Effect of supplementation of multi-microbe probiotic product on growth performance, apparent digestibility, cecal microbiota and small intestinal morphology of broilers

Abstract The objective of this study was to investigate the effects of xylo-oligosaccharides (XOS) supplementation on growth performance, serum parameters, small intestinal morphology, intestinal mucosal integrity, and immune function in weaned piglets. A total of 240 weaned piglets with an average body weight (BW) of 8.82 ± 0.05 kg (28 d of age) were assigned randomly to 4 dietary treatments in a 28-d trial, including a control diet (CON), 3 diets with XOS supplementation at the concentration of 100, 500 and 1000 mg/kg (XOS100, XOS500, and XOS1000). There were 4 replicates per treatment with 15 pigs per pen. From d 1 to 14, there were no differences (P > 0.05) in average daily gain (ADG), average daily feed intake (ADFI), and gain to feed ratio (G:F) during the different treatments. The different doses of XOS showed a quadratic effect on BW on d 28, ADG and G:F d 1-28 of piglets (P < 0.05). From d 15 to 28, ADG of pigs fed the XOS500 diet was higher (P < 0.05) than pigs fed the CON diet. During the overall period (d 1 to 28), pigs fed the XOS500 diet had a higher BW, ADG and G:F than pigs fed the CON diet (P < 0.05). In addition, compared with the CON group, the XOS500 group had significantly higher serum total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD) and catalase (CAT) levels and lower malondialdehyde (MDA) levels on d 14 and 28 (P < 0.05). The serum immunoglobulin G (IgG) concentration in the XOS500 group was also significantly higher compared with the CON group on d 14 and 28 (P <0.05). However, serum immunoglobulin A (IgA) and immunoglobulin M (IgM) were not affected by the dietary treatments. Supplementation of XOS500 to the feed significantly increased the villus height (VH) and villus height to crypt depth ratio (VH:CD) in the jejunum and ileum in comparison with the CON and XOS1000 group. Moreover, the XOS500 group significantly elevated the expression levels of Occludin and zonula occludens protein-1 (ZO-1) in the ileum compared to the CON group. The ileal interleukin (IL)-1β, IL-8 and interferon (IFN)-γ mRNA expression levels in the XOS100 and XOS500 group were markedly lower than in the CON group. In contrast, the ileal IL-10 mRNA expression levels were remarkably higher in the XOS500 than CON group. In conclusion, xylo-oligosaccharides have a beneficial effect on growth performance by improving serum antioxidant defense system, serum IgG, small intestinal structure and intestinal barrier function in weaned piglets.

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Dietary chitosan-Cu chelate affects growth performance and small intestinal morphology and apoptosis in weaned piglets.

Czech Journal of Animal Science ◽

10.17221/85/2015-cjas ◽

2017 ◽

Vol 62 (No. 1) ◽

pp. 15-21

Author(s):

X. Yue ◽

L. Hu ◽

X. Fu ◽

M. Lv ◽

X. Han

Keyword(s):

Epithelial Cell ◽

Growth Performance ◽

Cell Apoptosis ◽

Intestinal Morphology ◽

Weaned Piglets ◽

Villus Height ◽

Crypt Depth ◽

Epithelial Cell Apoptosis ◽

Diarrhea Incidence ◽

Small Intestinal

The effects of dietary chitosan-copper chelate (CS-Cu) on growth performance, diarrhea, intestinal morphology and epithelial cell apoptosis in weaned piglets was investigated. One hundred and sixty Duroc × Landrace × Yorkshire weanling barrows with an average body weight of 7.75 kg were randomly assigned to one of the following dietary treatments: (1) control, (2) 100 mg Cu/kg diet from CuSO4, (3) 100 mg Cu/kg diet from CuSO4 mixed with chitosan (CuSO4+CS), (4) 100 mg Cu/kg diet from CS-Cu. The feeding trial lasted for 30 days. The results showed that the pigs receiving a diet containing CS-Cu had higher average daily gain and lower diarrhea incidence than the pigs receiving dietary CuSO4 and CuSO4+CS. Villus height and the ratio of villus height/crypt depth in duodenum, jejunum, and ileum were higher and crypt depth was lower in CS-Cu treated pigs than in pigs fed dietary CuSO4 or CuSO4+CS. An apparent decrease of ileal epithelial cell apoptosis in pigs fed CS-Cu diet was found. The activities of antioxidant enzymes were higher in pigs fed dietary CS-Cu than in those fed other diets. The results indicated that dietary CS-Cu showed better biological and physiological function in improving small intestinal morphology and reducing diarrhea incidence.

