scholarly journals Lifespan extension by dietary restriction is not linked to protection against somatic DNA damage in Drosophila melanogaster

Aging Cell ◽  
2009 ◽  
Vol 8 (3) ◽  
pp. 331-338 ◽  
Author(s):  
Ursula Edman ◽  
Ana Maria Garcia ◽  
Rita A. Busuttil ◽  
Dylan Sorensen ◽  
Martha Lundell ◽  
...  
Keyword(s):  
Dna Damage ◽  
Drosophila Melanogaster ◽  
Dietary Restriction ◽  
Lifespan Extension

Lifespan extension by dietary restriction in female Drosophila melanogaster is not caused by a reduction in vitellogenesis or ovarian activity

2004 ◽  
Vol 39 (7) ◽  
pp. 1011-1019 ◽  
Author(s):  
William Mair ◽  
Carla M Sgrò ◽  
Alice P Johnson ◽  
Tracey Chapman ◽  
Linda Partridge
Keyword(s):  
Drosophila Melanogaster ◽  
Dietary Restriction ◽  
Lifespan Extension ◽  
Ovarian Activity

scholarly journals Testing evolutionary explanations for the lifespan benefit of dietary restriction in Drosophila melanogaster

2020 ◽  
Author(s):  
Eevi Savola ◽  
Clara Montgomery ◽  
Fergal M. Waldron ◽  
Katy Monteith ◽  
Pedro Vale ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Dietary Restriction ◽  
Side Effect ◽  
Bacterial Pathogen ◽  
Lifespan Extension ◽  
Trade Off ◽  
Scarce Resources ◽  
Evolutionary Hypothesis ◽  
Laboratory Conditions ◽  
Extended Lifespan

ABSTRACTDietary restriction (DR), limiting calories or specific nutrients, extends lifespan across diverse taxa. This lifespan extension has been explained as diet-mediated changes in the trade-off between lifespan and reproduction, with survival favoured with scarce resources. Another evolutionary hypothesis suggests the selective benefit of the response is the maintenance of reproduction. This hypothesis predicts that lifespan extension is a side effect of benign laboratory conditions, where DR individuals are frailer and unable to deal with additional stressors, and thus lifespan extension should disappear under more stressful conditions. We tested this by rearing outbred female Drosophila melanogaster on 10 different protein:carbohydrate diets. Flies were either infected with a bacterial pathogen (Pseudomonas entomophila), injured or unstressed. We monitored lifespan, fecundity and ageing measures. DR extended lifespan and reduced reproduction irrespective of injury and infection. These results do not support lifespan extension under DR being a side effect of benign laboratory conditions.

scholarly journals The relationship between longevity and diet is genotype dependent and sensitive to desiccation in Drosophila melanogaster

2020 ◽  
Vol 223 (23) ◽  
pp. jeb230185
Author(s):  
Andrew W. McCracken ◽  
Eleanor Buckle ◽  
Mirre J. P. Simons
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Variation ◽  
Dietary Restriction ◽  
Water Availability ◽  
Reaction Norms ◽  
Desiccation Stress ◽  
Lifespan Extension ◽  
Ageing Research ◽  
The Relationship ◽  
Water Supplementation

ABSTRACTDietary restriction (DR) is a key focus in ageing research. Specific conditions and genotypes were recently found to negate lifespan extension by DR, questioning its universal relevance. However, the concept of dietary reaction norms explains why the effects of DR might be obscured in some situations. We tested the importance of dietary reaction norms by measuring longevity and fecundity on five diets in five genotypes, with and without water supplementation in female Drosophila melanogaster (N>25,000). We found substantial genetic variation in the response of lifespan to diet. Flies supplemented with water rescued putative desiccation stress on the richest diets, suggesting that water availability can be an experimental confound. Fecundity declined on these richest diets, but was unaffected by water, and this reduction is thus most likely to be caused by nutritional toxicity. Our results demonstrate empirically that a range of diets need to be considered to conclude an absence of the DR longevity effect.

scholarly journals In Vivo and In Vitro Assays Evaluating the Biological Activity of Taurine, Glucose and Energetic Beverages

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2198
Author(s):  
Marcos Mateo-Fernández ◽  
Fernando Valenzuela-Gómez ◽  
Rafael Font ◽  
Mercedes Del Río-Celestino ◽  
Tania Merinas-Amo ◽  
...  
Keyword(s):  
Dna Damage ◽  
Drosophila Melanogaster ◽  
Biological Activity ◽  
Biological Effects ◽  
Methylation Status ◽  
Repetitive Sequences ◽  
Energy Drinks ◽  
Red Bull

Taurine is one of the main ingredients used in energy drinks which are highly consumed in adolescents for their sugary taste and stimulating effect. With energy drinks becoming a worldwide phenomenon, the biological effects of these beverages must be evaluated in order to fully comprehend the potential impact of these products on the health due to the fact nutrition is closely related to science since the population consumes food to prevent certain diseases. Therefore, the aim of this study was to evaluate the biological effects of taurine, glucose, classic Red Bull® and sugar-free Red Bull® in order to check the food safety and the nutraceutical potential of these compounds, characterising different endpoints: (i) Toxicology, antitoxicology, genotoxicology and life expectancy assays were performed in the Drosophila melanogaster model organism; (ii) The in vitro chemopreventive activity of testing compounds was determined by assessing their cytotoxicity, the proapoptotic DNA-damage capability to induce internucleosomal fragmentation, the strand breaks activity and the modulator role on the methylation status of genomic repetitive sequences of HL-60 promyelocytic cells. Whereas none tested compounds showed toxic or genotoxic effect, all tested compounds exerted antitoxic and antigenotoxic activity in Drosophila. Glucose, classic Red Bull® and sugar-free Red Bull® were cytotoxic in HL-60 cell line. Classic Red Bull® induced DNA internucleosomal fragmentation although none of them exhibited DNA damage on human leukaemia cells. In conclusion, the tested compounds are safe on Drosophila melanogaster and classic Red Bull® could overall possess nutraceutical potential in the in vivo and in vitro model used in this study. Besides, taurine could holistically be one of the bioactive compounds responsible for the biological activity of classic Red Bull®.

scholarly journals Testing evolutionary explanations for the lifespan benefit of dietary restriction in fruit flies ( Drosophila melanogaster )

Evolution ◽  
2020 ◽  
Author(s):  
Eevi Savola ◽  
Clara Montgomery ◽  
Fergal M. Waldron ◽  
Katy M. Monteith ◽  
Pedro Vale ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Dietary Restriction ◽  
Fruit Flies ◽  
Evolutionary Explanations
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