Note upon the Absorption of Substantive Dyes by Oxycelluloses of the Acidic Type

2008 ◽  
Vol 56 (1) ◽  
pp. 17-18 ◽  
Author(s):  
S. M. Neale ◽  
W. A. Stringfellow
Keyword(s):  
Acidic Type

The synthesis of weak acidic type hybrid monolith via thiol-ene click chemistry and its application in hydrophilic interaction chromatography

2017 ◽  
Vol 38 (9-10) ◽  
pp. 1325-1333 ◽  
Author(s):  
Jiao Zeng ◽  
Shengquan Liu ◽  
Menglin Wang ◽  
Shouzhuo Yao ◽  
Yingzhuang Chen
Keyword(s):  
Click Chemistry ◽  
Hydrophilic Interaction Chromatography ◽  
Hydrophilic Interaction ◽  
Acidic Type

Factors Affecting the Ability of a Fly Ash to Contribute to the Sulfate Resistance of Fly Ash Concrete

1988 ◽  
Vol 136 ◽  
Author(s):  
G. J. McCarthy ◽  
P. J. Tikalsky ◽  
R. L. Carrasquillo ◽  
O. E. Manz ◽  
A. Thedchanamoorthy
Keyword(s):  
Fly Ash ◽  
Calcium Aluminate ◽  
Review Paper ◽  
Sulfate Ion ◽  
Sulfate Ions ◽  
Factors Affecting ◽  
Sulfate Resistance ◽  
Fly Ash Concrete ◽  
Calcium Aluminate Hydrate ◽  
Acidic Type

ABSTRACTThe objective of this summary is to report on work in progress that is examining parameters, measurable through chemical and XRD analyses, that could indicate whether a fly ash will enhance, degrade or have no effect on the sulfate resistance of fly ash concrete.Mehta [1–4] has discussed the factors that contribute to attack of sulfates on fly ash concrete. As noted in his review paper on this subject in the preceding volume in this series [1], the agents responsible for concrete expansion and cracking are alumina-bearing hydrates, such as calcium monosulfoaluminate and calcium aluminate hydrate, that are attacked by the sulfate ion to form ettringite, calcium trisulfoaluminate. Acidic type interactions between sulfate ions and calcium hydroxide also lead to strength and mass loss.

scholarly journals Lectin affinity chromatography of glycopeptides and oligosaccharides from normal and lectin-resistant Chinese-hamster ovary cells

1982 ◽  
Vol 205 (3) ◽  
pp. 623-630 ◽  
Author(s):  
L A Hunt
Keyword(s):  
Concanavalin A ◽  
Double Mutant ◽  
Chinese Hamster Ovary Cells ◽  
Neutral Type ◽  
Chinese Hamster ◽  
Parental Cell ◽  
Ovary Cells ◽  
Lentil Lectin ◽  
Mutant Cells ◽  
Acidic Type

The [3H]mannose-labelled glycopeptides from two lectin-resistant lines of Chinese-hamster ovary cells were fractionated by chromatography on lentil lectin-Sepharose and concanavalin A-agarose columns and subsequently analysed by gel filtration in comparison with the glycopeptides of the parental cell line. Essentially all of the [3H]mannose-labelled asparaginyl-oligosaccharides from the ‘single-mutant’ cells selected for resistance to phytohaemagglutinin and the ‘double-mutant’ cells selected for additional resistance to concanavalin A were not bound to lentil lectin, whereas approximately one-sixth of the parental-cell glycopeptides were bound and specifically eluted with alpha-methyl mannoside. These bound and eluted glycopeptides represented a specific subset of the complex acidic-type asparaginyl-oligosaccharides. The percentage of radiolabelled glycopeptides and oligosaccharides from each cell line that were specifically bound to concanavalin A was consistent with the relative sensitivities of the three cell lines to this lectin. The major radiolabelled species in the endoglycosidase digest of the ‘double-mutant’-cell glycopeptides (Man4GlcNAc1-size neutral oligosaccharides) were not bound to concanavalin A, whereas essentially all of the other neutral-type oligosaccharides were bound. In addition, the larger neutral-type oligosaccharides (Man8-9GlcNAc1) were more strongly bound to concanavalin A than were either the smaller neutral-type or the di-antennary acidic-type structures.

scholarly journals Both acidic-type and neutral-type asparaginyl-oligosaccharides of host-cell glycoproteins are altered in Rous-sarcoma-virus-transformed chick-embryo fibroblasts

1985 ◽  
Vol 229 (2) ◽  
pp. 441-451 ◽  
Author(s):  
L A Hunt ◽  
S E Wright
Keyword(s):  
Chick Embryo ◽  
Rous Sarcoma Virus ◽  
Neutral Type ◽  
Gel Filtration ◽  
Transformed Cells ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Embryo Fibroblasts ◽  
Untransformed Cell ◽  
Acidic Type

