Detection of Coxiella burnetii in Cow's Milk using the Polymerase Chain Reaction (PCR)

1994 ◽  
Vol 41 (1-10) ◽  
pp. 580-587 ◽  
Author(s):  
H. Willems ◽  
D. Thiele ◽  
R. Frölich-Ritter ◽  
H. Krauss
Keyword(s):  
Polymerase Chain Reaction ◽  
Coxiella Burnetii ◽  
Cow’S Milk ◽  
Chain Reaction ◽  
Cow's Milk ◽  
Polymerase Chain

Identification of Cow's Milk in “Buffalo” Cheese by Duplex Polymerase Chain Reaction

2002 ◽  
Vol 65 (2) ◽  
pp. 362-366 ◽  
Author(s):  
M. T. BOTTERO ◽  
T. CIVERA ◽  
A. ANASTASIO ◽  
R. M. TURI ◽  
S. ROSATI
Keyword(s):  
Polymerase Chain Reaction ◽  
Retail Trade ◽  
Cow’S Milk ◽  
Chain Reaction ◽  
Cow's Milk ◽  
Polymerase Chain ◽  
Duplex Polymerase Chain Reaction ◽  
Cheese Products ◽  
Species Specific ◽  
Single Reaction

A duplex polymerase chain reaction (PCR) was developed to identify the milk of bovine and buffalo species in cheese products, particularly in mozzarella cheese, a typical Italian cheese made from buffalo's milk. Two sets of primers were designed on the basis of the alignment of the sequence codifying mitochondrial cyt b available in the GenBank database. The primers proved to be species-specific, giving rise to 279-bp (bovine) and 192-bp (buffalo) amplified fragments. Since the amplification conditions for bovine and buffalo primers were identical, a duplex PCR was successfully applied to identify the two species in a single reaction step. This technique, when used to test cheese products from the retail trade, allowed the detection of partial or even total substitution of cow's milk for buffalo's milk, in some cases in samples of cheese misleadingly labeled “pure buffalo” mozzarella.

scholarly journals Quantitative assessment of Lactococcus lactis subsp. cremoris present in artisanal raw cow’s milk cheese

2018 ◽  
Vol 87 (2) ◽  
pp. 189-195
Author(s):  
Milena Alicja Stachelska
Keyword(s):  
Polymerase Chain Reaction ◽  
Lactococcus Lactis ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Cow’S Milk ◽  
Chain Reaction ◽  
Cow's Milk ◽  
Polymerase Chain ◽  
Cheese Production ◽  
Raw Cow’S Milk

Lactococcus lactis subsp. cremoris belongs to lactic acid bacteria that play a crucial role in cheese production and it is known to be beneficial to human health. The aim of the study was to establish a rapid and accurate quantitative real-time polymerase chain reaction (qPCR) method to detect and enumerate L. lactis subsp. cremoris in artisanal raw cow’s milk cheese. Artisanal raw cow’s milk cheese samples were used to check for presence and number of L. lactis subsp. cremoris strains. The method applies a set of target-specific PCR (polymerase chain reaction) primers and a fluorogenic probe, and amplifies a part of the LACR_RS01280 gene that encodes the aminoacetone oxidase family flavin adenine dinucleotide (FAD) binding enzyme. All 5 L. lactis subsp. cremoris strains examined were found to be qPCR positive. There was no signal recorded for 8 strains which belong to closely related species. The limit of detection amounted to ten copies per reaction and the assay indicated a linear dynamic range of seven logs. This method may be applied in detection and enumeration of L. lactis subsp. cremoris in cheese during its ripening. Moreover, it may be applied to examine the distribution of L. lactis subsp. cremoris during the cheese production and ripening.

scholarly journals Determination of Coxiella burnetii in bovine foetuses using PCR and immunohistochemistry

2017 ◽  
Vol 61 (No. 8) ◽  
pp. 421-427 ◽  
Author(s):  
M. Ozkaraca ◽  
S. Ceribasi ◽  
AO Ceribasi ◽  
A. Kilic ◽  
S. Altun ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Coxiella Burnetii ◽  
Chain Reaction ◽  
Eastern Turkey ◽  
Staining Methods ◽  
Polymerase Chain ◽  
First Time

This study was aimed at detection of Coxiella burnetii in bovine foetuses using polymerase chain reaction (PCR) and immunohistochemistry (IHC) and at an estimation of its frequency in Eastern Turkey. Stamp, Giemsa, and Gimenez stains were used in addition to PCR and IHC to determine the presence of C. burnetii in samples from 70 bovine foetuses. While the staining methods did not detect the agent by direct visualisation of C. burnetii on smears, PCR and IHC identified its presence in two of the foetuses. The distribution of antigens in these two foetuses was, in decreasing order of concentration, in the spleen, the thymus, the lungs, the liver, and the kidneys. We conclude that C. burnetii diagnosis in bovine foetuses can be reliably performed using PCR and IHC. In addition, the frequency of 1.42% of C. burnetii positivity in bovine foetuses reported here was the first time that the presence of this agent was determined in Eastern Turkey.

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