A direct hybridization approach to gene transfer from Aegilops tauschii Coss. to Triticum aestivum L

We report the results of a study on the effectiveness of Cot filtration (CF) in the characterization of the gene space of bread wheat (Triticum aestivum L.), a large genome species (1C = 16 700 Mb) of tremendous agronomic importance. Using published Cot data as a guide, 2 genomic libraries for hexaploid wheat were constructed from the single-stranded DNA collected at Cot values > 1188 and 1639 M·s. Compared with sequences from a whole genome shotgun library from Aegilops tauschii (the D genome donor of bread wheat), the CF libraries exhibited 13.7-fold enrichment in genes, 5.8-fold enrichment in unknown low-copy sequences, and a 3-fold reduction in repetitive DNA. CF is twice as efficient as methylation filtration at enriching wheat genes. This research suggests that, with improvements, CF will be a highly useful tool in sequencing the gene space of wheat.Key words: gene enrichment, renaturation kinetics, gene-rich regions, bread wheat.

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Association analysis of grain traits with SSR markers between Aegilops tauschii and hexaploid wheat (Triticum aestivum L.)

Journal of Integrative Agriculture ◽

10.1016/s2095-3119(15)61070-x ◽

2015 ◽

Vol 14 (10) ◽

pp. 1936-1948 ◽

Cited By ~ 3

Author(s):

Jing-lan ZHAO ◽

Hong-wei WANG ◽

Xiao-cun ZHANG ◽

Xu-ye DU ◽

An-fei LI ◽

...

Keyword(s):

Triticum Aestivum ◽

Ssr Markers ◽

Association Analysis ◽

Hexaploid Wheat ◽

Aegilops Tauschii ◽

Triticum Aestivum L

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Phenylalanine Ammonia-Lyase (PAL) Genes Family in Wheat (Triticum aestivum L.): Genome-Wide Characterization and Expression Profiling

Agronomy ◽

10.3390/agronomy11122511 ◽

2021 ◽

Vol 11 (12) ◽

pp. 2511

Author(s):

Fatima Rasool ◽

Muhammad Uzair ◽

Muhammad Kashif Naeem ◽

Nazia Rehman ◽

Amber Afroz ◽

...

Keyword(s):

Triticum Aestivum ◽

Phenylalanine Ammonia Lyase ◽

Chromosomal Rearrangements ◽

Aegilops Tauschii ◽

Triticum Aestivum L ◽

Regulatory Elements ◽

Phenylpropanoid Pathway ◽

Evolutionary Analysis ◽

Genome Wide ◽

A Genome

Phenylalanine ammonia-lyase (PAL) is the first enzyme in the phenylpropanoid pathway and plays a vital role in adoption, growth, and development in plants but in wheat its characterization is still not very clear. Here, we report a genome-wide identification of TaPAL genes and analysis of their transcriptional expression, duplication, and phylogeny in wheat. A total of 37 TaPAL genes that cluster into three subfamilies have been identified based on phylogenetic analysis. These TaPAL genes are distributed on 1A, 1B, 1D, 2A, 2B, 2D, 4A, 5B, 6A, 6B, and 6D chromosomes. Gene structure, conserved domain analysis, and investigation of cis-regulatory elements were systematically carried out. Chromosomal rearrangements and gene loss were observed by evolutionary analysis of the orthologs among Triticum urartu, Aegilops tauschii, and Triticum aestivum during the origin of bread wheat. Gene ontology analysis revealed that PAL genes play a role in plant growth. We also identified 27 putative miRNAs targeting 37 TaPAL genes. The high expression level of PAL genes was detected in roots of drought-tolerant genotypes compared to drought-sensitive genotypes. However, very low expressions of TaPAL10, TaPAL30, TaPAL32, TaPAL3, and TaPAL28 were recorded in all wheat genotypes. Arogenate dehydratase interacts with TaPAL29 and has higher expression in roots. The analysis of all identified genes in RNA-seq data showed that they are expressed in roots and shoots under normal and abiotic stress. Our study offers valuable data on the functioning of PAL genes in wheat.

