scholarly journals Comparison of the broth microdilution (BMD) method of the European Committee on Antimicrobial Susceptibility Testing and the Clinical Laboratory Standards Institute BMD method for non-Candida albicans and non-C. tropicalis bloodstream isolates from eleven

2012 ◽  
Vol 12 (8) ◽  
pp. 890-896 ◽  
Author(s):  
Sonia U. Purisco ◽  
Marilena A. Martins ◽  
Maria W. Szeszs ◽  
Dulcilena M. Castro e Silva ◽  
Sandra R.B.S. Pukinskas ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Clinical Laboratory ◽  
Antimicrobial Susceptibility Testing ◽  
Broth Microdilution ◽  
European Committee

scholarly journals A survey of methods used for antimicrobial susceptibility testing in veterinary diagnostic laboratories in the United States

2017 ◽  
Vol 29 (5) ◽  
pp. 669-675 ◽  
Author(s):  
David A. Dargatz ◽  
Matthew M. Erdman ◽  
Beth Harris
Keyword(s):  
United States ◽  
Antimicrobial Resistance ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Clinical Laboratory ◽  
The United States ◽  
Antimicrobial Susceptibility Testing ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Broth Microdilution

Antimicrobial resistance is a serious threat to animal and human health worldwide, requiring a collaborative, holistic approach. The U.S. Government has developed a national strategy to address antimicrobial resistance, with one component being to monitor antimicrobial resistance in agricultural settings. We developed a survey to collect information about antimicrobial susceptibility testing (AST) from the veterinary diagnostic laboratory community in the United States, assessing current practices and technologies and determining how AST information is shared. Of the 132 surveys administered, 52 (39%) were returned. Overall, responding laboratories conducted susceptibility tests on 98,788 bacterial isolates in 2014, with Escherichia coli being the most common pathogen tested across all animal species. The 2 most common AST methods employed were the disk diffusion method (71%) and the Sensititre platform broth microdilution system (59%). Laboratories primarily used the Clinical Laboratory Standards Institute (CLSI) VET-01 standard (69%) and the automatically calculated interpretations provided by the commercial AST systems (61%) for interpreting their AST data. Only 22% of laboratories published AST data on a periodic basis, usually via annual reports published on the laboratory’s website or through peer-reviewed journals for specific pathogens. Our results confirm that disk diffusion and broth microdilution remain the standard AST methods employed by U.S. veterinary diagnostic laboratories, and that CLSI standards are commonly used for interpreting AST results. This information will help determine the most efficient standardized methodology for future surveillance. Furthermore, the current infrastructure within laboratories, once harmonized, will help provide a mechanism for conducting national surveillance programs.

scholarly journals Frequency of Voriconazole ResistanceIn Vitroamong Spanish Clinical Isolates ofCandidaspp. According to Breakpoints Established by the Antifungal Subcommittee of the European Committee on Antimicrobial Susceptibility Testing

2011 ◽  
Vol 55 (4) ◽  
pp. 1794-1797 ◽  
Author(s):  
M. Cuenca-Estrella ◽  
A. Gomez-Lopez ◽  
I. Cuesta ◽  
O. Zaragoza ◽  
E. Mellado ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antimicrobial Susceptibility ◽  
Candida Glabrata ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Clinical Isolates ◽  
Significant Percentage ◽  
European Committee ◽  
Resistant Strains ◽  
High Mics

ABSTRACTA total of 4,226 Spanish clinical isolates ofCandidaspp. were analyzed to assess resistance to voriconazole according to breakpoints established by the European Committee for Antimicrobial Susceptibility Testing (where susceptibility [S] to voriconazole corresponds to a MIC of ≤0.12 mg/liter). Resistance was uncommon amongCandida albicans(5%),C. parapsilosis(1.2%), andC. tropicalis(11%) isolates. Voriconazole MICs of >0.12 mg/liter were more frequent amongCandida glabrataandC. kruseiisolates. A significant percentage of voriconazole-resistant strains came from oropharyngeal infections and exhibited high MICs of other azoles.

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