scholarly journals Isolation and characterization of unsaturated fatty acids during bacteriorhodopsin preparation from Halobacterium halobium

2007 ◽  
Vol 45 (3) ◽  
pp. 258-261 ◽  
Author(s):  
D. Li ◽  
J. Chen
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Halobacterium Halobium ◽  
Isolation And Characterization

Isolation and characterization of unsaturated fatty acids as natural ligands for the retinoid-X receptor

2003 ◽  
Vol 420 (1) ◽  
pp. 185-193 ◽  
Author(s):  
Jonathan T Goldstein ◽  
Agnieszka Dobrzyn ◽  
Margaret Clagett-Dame ◽  
J.Wesley Pike ◽  
Hector F DeLuca
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Retinoid X Receptor ◽  
Isolation And Characterization ◽  
Natural Ligands

scholarly journals Chemical characterization of effective and ineffective strains of Rhizobium leguminosarum bv. viciae.

1999 ◽  
Vol 46 (4) ◽  
pp. 1001-1009
Author(s):  
S F Izmailov ◽  
G Y Zhiznevskaya ◽  
L V Kosenko ◽  
G N Troitskaya ◽  
N N Kudryavtseva ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Rhizobium Leguminosarum ◽  
Unsaturated Fatty Acids ◽  
Effective Strain ◽  
Chemical Characterization ◽  
Unsaturation Index ◽  
Fa Composition ◽  
Sds Page ◽  
Lower Molecular Mass

Chemical composition of lipopolysaccharide (LPS) isolated from an effective (97) and ineffective (87) strains of R. l. viciae has been determined. LPS preparations from the two strains contained: glucose, galactose, mannose, fucose, arabinose, heptose, glucosamine, galactosamine, quinovosamine, and 3-N-methyl-3,6-dideoxyhexose, as well as glucuronic, galacturonic and 3-deoxyoctulosonic acid. The following fatty acids were identified: 3-OH 14:0, 3-OH 15:0, 3-OH 16:0, 3-OH 18:0 and 27-OH 28:0. The ratio of 3-OH 14:0 to other major fatty acids in LPS 87 was higher that in LPS 97. SDS/PAGE profiles of LPS indicated that, in lipopolysaccharides, relative content of S form LPS I to that of lower molecular mass (LPS II) was much higher in the effective strain 97 than in 87. All types of polysaccharides exo-, capsular-, lipo, (EPS, CPS, LPS, respectively) examined possessed the ability to bind faba bean lectin. The degree of affinity of the host lectin to LPS 87 was half that to LPS 97. Fatty acids (FA) composition from bacteroids and peribacteroid membrane (PBM) was determined. Palmitic, stearic and hexadecenoic acids were common components found in both strains. There was a high content of unsaturated fatty acids in bacteroids as well as in PBM lipids. The unsaturation index in the PBM formed by strain 87 was lower than in the case of strain 97. Higher ratio of 16:0 to 18:1 fatty acids was characteristic for PMB of the ineffective strain.

scholarly journals Characterization of the unique In Vitro effects of unsaturated fatty acids on the formation of amyloid β fibrils

PLoS ONE ◽  
2019 ◽  
Vol 14 (7) ◽  
pp. e0219465 ◽  
Author(s):  
Miki Eto ◽  
Tadafumi Hashimoto ◽  
Takao Shimizu ◽  
Takeshi Iwatsubo
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Amyloid Β ◽  
In Vitro Effects

scholarly journals Identification and Characterization of Oleaginous Yeast Isolated from Kefir and Its Ability to Accumulate Intracellular Fats in Deproteinated Potato Wastewater with Different Carbon Sources

2017 ◽  
Vol 2017 ◽  
pp. 1-19 ◽  
Author(s):  
Iwona Gientka ◽  
Marek Kieliszek ◽  
Karolina Jermacz ◽  
Stanisław Błażejak
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fossil Fuels ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Carbon Sources ◽  
Debaryomyces Hansenii ◽  
Industry Waste ◽  
Identification And Characterization