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Effect of prebiotic and synbiotic supplementation in diet on growth performance, small intestinal morphology, stress, and bacterial population under high stocking density condition of broiler chickens

Poultry Science ◽

10.3382/ps/pez152 ◽

2019 ◽

Vol 98 (10) ◽

pp. 4595-4605 ◽

Cited By ~ 2

Author(s):

Chayatid Kridtayopas ◽

Choawit Rakangtong ◽

Chaiyapoom Bunchasak ◽

Wiriya Loongyai

Keyword(s):

Growth Performance ◽

Broiler Chickens ◽

Bacterial Population ◽

Stocking Density ◽

Intestinal Morphology ◽

Density Condition ◽

Small Intestinal ◽

Synbiotic Supplementation

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A combination of xylanase, amylase and protease influences growth performance, nutrient utilisation, starch and protein digestive dynamics in broiler chickens offered maize-, sorghum- and wheat-based diets

Animal Production Science ◽

10.1071/an14657 ◽

2015 ◽

Vol 55 (10) ◽

pp. 1255 ◽

Cited By ~ 6

Author(s):

S. Y. Liu ◽

D. J. Cadogan ◽

A. Péron ◽

H. H. Truong ◽

P. H. Selle

Keyword(s):

Growth Performance ◽

Proximal Jejunum ◽

Apparent Digestibility ◽

Feed Conversion ◽

Nutrient Utilisation ◽

Enzyme Combination ◽

Metabolisable Energy ◽

Small Intestinal ◽

Enzyme Supplementation ◽

The Impact

In order to examine the influence of an enzyme combination of xylanase, amylase and protease on growth performance and energy utilisation in boiler chickens offered maize-, sorghum- and wheat-based diets and also determine the impact of exogenous enzymes on digestive dynamics of starch and protein in the small intestine and their relativity to broiler performance, a 3 × 2 factorial array of dietary treatments were offered to 288 male Ross 308 chicks from 7 to 27 days post-hatch. Apparent digestibility coefficients of starch, protein and fat in the proximal jejunum, distal jejunum, proximal ileum and distal ileum were determined at Day 27. The digestion rates of starch, protein and energy were predicted by using exponential mathematical models to fit apparent digestibility coefficients with mean retention times in each small intestinal segment. Sorghum-based diets supported the highest weight gain (P < 0.05) and feed intake (P < 0.05) but feed conversion ratios (FCR) were similar across all three grain-based diets (P > 0.10). There were significant interactions between grain type and enzyme supplementation in FCR and the enzyme combination significantly improved FCR in maize-based diets only (P < 0.01). The enzyme combination significantly increased apparent metabolisable energy (AME) in all three grain-based diets (P < 0.05) and increased nitrogen-corrected apparent metabolisable energy (AMEn) in maize- and wheat-based diets (P < 0.05). Wheat-based diets had the lowest N retention (P < 0.01), relative gizzard weight (P < 0.001) and highest gizzard pH (P < 0.05). There were significant grain × enzyme supplementation interactions for starch (P < 0.01) and N (P < 0.05) digestibility coefficients in the four small intestinal segments. Starch digestibility responses to the enzyme combination were most pronounced in wheat-based diets with significant improvement in all segments. The enzyme combination significantly retarded starch digestion rates (P < 0.05) but did not influence protein (N) digestion rates (P > 0.25). In conclusion, feed conversion of sorghum-based diet (1.475) was significantly more efficient than those based on maize (1.518) and wheat (1.532) in non-supplemented diets. The enzyme combination significantly improved energy utilisation (AME) in all three grain-based diets and improved feed conversion efficiency in maize-based diets.

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