scholarly journals Influence of the growth rate on the macromolecular composition ofAcinetobacter calcoaceticusin carbon-limited chemostat culture

1984 ◽  
Vol 23 (1) ◽  
pp. 71-75 ◽  
Author(s):  
J.C. Preez ◽  
P.M. Lategan ◽  
D.F. Toerien
Keyword(s):  
Growth Rate ◽  
Chemostat Culture ◽  
Macromolecular Composition

Effect of growth rate on the eicosapentaenoic acid and docosahexaenoic acid content of Isochrysis galbana in chemostat culture

1994 ◽  
Vol 41 (1) ◽  
pp. 23-27 ◽  
Author(s):  
E. Molina Grima ◽  
J. A. S�nchez P�rez ◽  
F. Garc�a Camacho ◽  
J. M. Fern�ndez Sevilla ◽  
F. G. Aci�n Fern�ndez
Keyword(s):  
Growth Rate ◽  
Docosahexaenoic Acid ◽  
Eicosapentaenoic Acid ◽  
Acid Content ◽  
Chemostat Culture ◽  
Isochrysis Galbana

Sebatian Metoksiaromatik Sebagai Substrat Pertumbuhan dan Pengaruh Enzim Demetilase dalam Acetobacterium Woodii DSM 1030

2012 ◽  
Author(s):  
Mohd. Sahaid Hj. Kalil ◽  
Muhammad Zaki ◽  
Wan Mohtar Wan Yusoff ◽  
Mohammad Ramlan Mohd. Salleh
Keyword(s):  
Growth Rate ◽  
Steady State ◽  
Sole Carbon Source ◽  
Specific Growth ◽  
Chemostat Culture ◽  
Carbon Sources ◽  
Syringic Acid ◽  
Organic Substrate ◽  
Acetobacterium Woodii ◽  
Vanilic Acid

Penyelidikan ini bertujuan untuk menyaring substrat organik bagi untuk penghasilan sel–sel A. woodii teraruh demetilase. Pertumbuhan A. woodii dilakukan dalam medium “Balch” yang mengandungi sumber karbon berbeza dalam keadaan anaerobik. Sebanyak sebelas substrat telah diuji iaitu anisol, 2– dan 3–metoksifenol, asid vanilik, asid siringik, asid 2,3,4–, 2,4,5– dan 3,4,5–trimetoksi benzoik, 2,3,4–, 2,4,5– dan 3,4,5–trimetoksi benzil alkohol. 2–metoksifenol merupakan substrat terbaik untuk pertumbuhan A. woodii pada kadar pertumbuhan spesifik 0.14 j–1. Penghasilan sel–sel teraruh demetilase dilakukan dalam kultur kemostat pada kadar pencairan (D) 0.0j–1. Sel-sel pada keadaan mantap dituai dalam keadaan anaerobik dan dipekatkan sebelum digunakan. Pertumbuhan A. woodii didapati maksimum dengan menggunakan kepekatan 0.62 g/L 2–metoksifenol sebagai sumber karbon tunggal. Tindak balas penyahmetilan oleh sel–sel A. woodii meningkat sebanyak 78% apabila 2–metoksifenol sebanyak 0.31 g/L ditambah dalam medium yang mengandungi fruktosa (1% w/v) semasa kultur kemostat. Kata kunci: tindak balas penyahmetilan; demetilase; sel-sel tertuai; metosiaromatik, Acetobacteriumwoodii The objective of this project was to screen organic substrate suitable for the growth of A. woodii, and as for the production of demethylase. A. woodii was grown in “Balch” medium containing different carbon sources. Eleven substrates were tested including anisole, 2– and 3–methoxyphenol, vanilic acid, syringic acid, 2,3,4–, 2,4,5– and 3,4,5–trimethoxy benzoic acid and 2,3,4–, 2,4,5– and 3,4,5–trimethoxy benzyl alcohol. It was found that 2–methoxyphenol was the best substrate with a specific growth rate of 0.14 h–1. The production of demethylase induced cells was carried out in a chemostat culture at a dilution rate (D) of 0.08 h–1. Cells were harvested at steady state of growth and concentrated before use. Optimal concentration of 2–methoxvphenol as the sole carbon source was 0.62 g/L. Demethylation reaction of 0.31 g/L 2–methoxyphenol by induced culture increases 78% relative to the chemostat culture containing only fructose. Key words: Demethylation reaction; demethylase; harvested cells; methoxyaromatic; Acetobacteriumwoodii