In comparisons of [3H]mannose-labelled glycopeptides from chick-embryo fibroblasts infected and transformed with non-defective Prague C Rous-sarcoma virus and from untransformed fibroblasts infected with a transformation-defective derivative of Prague C Rous-sarcoma virus, we have detected transformation-dependent alterations in both the acidic-type and the neutral-type asparagine-linked oligosaccharides of cellular glycoproteins. Pronase-digested glycopeptides were analysed by the combined techniques of gel filtration, exo- and endo-glycosidase digestion and concanavalin A-agarose affinity chromatography. The transformed cell glycoproteins contained more sialic acid and were enriched for more highly branched (versus biantennary) acidic-type structures compared with the untransformed cell glycoproteins, similarly to previously reported transformation-dependent alterations. In addition, the glycopeptides from the virus-transformed cells contained several neutral-type structures that were apparently absent from the untransformed cells: small neutral-type oligosaccharides (Man3GlcNAc2) that were sensitive to endo-beta-N-acetylglucosaminidase D but resistant to endo-beta-N-acetylglucosaminidase H, and oligosaccharides with the property of ‘truncated’ precursor oligosaccharides (endoglycosidase-resistant, alpha-mannosidase-sensitive). Endoglycosidase-released oligosaccharides with the properties of hybrid-type structures were derived from the glycoproteins of both transformed and untransformed cells.

The Intermediate Filament-Related System of Higher Plant Cells Shares an Epitope with Cytokeratin 8

1991 ◽  
Vol 99 (1) ◽  
pp. 91-98
Author(s):  
JOANNE H. E. ROSS ◽  
AMANDA HUTCHINGS ◽  
GEOFFREY W. BUTCHER ◽  
E. BIRGITTE LANE ◽  
CLIVE W. LLOYD
Keyword(s):  
Monoclonal Antibody ◽  
Intermediate Filament ◽  
Higher Plants ◽  
Type I ◽  
Higher Plant ◽  
Related System ◽  
Differentiated Cells ◽  
Epithelial Cell Lines ◽  
Two Kingdoms ◽  
Acidic Type

It is now apparent that plants possess intermediate filament (IF) antigens that exist either as cytoplasmic fibrillar bundles (FBs) or in a finer form, co-distributing with the microtubule arrays. In a previous study a monoclonal antibody (AFB) against the fibrillar bundles was shown to recognize representatives of what may be considered as a ‘core’ group of type III IFs. Here a monoclonal antibody is described that extends the range of similarity to cytokeratin. MAC322 stains the cytokeratin network in PtK2 cells, unlike AFB that stained vimentin. Like other anti-EF antibodies successfully used against plants, it stains their microtubule-associated system but is also able to stain FBs, with or without methanol pretreatment. By immunoblotting, MAC322 does not recognize the acidic (type I) cytokeratdns in MCF7 and TR146 epithelial cell lines but recognizes cytokeratin 8. This was confirmed by a positive cross-reaction against recombinant human cytokeratin 8. This type II polypeptide is known to be characteristic of simple epithelia, to occur in the earliest stages of amphibian development, and also in some non-epithelial and de-differentiated cells. The sharing of a cytokeratin 8 epitope between higher plants and animals reinforces the idea that IF forms evolved before the two kingdoms diverged.

Nutritional factors influencing the distribution of the calcareous and acidic populations in Hypericum perforatum

1969 ◽  
Vol 47 (1) ◽  
pp. 175-181 ◽  
Author(s):  
P. S. Ramakrishnan
Keyword(s):  
Hypericum Perforatum ◽  
Growth Yield ◽  
Good Growth ◽  
Severe Toxicity ◽  
Phosphorus Level ◽  
Natural Habitats ◽  
Wide Range ◽  
Calcium And Phosphorus ◽  
Two Populations ◽  
Acidic Type

Hypericum perforatum L. was found to have a wide range of distribution occurring on both calcareous and acidic sites. The two populations showed differential responses to macronutrients like calcium and phosphorus and micronutrients like aluminum and manganese. The acidic population showed better growth yield than the calcareous population at lower calcium levels in the medium, but at higher levels the reverse was true. The acidic population had higher uptake of calcium and phosphorus in all the different treatments and of magnesium, potassium, and sodium at low-calcium levels. The individuals of the calcareous population exhibited ‘phosphate toxicity’ at high phosphorus level in the medium while those of the acidic type gave better yield with increase in phosphorus level. Phosphorus uptake was consistently higher in the case of the acidic type compared to the calcareous population. The calcareous population was adversely affected at higher levels of aluminum and manganese and the individuals of this population exhibited severe toxicity symptoms. The acidic population gave good growth yield at both low and high levels of aluminum and manganese. The uptake of aluminum and manganese increased at higher levels of these nutrients in the media. These observations on the two populations have been discussed and it was concluded that besides the direct role of calcium, the differential response to phosphorus, aluminum, and manganese, the availability of which is determined by the pH of the soil, also control the restriction of the two ecotypes to their respective natural habitats.

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