The search for efficient oleaginous microorganisms, which can be an alternative to fossil fuels and biofuels obtained from oilseed crops, has been going on for many years. The suitability of microorganisms in this regard is determined by their ability to biosynthesize lipids with preferred fatty acid profile along with the concurrent utilization of energy-rich industrial waste. In this study, we isolated, characterized, and identified kefir yeast strains using molecular biology techniques. The yeast isolates identified wereCandida inconspicua,Debaryomyces hansenii,Kluyveromyces marxianus,Kazachstania unispora, andZygotorulaspora florentina. We showed that deproteinated potato wastewater, a starch processing industry waste, supplemented with various carbon sources, including lactose and glycerol, is a suitable medium for the growth of yeast, which allows an accumulation of over 20% of lipid substances in its cells. Fatty acid composition primarily depended on the yeast strain and the carbon source used, and, based on our results, most of the strains met the criteria required for the production of biodiesel. In particular, this concerns a significant share of saturated fatty acids, such as C16:0 and C18:0, and unsaturated fatty acids, such as C18:1 and C18:2. The highest efficiency in lipid biosynthesis exceeded 6.3 g L−1.Kazachstania unisporawas able to accumulate the high amount of palmitoleic acid.

scholarly journals Substrate-specificities of acid and alkaline ceramidases in fibroblasts from patients with Farber disease and controls

1982 ◽  
Vol 205 (2) ◽  
pp. 419-425 ◽  
Author(s):  
Toru Momoi ◽  
Yoav Ben-Yoseph ◽  
Henry L. Nadler
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Specific Activity ◽  
Kinetic Studies ◽  
Significant Activity ◽  
Acid Ceramidase ◽  
Ph Optimum ◽  
Farber Disease ◽  
Normal Controls

The specific activity of acid ceramidase (N-acylsphingosine deacylase, EC 3.5.1.23) was measured at pH4.5 in normal fibroblasts and in fibroblasts from patients with Farber disease and obligate heterozygotes. Greater activity was found when the synthetically made ceramide substrates contained shorter-chain fatty acids or higher content of double bonds. Acid ceramidase activities towards N-lauroyl- (C12:0), N-myristoyl- (C14:0) and N-palmitoyl- (C16:0) sphingosine (C18:1) were respectively about 38, 26 and 6 times higher than the activity towards the N-stearoyl (C18:0) substrate. The activity towards N-linolenoylsphingosine (C18:3/C18:1), N-linoleoylsphingosine (C18:2/C18:1) and N-oleoylsphingosine (C18:1/C18:1) were respectively about 5, 4 and 3 times higher than the activity towards N-stearoylsphingosine (C18:0/C18:1). The activity towards N-stearoyldihydrosphingosine (C18:0/C18:0) was about 40% of that towards N-stearoylsphingosine. Fibroblast alkaline ceramidase possessed significant activity only towards ceramides of unsaturated fatty acids, with a pH optimum of about 9.0. Deficiency of acid ceramidase activity in fibroblasts from patients with Farber disease and intermediate activities in obligate heterozygotes were demonstrated with all ceramides examined except for N-hexanoylsphingosine (C6:0/C18:1), whereas alkaline ceramidase activity was unaffected. Comparative kinetic studies of acid ceramidase activity with N-lauroylsphingosine and N-oleoylsphingosine demonstrated about 5 (2–12)-fold and 7 (4–17)-fold higher Km values in fibroblasts from patients with Farber disease as compared with normal controls. N-Lauroylsphingosine, towards which acid ceramidase activity in control fibroblasts was about 10 times higher than that towards N-oleoylsphingosine, may serve as a better substrate for enzymic diagnosis of Farber disease as well as for further characterization of the catalytically defective acid ceramidase.

scholarly journals Synthesis and Characterization of Crosslinked Polymers from Cottonseed Oil

2018 ◽  
Author(s):  
Rangana Wijayapala ◽  
Satish Mishra ◽  
Bill Elmore ◽  
Charles Freeman Jr ◽  
Santanu Kundu
Keyword(s):  
Mechanical Properties ◽  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Tensile Modulus ◽  
Cottonseed Oil ◽  
Synthesis And Characterization ◽  
Network Polymer ◽  
Crosslinked Polymers ◽  
Alkaline Environment

In this study, crosslinked polymers were synthesized from cottonseed oil. Unsaturated fatty acids, the major components of cottonseed oils, were initially epoxidized. A network polymer was then formed by crosslinking the epoxidized oil with maleic anhydrate. Mechanical properties of these polymers were altered by varying the amount of maleic anhydrate. These polymers have a tensile modulus of the order 1 MPa and are stable in the acidic and alkaline environment.<br>

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