Coxiella burnetii infection among blood donors during the 2009 Q-fever outbreak in the Netherlands

Transfusion ◽  
2011 ◽  
Vol 52 (1) ◽  
pp. 144-150 ◽  
Author(s):  
Boris M. Hogema ◽  
Ed Slot ◽  
Michel Molier ◽  
Peter M. Schneeberger ◽  
Mirjam H. Hermans ◽  
...  
Keyword(s):  
The Netherlands ◽  
Coxiella Burnetii ◽  
Blood Donors ◽  
Q Fever

scholarly journals Assessment of Coxiella burnetii presence after tick bite in north-eastern Poland

Infection ◽  
2019 ◽  
Vol 48 (1) ◽  
pp. 85-90
Author(s):  
Karol Borawski ◽  
Justyna Dunaj ◽  
Piotr Czupryna ◽  
Sławomir Pancewicz ◽  
Renata Świerzbińska ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Blood Donors ◽  
Q Fever ◽  
Phase 1 ◽  
Molecular Study ◽  
Tick Bite ◽  
Control Group ◽  
Tick Borne Encephalitis ◽  
North Eastern ◽  
History Of

Abstract Purpose The aim of the study is to assess anti-Coxiella burnetii antibodies presence in inhabitants of north-eastern Poland, to assess the risk of Q fever after tick bite and to assess the percentage of co-infection with other pathogens. Methods The serological study included 164 foresters and farmers with a history of tick bite. The molecular study included 540 patients, hospitalized because of various symptoms after tick bite. The control group consisted of 20 honorary blood donors. Anti-Coxiella burnetii antibodies titers were determined by Coxiella burnetii (Q fever) Phase 1 IgG ELISA (DRG International Inc. USA). PCR was performed to detect DNA of C. burnetii, Borrelia burgdorferi and Anaplasma phagocytophilum. Results Anti-C. burnetii IgG was detected in six foresters (7.3%). All foresters with the anti-C. burnetii IgG presence were positive toward anti-B. burgdorferi IgG and anti-TBE (tick-borne encephalitis). Anti-C. burnetii IgG was detected in five farmers (6%). Four farmers with anti-C. burnetii IgG presence were positive toward anti-B. burgdorferi IgG and two with anti-TBE. Among them one was co-infected with B. burgdorferi and TBEV. Correlations between anti-C. burnetii IgG and anti-B. burgdorferi IgG presence and between anti-C. burnetii IgG presence and symptoms of Lyme disease were observed. C. burnetii DNA was not detected in any of the 540 (0%) patients. Conclusions C. burnetii is rarely transmitted by ticks, but we proved that it is present in the environment, so it may be a danger to humans. The most common co-occurrence after tick bite concerns C. burnetii and B. burgdorferi.

scholarly journals Detection of Coxiella burnetii DNA in the environment during and after a large Q fever epidemic in the Netherlands

2013 ◽  
Vol 114 (5) ◽  
pp. 1395-1404 ◽  
Author(s):  
A. de Bruin ◽  
I. Janse ◽  
M. Koning ◽  
L. de Heer ◽  
R.Q.J. van der Plaats ◽  
...  
Keyword(s):  
The Netherlands ◽  
Coxiella Burnetii ◽  
Q Fever

scholarly journals Genotypic Diversity of Coxiella burnetii in the 2007-2010 Q Fever Outbreak Episodes in The Netherlands

2011 ◽  
Vol 50 (3) ◽  
pp. 1076-1078 ◽  
Author(s):  
J. J. H. C. Tilburg ◽  
J. W. A. Rossen ◽  
E. J. van Hannen ◽  
W. J. G. Melchers ◽  
M. H. A. Hermans ◽  
...  
Keyword(s):  
The Netherlands ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Genotypic Diversity

scholarly journals Detection of Coxiella burnetii in Complex Matrices by Using Multiplex Quantitative PCR during a Major Q Fever Outbreak in The Netherlands

2011 ◽  
Vol 77 (18) ◽  
pp. 6516-6523 ◽  
Author(s):  
A. de Bruin ◽  
A. de Groot ◽  
L. de Heer ◽  
J. Bok ◽  
P. R. Wielinga ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
The Netherlands ◽  
Quantitative Pcr ◽  
Coxiella Burnetii ◽  
Internal Control ◽  
Q Fever ◽  
Qpcr Assay ◽  
Environmental Matrices ◽  
Content Type ◽  
Control Target

ABSTRACTQ fever, caused byCoxiella burnetii, is a zoonosis with a worldwide distribution. A large rural area in the southeast of the Netherlands was heavily affected by Q fever between 2007 and 2009. This initiated the development of a robust and internally controlled multiplex quantitative PCR (qPCR) assay for the detection ofC. burnetiiDNA in veterinary and environmental matrices on suspected Q fever-affected farms. The qPCR detects threeC. burnetiitargets (icd,com1, and IS1111) and oneBacillus thuringiensisinternal control target (cry1b).Bacillus thuringiensisspores were added to samples to control both DNA extraction and PCR amplification. The performance of the qPCR assay was investigated and showed a high efficiency; a limit of detection of 13.0, 10.6, and 10.4 copies per reaction for the targetsicd,com1, and IS1111, respectively; and no cross-reactivity with the nontarget organisms tested. Screening forC. burnetiiDNA on 29 suspected Q fever-affected farms during the Q fever epidemic in 2008 showed that swabs from dust-accumulating surfaces contained higher levels ofC. burnetiiDNA than vaginal swabs from goats or sheep. PCR inhibition by coextracted substances was observed in some environmental samples, and 10- or 100-fold dilutions of samples were sufficient to obtain interpretable signals for both theC. burnetiitargets and the internal control. The inclusion of an internal control target and threeC. burnetiitargets in one multiplex qPCR assay showed that complex veterinary and environmental matrices can be screened reliably for the presence ofC. burnetiiDNA during an outbreak.