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Genome-wide analysis of the polyamine oxidase gene family in wheat (Triticum aestivum L.) reveals involvement in temperature stress response

10.1101/2020.07.06.189209 ◽

2020 ◽

Author(s):

Fatemeh Gholizadeh ◽

Ghader Mirzaghaderi

Keyword(s):

Triticum Aestivum ◽

Abiotic Stresses ◽

Developmental Stages ◽

Expression Profiles ◽

Aegilops Tauschii ◽

Triticum Aestivum L ◽

Amine Oxidases ◽

Oxidase Gene ◽

Evolutionary Features ◽

Heat And Cold Stress

AbstractAmine oxidases (AOs) including copper containing amine oxidases (CuAOs) and FAD-dependent polyamine oxidases (PAOs) are associated with polyamine catabolism in the peroxisome, apoplast and cytoplasm and play an essential role in growth and developmental processes and response to biotic and abiotic stresses. Here, we identified PAO genes in common wheat (Triticum aestivum), T. urartu and Aegilops tauschii and reported the genome organization, evolutionary features and expression profiles of the wheat PAO genes (TaPAO). Expression analysis using publicly available RNASeq data showed that TaPAO genes are expressed redundantly in various tissues and developmental stages. A large percentage of TaPAOs respond significantly to abiotic stresses, especially temperature (i.e. heat and cold stress). Some TaPAOs were also involved in response to other stresses such as powdery mildew, stripe rust and Fusarium infection. Overall, TaPAOs may have various functions in stress tolerances responses, and play vital roles in different tissues and developmental stages. Our results provided a reference for further functional investigation of TaPAO proteins.

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Polymorphism of Got2 DNA sequences sheds light on Aegilops tauschii Coss. intraspecies divergence and origin of Triticum aestivum L.

Genetic Resources and Crop Evolution ◽

10.1007/s10722-016-0461-5 ◽

2016 ◽

Vol 64 (7) ◽

pp. 1623-1640 ◽

Cited By ~ 5

Author(s):

Alexander Ju. Dudnikov

Keyword(s):

Triticum Aestivum ◽

Dna Sequences ◽

Aegilops Tauschii ◽

Triticum Aestivum L

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Rapid development of PCR-based genome-specific repetitive DNA junction markers in wheat

Genome ◽

10.1139/g09-033 ◽

2009 ◽

Vol 52 (6) ◽

pp. 576-587 ◽

Cited By ~ 45

Author(s):

Humphrey Wanjugi ◽

Devin Coleman-Derr ◽

Naxin Huo ◽

Shahryar F. Kianian ◽

Ming-Cheng Luo ◽

...

Keyword(s):

Triticum Aestivum ◽

Transposable Elements ◽

Repetitive Dna ◽

Hexaploid Wheat ◽

Rapid Development ◽

Aegilops Tauschii ◽

Triticum Aestivum L ◽

Dynamic Evolution ◽

D Genome ◽

Repeat Dna

In hexaploid wheat ( Triticum aestivum L.) (AABBDD, C = 17 000 Mb), repeat DNA accounts for ∼90% of the genome, of which transposable elements (TEs) constitute 60%–80%. Despite the dynamic evolution of TEs, our previous study indicated that the majority of TEs are conserved and collinear between the homologous wheat genomes, based on identical insertion patterns. In this study, we exploited the unique and abundant TE insertion junction regions identified from diploid Aegilops tauschii to develop genome-specific repeat DNA junction markers (RJM) for use in hexaploid wheat. In this study, both BAC end and random shotgun sequences were used to search for RJM. Of the 300 RJM primer pairs tested, 269 (90%) amplified single bands from diploid Ae. tauschii. Of these 269 primer pairs, 260 (97%) amplified hexaploid wheat and 9 (3%) amplified Ae. tauschii only. Among the RJM primers that amplified hexaploid wheat, 88% were successfully assigned to individual chromosomes of the hexaploid D genome. Among the 38 RJM primers mapped on chromosome 6D, 31 (82%) were unambiguously mapped to delineated bins of the chromosome using various wheat deletion lines. Our results suggest that the unique RJM derived from the diploid D genome could facilitate genetic, physical, and radiation mapping of the hexaploid wheat D genome.

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