scholarly journals PEROXIDASE ACTIVITY AS AN INDICATOR OF WATER STRESS IN SWEET PEPPER PLANTS

Irriga ◽  
2006 ◽  
Vol 11 (4) ◽  
pp. 441-447 ◽  
Author(s):  
Antonio Evaldo Klar ◽  
Sidnei Osmar Jadoski ◽  
Giusepina Pace Pereira Lima

PEROXIDASE ACTIVITY AS AN INDICATOR OF WATER STRESS IN SWEET PEPPER PLANTS                                             Antonio Evaldo Klar1; Sidnei Osmar Jadoski2; Giusepina Pace Pereira Lima31Departamento de Engenharia Rural, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista, Botucatu, SP, [email protected] Estadual do Centro Oeste,  Centro de Ciências Agrárias e Ambientais, Guarapuava, Pr3Departamento de Bioquímica, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista, Botucatu, SP  1 ABSTRACT             The purpose of the study was to evaluate the physiological and biochemical behavior of sweet pepper (Capsicum annuum L.) plants under different soil water availability conditions and the efficiency of the peroxidase (EC. 1.11.1.7) activity as an indicator of water stress in plants. The experiment was carried out at the Faculdade de Ciências Agronômicas – UNESP, Botucatu, SP. Sweet pepper plants were grown for 230 days after transplanting of seedlings and arranged in a completely randomized experimental design with 4 treatments, two irrigation managements (50 and 1500 kPa) and two soil surface managements (presence or absence of black polyethylene covering), and six replications. Physiological activities, such as stomatal transpiration and resistance to water vapor diffusion, were evaluated as well as biochemical activities, such as peroxidase activity and total soluble protein in foliar tissues. It was observed that soil water availability may lead to physiological and biochemical alterations in plants. Successive water stress cycles may promote the development of characteristics responsible for improving  plant tolerance to periods of low water availability. The peroxidase enzyme activity showed to be an efficient indicator of water stress in sweet pepper plants. KEYWORDS: enzymatic activity; soil moisture; vegetable physiology; soluble protein; Capsicum annuum L.  KLAR, A. E.; JADOSKI, S. O.; LIMA, G. P. P.  A PEROXIDASE COMO INDICADOR DE DÉFICITS HÍDRICOS EM PLANTAS DE PIMENTÃO.  2 RESUMO O objetivo do presente estudo foi avaliar o comportamento fisiológico e bioquímico do pimentão (Capsicum annuum L) sob diferentes condições de disponibilidade de água no solo, além da eficiência da peroxidase (EC 1.11.1.7) como indicador de déficits hídricos nas plantas. O experimento foi desenvolvido na Faculdade de Ciências Agronômicas – UNESP, Botucatu, SP, durante 230 dias após o transplante das plântulas em condições de ambiente protegido com quatro tratamentos: dois níveis de irrigação (-50 e – 1500 kPa como potenciais mínimos de água do solo) e dois manejos de cobertura do solo (com e sem cobertura de lonas de polietileno preto) com seis repetições.  As atividades fisiológicas, como a resistência difusiva dos estômatos e a transpiração foram avaliadas, assim como a peroxidase e a proteina solúvel, nos tecidos foliares. Os resultados mostraram que os déficits hídricos alteraram os fatores fisiológicos estudados e promoveram respostas positivas para a tolerância à déficits hídricos.  A peroxidase mostrou ser um eficiente indicador de estresse hídrico em plantas de pimentão. UNITERMOS: irrigação, cobertura morta, potencial de água no solo, proteina solúvel.

2019 ◽  
Vol 37 (1) ◽  
pp. 82-88
Author(s):  
Alexandre Igor A Pereira ◽  
João de Jesus Guimarães ◽  
João Victor Costa ◽  
Fernando S de Cantuário ◽  
Leandro C Salomão ◽  
...  

ABSTRACT Water stress compromises plant growth. Resistance inducers, such as potassium silicate (K2SiO3), can reduce negative effects of this stress on Solanaceae, Capsicum annuum. Plant height, stem diameter and leaf area may indicate the efficiency of potassium silicate foliarsprayagainst water stress. The aim of this study was to evaluate the growth of sweet pepper plants under water stress and K2SiO3 doses. The experiment was conducted in randomized blocks in a split-plot scheme in space. The treatments consisted of four soil water stresses: 15 kPa (field capacity), 25 (intermediate value), 35 and 45 kPa (water stress) and three doses of potassium silicate (0, 0.4 and 0.8 L 100 L-1 water), acting as resistance inducers to water stress. The resistance inducer maintained greater heights of the sweet pepper plants, under water stress (35 and 45 kPa) at the initial stage [(20 days after transplanting (DAT)]. Smaller plant diameters were observed at 80 and 100 DAT at 35 and 45 kPa. Sprays using K2SiO3 maintained sweet pepper leaf area with higher values, even under stress condition. The soil water tension from 35 kPa limited, in general, the plant growth. Growth responses in Capsicum annuum to K2SiO3, via foliar spraying, varied according to plant age, as well as the growth parameter considered in this experiment.


