽  
Formidable Challenge ◽  
Cytidine Monophosphate

Adeno-associated virus (AAV) vectors are promising candidates for gene therapy. However, a number of recent preclinical large animal studies failed to translate into the clinic. This illustrates the formidable challenge of choosing the animal models that promise the best chance of a successful translation into the clinic. Several of the most common AAV serotypes use sialic acid (SIA) as their primary receptor. However, in contrast to most mammals, humans lack the enzyme CMAH, which hydroxylates cytidine monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac) into cytidine monophosphate-N-glycolylneuraminic acid (CMP-Neu5Gc). As a result, human glycans only contain Neu5Ac and not Neu5Gc. Here, we investigate the tropism of AAV1, 5, 6 and 9 in wild-type C57BL/6J (WT) and CMAH knock-out (CMAH−/−) mice. All N-linked SIA-binding serotypes (AAV1, 5 and 6) showed significantly lower transduction of the heart in CMAH−/− when compared to WT mice (5–5.8-fold) and, strikingly, skeletal muscle transduction by AAV5 was almost 30-fold higher in CMAH−/− compared to WT mice. Importantly, the AAV tropism or distribution of expression among different organs was also affected. For AAV1, AAV5 and AAV6, expression in the heart compared to the liver was 4.6–8-fold higher in WT than in CMAH−/− mice, and for AAV5 the expression in the heart compared to the skeletal muscle was 57.3-fold higher in WT than in CMAH−/− mice. These data thus strongly suggest that the relative abundance of Neu5Ac and Neu5Gc plays a role in AAV tropism, and that results obtained in commonly used animal models might not translate into the clinic.

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