Effect of Water Stress on the Lipid and Fatty Acid Composition of Sweet Pepper (Capsicum annuum L.)

PEROXIDASE ACTIVITY AS AN INDICATOR OF WATER STRESS IN SWEET PEPPER PLANTS Antonio Evaldo Klar1; Sidnei Osmar Jadoski2; Giusepina Pace Pereira Lima31Departamento de Engenharia Rural, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista, Botucatu, SP, [email protected] Estadual do Centro Oeste, Centro de Ciências Agrárias e Ambientais, Guarapuava, Pr3Departamento de Bioquímica, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista, Botucatu, SP 1 ABSTRACT The purpose of the study was to evaluate the physiological and biochemical behavior of sweet pepper (Capsicum annuum L.) plants under different soil water availability conditions and the efficiency of the peroxidase (EC. 1.11.1.7) activity as an indicator of water stress in plants. The experiment was carried out at the Faculdade de Ciências Agronômicas – UNESP, Botucatu, SP. Sweet pepper plants were grown for 230 days after transplanting of seedlings and arranged in a completely randomized experimental design with 4 treatments, two irrigation managements (50 and 1500 kPa) and two soil surface managements (presence or absence of black polyethylene covering), and six replications. Physiological activities, such as stomatal transpiration and resistance to water vapor diffusion, were evaluated as well as biochemical activities, such as peroxidase activity and total soluble protein in foliar tissues. It was observed that soil water availability may lead to physiological and biochemical alterations in plants. Successive water stress cycles may promote the development of characteristics responsible for improving plant tolerance to periods of low water availability. The peroxidase enzyme activity showed to be an efficient indicator of water stress in sweet pepper plants. KEYWORDS: enzymatic activity; soil moisture; vegetable physiology; soluble protein; Capsicum annuum L. KLAR, A. E.; JADOSKI, S. O.; LIMA, G. P. P. A PEROXIDASE COMO INDICADOR DE DÉFICITS HÍDRICOS EM PLANTAS DE PIMENTÃO. 2 RESUMO O objetivo do presente estudo foi avaliar o comportamento fisiológico e bioquímico do pimentão (Capsicum annuum L) sob diferentes condições de disponibilidade de água no solo, além da eficiência da peroxidase (EC 1.11.1.7) como indicador de déficits hídricos nas plantas. O experimento foi desenvolvido na Faculdade de Ciências Agronômicas – UNESP, Botucatu, SP, durante 230 dias após o transplante das plântulas em condições de ambiente protegido com quatro tratamentos: dois níveis de irrigação (-50 e – 1500 kPa como potenciais mínimos de água do solo) e dois manejos de cobertura do solo (com e sem cobertura de lonas de polietileno preto) com seis repetições. As atividades fisiológicas, como a resistência difusiva dos estômatos e a transpiração foram avaliadas, assim como a peroxidase e a proteina solúvel, nos tecidos foliares. Os resultados mostraram que os déficits hídricos alteraram os fatores fisiológicos estudados e promoveram respostas positivas para a tolerância à déficits hídricos. A peroxidase mostrou ser um eficiente indicador de estresse hídrico em plantas de pimentão. UNITERMOS: irrigação, cobertura morta, potencial de água no solo, proteina solúvel.

Download Full-text

Effect of chilling and subsequent storage at 20oC on electrolyte leakage and phospholipid fatty acid composition of tomato pericarp

Physiologia Plantarum ◽

10.1034/j.1399-3054.1993.870412.x ◽

1993 ◽

Vol 87 (4) ◽

pp. 522-527 ◽

Cited By ~ 1

Author(s):

M. Bergevin ◽

G. P. L'Heureux ◽

J. E. Thompson ◽

C. Willemot

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Electrolyte Leakage ◽

Phospholipid Fatty Acid ◽

Phospholipid Fatty Acid Composition ◽

Subsequent Storage

Download Full-text

Platelet Aggregation in Finnish Men and Its Relation to Fatty Acids in Platelets, Plasma and Adipose Tissue

Thrombosis and Haemostasis ◽

10.1055/s-0038-1660071 ◽

1985 ◽

Vol 54 (03) ◽

pp. 563-569 ◽

Cited By ~ 19

Author(s):

M K Salo ◽

E Vartiainen ◽

P Puska ◽

T Nikkari

Keyword(s):

Fatty Acids ◽

Adipose Tissue ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Platelet Aggregation ◽

Acid Composition ◽

Saturated Fatty Acids ◽

Free Living ◽

Ω3 Fatty Acids ◽

Induced Aggregation

SummaryPlatelet aggregation and its relation to fatty acid composition of platelets, plasma and adipose tissue was determined in 196 randomly selected, free-living, 40-49-year-old men in two regions of Finland (east and southwest) with a nearly twofold difference in the IHD rate.There were no significant east-southwest differences in platelet aggregation induced with ADP, thrombin or epinephrine. ADP-induced platelet secondary aggregation showed significant negative associations with all C20-C22 ω3-fatty acids in platelets (r = -0.26 - -0.40) and with the platelet 20: 5ω3/20: 4ω 6 and ω3/ ω6 ratios, but significant positive correlations with the contents of 18:2 in adipose tissue (r = 0.20) and plasma triglycerides (TG) (r = 0.29). Epinephrine-induced aggregation correlated negatively with 20: 5ω 3 in plasma cholesteryl esters (CE) (r = -0.23) and TG (r = -0.29), and positively with the total percentage of saturated fatty acids in platelets (r = 0.33), but had no significant correlations with any of the ω6-fatty acids. Thrombin-induced aggregation correlated negatively with the ω3/6ω ratio in adipose tissue (r = -0.25) and the 20: 3ω6/20: 4ω 6 ratio in plasma CE (r = -0.27) and free fatty acids (FFA) (r = -0.23), and positively with adipose tissue 18:2 (r = 0.23) and 20:4ω6 (r = 0.22) in plasma phospholipids (PL).The percentages of prostanoid precursors in platelet lipids, i. e. 20: 3ω 6, 20: 4ω 6 and 20 :5ω 3, correlated best with the same fatty acids in plasma CE (r = 0.32 - 0.77) and PL (r = 0.28 - 0.74). Platelet 20: 5ω 3 had highly significant negative correlations with the percentage of 18:2 in adipose tissue and all plasma lipid fractions (r = -0.35 - -0.44).These results suggest that, among a free-living population, relatively small changes in the fatty acid composition of plasma and platelets may be reflected in significant differences in platelet aggregation, and that an increase in linoleate-rich vegetable fat in the diet may not affect platelet function favourably unless it is accompanied by an adequate supply of ω3 fatty acids.

Download Full-text

Health Benefits of Pumpkin Seed and Nutrition Profile of 35 Pumpkin Accessions

EDIS ◽

10.32473/edis-hs1312-2017 ◽

2017 ◽

Vol 2017 (6) ◽

Author(s):

Geoffrey M. Meru ◽

Yuqing Fu ◽

Dayana Leyva ◽

Paul Sarnoski ◽

Yavuz Yagiz

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Health Benefits ◽

Pumpkin Seed ◽

Production Practices

This article aims to summise production and nutrition aspects of pumpkin seed. Specifically, it focuses on health benefits of the seeds, production practices and provides data on the oil, protein and fatty acid composition of 35 pumpkin accessions.

Download Full-text