scholarly journals Screening of post-mortem tissue donors for Coxiella burnetii infection after large outbreaks of Q fever in The Netherlands

2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Marja J van Wijk ◽  
D Willemijn Maas ◽  
Nicole HM Renders ◽  
Mirjam HA Hermans ◽  
Hans L Zaaijer ◽  
...  
Keyword(s):  
The Netherlands ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Post Mortem ◽  
Tissue Donors

scholarly journals Antibodies against Coxiella burnetii and pregnancy outcome during the 2007-2008 Q fever outbreaks in the Netherlands

2011 ◽  
Vol 11 (1) ◽  
Author(s):  
Wim van der Hoek ◽  
Jamie CE Meekelenkamp ◽  
Alexander CAP Leenders ◽  
Nancy Wijers ◽  
Daan W Notermans ◽  
...  
Keyword(s):  
The Netherlands ◽  
Pregnancy Outcome ◽  
Coxiella Burnetii ◽  
Q Fever

Screening for Coxiella burnetii seroprevalence in chronic Q fever high-risk groups reveals the magnitude of the Dutch Q fever outbreak

2012 ◽  
Vol 141 (4) ◽  
pp. 847-851 ◽  
Author(s):  
L. M. KAMPSCHREUR ◽  
J. C. J. P. HAGENAARS ◽  
C. C. H. WIELDERS ◽  
P. ELSMAN ◽  
P. J. LESTRADE ◽  
...  
Keyword(s):  
High Risk ◽  
The Netherlands ◽  
Confidence Interval ◽  
Coxiella Burnetii ◽  
Catchment Area ◽  
Q Fever ◽  
Risk Groups ◽  
Chronic Q Fever ◽  
Epidemic Area ◽  
Acute Q Fever

SUMMARYThe Netherlands experienced an unprecedented outbreak of Q fever between 2007 and 2010. The Jeroen Bosch Hospital (JBH) in 's-Hertogenbosch is located in the centre of the epidemic area. Based on Q fever screening programmes, seroprevalence of IgG phase II antibodies to Coxiella burnetii in the JBH catchment area was 10·7% [785 tested, 84 seropositive, 95% confidence interval (CI) 8·5–12·9]. Seroprevalence appeared not to be influenced by age, gender or area of residence. Extrapolating these data, an estimated 40 600 persons (95% CI 32 200–48 900) in the JBH catchment area have been infected by C. burnetii and are, therefore, potentially at risk for chronic Q fever. This figure by far exceeds the nationwide number of notified symptomatic acute Q fever patients and illustrates the magnitude of the Dutch Q fever outbreak. Clinicians in epidemic Q fever areas should be alert for chronic Q fever, even if no acute Q fever is reported.

The genome of Coxiella burnetii Z3055, a clone linked to the Netherlands Q fever outbreaks, provides evidence for the role of drift in the emergence of epidemic clones

2014 ◽  
Vol 37 (5-6) ◽  
pp. 281-288 ◽  
Author(s):  
Felicetta D’Amato ◽  
Laetitia Rouli ◽  
Sophie Edouard ◽  
Judith Tyczka ◽  
Matthieu Million ◽  
...  
Keyword(s):  
The Netherlands ◽  
Coxiella Burnetii ◽  
Q Fever

scholarly journals Detection of Coxiella burnetii DNA on Small-Ruminant Farms during a Q Fever Outbreak in the Netherlands

2012 ◽  
Vol 78 (6) ◽  
pp. 1652-1657 ◽  
Author(s):  
A. de Bruin ◽  
R. Q. J. van der Plaats ◽  
L. de Heer ◽  
R. Paauwe ◽  
B. Schimmer ◽  
...  
Keyword(s):  
The Netherlands ◽  
Surface Area ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Monitoring Program ◽  
Primary Source ◽  
Dairy Goat ◽  
Environmental Matrices ◽  
Content Type ◽  
Bulk Milk

ABSTRACTDuring large Q fever outbreaks in the Netherlands between 2007 and 2010, dairy goat farms were implicated as the primary source of human Q fever. The transmission ofCoxiella burnetiito humans is thought to occur primarily via aerosols, although available data onC. burnetiiin aerosols and other environmental matrices are limited. During the outbreak of 2009, 19 dairy goat farms and one dairy sheep farm were selected nationwide to investigate the presence ofC. burnetiiDNA in vaginal swabs, manure, surface area swabs, milk unit filters, and aerosols. Four of these farms had a positive status during theCoxiella burnetiibulk milk monitoring program in 2009 and additionally reported abortion waves in 2008 or 2009. Eleven farms were reported as having positive bulk milk only, and five selected (control) farms had a bulk milk-negative status in 2009 and no reported Q fever history. Screening by quantitative PCR (qPCR) revealed that on farms with a history of abortions related toC. burnetiiand, to a lesser extent, on farms positive by bulk milk monitoring, generally higher proportions of positive samples and higher levels ofC. burnetiiDNA within positive samples were observed than on the control farms. The relatively high levels ofC. burnetiiDNA in surface area swabs and aerosols sampled in stables of bulk milk-positive farms, including farms with a Q fever-related abortion history, support the hypothesis that these farms can pose a risk for the transmission ofC. burnetiito humans.

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