2000 ◽  
pp. 223-229 ◽  
Author(s):  
S. Delfine ◽  
A. Alvino ◽  
F. Loreto ◽  
M. Centritto ◽  
G. Santarelli

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1790
Author(s):  
Ginés Otálora ◽  
María Piñero ◽  
Jacinta Collado-González ◽  
Josefa López-Marín ◽  
Francisco del Amor

Growers in the cultivated areas where the climate change threatens the agricultural productivity and livelihoods are aware that the current constraints for good quality water are being worsened by heatwaves. We studied the combination of salinity (60 mM NaCl) and heat shock stress (43 °C) in pepper plants (Capsicum annuum L. var. Tamarin) since this can affect physiological and biochemical processes distinctly when compared to separate effects. Moreover, the exogenous application of 0.5 mM salicylic acid (SA) was studied to determine its impacts and the SA-mediated processes that confer tolerance of the combined or stand-alone stresses. Plant growth, leaf Cl− and NO3− concentrations, carbohydrates, and polyamines were analyzed. Our results show that both salinity stress (SS) and heat stress (HS) reduced plant fresh weight, and SA only increased it for HS, with no effect for the combined stress (CS). While SA increased the concentration of Cl− for SS or CS, it had no effect on NO3−. The carbohydrates concentrations were, in general, increased by HS, and were decreased by CS, and for glucose and fructose, by SA. Additionally, when CS was imposed, SA significantly increased the spermine and spermidine concentrations. Thus, SA did not always alleviate the CS and the plant response to CS cannot be directly attributed to the full or partial sum of the individual responses to each stress.


2008 ◽  
Vol 116 (4) ◽  
pp. 343-347 ◽  
Author(s):  
Masahumi Johkan ◽  
Masayuki Oda ◽  
Genjiro Mori

Irriga ◽  
2004 ◽  
Vol 9 (3) ◽  
pp. 217-224 ◽  
Author(s):  
Antonio Evaldo Klar ◽  
Sidnei Osmar Jadoski ◽  
Sidnei Osmar Jadoski

IRRIGATION AND MULCHING MANAGEMENT FOR SWEET PEPPER CROP IN PROTECTED ENVIRONMENT   Antonio Evaldo Klar1; Sidnei Osmar Jadoski21Rural Engineering Department, Agronomic Science College,Paulista State University ,  Botucatu-SP (Brazil),  [email protected] and Veterinarian Sciences Center,  Guarapuava – PR.    1 ABSTRACT  This study was developed over the 1999-2000 agricultural years at the Rural Engineering Department of School of Agronomical Sciences, UNESP, Botucatu, SP, under protected environment. The objective was to evaluate the influence of irrigation and black polyethylene mulch management on the water use and fruit production of sweet pepper crop (Capsicum annuum L., Elisa Hybrid) 230 days after seedling transplant (DAST). The study was divided into two experiments: 1) application of the irrigation at -50 and -1500 kPa minimum soil water potentials with and without mulching on the soil, from 29 to 168 DAST. A randomized experimental design was used with six replications; and 2) severe water deficit in the soil through irrigation suspension and mulching removal (169 to 230 DAST). Drip fertigation was used and soil water was monitored by tensiometers and neutron probe. It was verified that: - the severe drought stress caused leaf senescence and abscission and significantly affected the production and quality of sweet pepper fruits and the Water Use Efficiency (WUE); - the fruit yield and number were higher in the treatments with mulching, therefore polyethylene mulching showed to be an efficient technique to reduce irrigation number and water volume applied. This efficiency was reduced with soil water content decreases based on fruit yield. Pepper plants showed good osmotic adjustment and, consequently, tolerance to water stress.   KEYWORDS: drought stress, polyethylene mulching, soil water potential   KLAR, A. E.; JADOSKI, S. O. IRRIGAÇÃO E COBERTURA MORTA EM PLANTAS DE PIMENTÃO EM AMBIENTE PROTEGIDO   2 RESUMO  O trabalho foi desenvolvido no ano agrícola 1999-2000, em casa de vegetação, no Departamento de Engenharia Rural da Faculdade de Ciências Agronômicas - UNESP, campus de Botucatu, SP. O objetivo foi estudar o efeito de diferentes manejos da água de irrigação e de cobertura de polietileno preto na superfície do solo sobre o consumo de água e produção de frutos da cultura do pimentão (Capsicum annuum L, híbrido Elisa), por um período de 230 dias após o transplante das mudas (DAT). O trabalho foi composto por dois experimentos: I) aplicação dos manejos da  irrigação a 50 kPa e 1500 kPa, com e sem a presença de cobertura de polietileno preto sobre a superfície do solo, para o período de 29 a 168 DAT. O delineamento experimental foi inteiramente casualizado com seis repetições, e II) aplicação de deficiência hídrica severa no solo, através da suspensão das irrigações e retirada da cobertura de polietileno do solo, no período de 169 a 230 DAT. Concluiu-se que a deficiência hídrica afeta a qualidade e a produção dos frutos do pimentão. A utilização de cobertura de polietileno sobre o solo é técnica eficiente para reduzir a necessidade de irrigações e o volume de água a ser aplicado aos cultivos e sua eficiência é reduzida na medida em que diminui o conteúdo de água do solo. Houve significativa influencia no ajuste osmótico pela aplicação dos estresses hídricos nos tratamentos em que o potencial mínimo de água no solo atingiu 1500 kPa.  UNITERMOS: deficiência hídrica, potencial de água do solo, polietileno preto


Author(s):  
F. Casierra-Posada ◽  
◽  
J.A. Camargo-Parra ◽  
M.C. Acosta ◽  
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