Effect of growth rate on the eicosapentaenoic acid and docosahexaenoic acid content of Isochrysis galbana in chemostat culture

1994 ◽  
Vol 41 (1) ◽  
pp. 23-27 ◽  
Author(s):  
E. Molina Grima ◽  
J. A. S�nchez P�rez ◽  
F. Garc�a Camacho ◽  
J. M. Fern�ndez Sevilla ◽  
F. G. Aci�n Fern�ndez
Keyword(s):  
Growth Rate ◽  
Docosahexaenoic Acid ◽  
Eicosapentaenoic Acid ◽  
Acid Content ◽  
Chemostat Culture ◽  
Isochrysis Galbana

scholarly journals Production of prodigiosin pigment by Serratia marcescens is negatively associated with cellular ATP levels during high-rate, low-cell-density growth

2020 ◽  
Vol 66 (3) ◽  
pp. 243-255 ◽  
Author(s):  
Pryce L. Haddix ◽  
Robert M.Q. Shanks
Keyword(s):  
Growth Rate ◽  
Serratia Marcescens ◽  
Cell Density ◽  
Chemostat Culture ◽  
High Rate ◽  
Phase Cell ◽  
Intact Cells ◽  
Atp Levels ◽  
Density Growth ◽  
Low Cell Density

Serratia marcescens is a facultatively anaerobic bacterium and the most recognized producer of the hydrophobic pigment prodigiosin. Previous work has shown that prodigiosin both increases ATP production during population lag phase and approximately doubles the stationary-phase cell yield. Here, we employed both batch and chemostat culture methods to investigate prodigiosin’s role during high rate growth at low cell density as peak cellular ATP levels decline. Batch culture experiments utilizing artificial pigment induction showed an ATP reduction during low cell density growth. In addition, pigment induction during fixed growth rate chemostat culture revealed a negative correlation between cellular levels of prodigiosin and ATP (r = −0.95). Variable growth rate chemostat experiments showed an inverse relationship between ATP per cell and prodigiosin per cell during low-density growth but a direct relationship during high-density growth. Rate modeling of chemostat data quantified the pigment’s effect on cellular levels of ATP for both population growth phases. Finally, prodigiosin production in a heterologous bacterium led to ATP decline. These data with intact cells complement the established in vitro proton import function of prodigiosin pigment and may indicate an energy-spilling function during high rate, low cell density growth.

Siderophore production by Fusarium venenatum A3/5

2002 ◽  
Vol 30 (4) ◽  
pp. 696-698 ◽  
Author(s):  
M. G. Wiebe
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Environmental Conditions ◽  
Continuous Flow ◽  
Specific Growth ◽  
Chemostat Culture ◽  
Siderophore Production ◽  
Batch Cultures ◽  
Chemostat Cultures ◽  
Fusarium Venenatum

Fusarium venenatum A3/5 was grown in iron-restricted batch cultures and iron-limited chemostat cultures to determine how environmental conditions affected siderophore production. The specific growth rate in iron-restricted batch cultures was 0.22 h−1, which was reduced to 0.12 h−1 when no iron was added to the culture. Derit in iron-limited chemostat culture was 0.1 h−1. Siderophore production was correlated with specific growth rate, with the highest siderophore production occurring at D = 0.08 h−1 and the lowest at D = 0.03 h−1. Siderophore production was greatest at pH 4.7 and was significantly reduced at pHs above 6.0. Siderophore production could be enhanced by providing insoluble iron instead of soluble iron in continuous flow cultures.

Regulation of respiratory activity by oxygen in Arthrobacter fluorescens ammonium-limited chemostat culture

1985 ◽  
Vol 31 (10) ◽  
pp. 896-899 ◽  
Author(s):  
I. Cacciari ◽  
D. Lippi ◽  
S. Ippoliti ◽  
T. Pietrosanti ◽  
W. Pietrosanti
Keyword(s):  
Growth Rate ◽  
Dissolved Oxygen ◽  
Respiratory Rate ◽  
Oxygen Concentration ◽  
Respiration Rate ◽  
Inverse Relationship ◽  
Respiratory Activity ◽  
Chemostat Culture ◽  
Oxygen Limitation ◽  
Dissolved Oxygen Concentration

The effect of a sudden imposition of either oxygen limitation or over oxygenation on an Arthrobacter sp. ammonium-limited chemostat culture was studied at growth rate of 0.03 h−1. Growth and respiratory activity of the cultures were examined during the transition periods and at both steady-states. Arthrobacter fluorescens responded to oxygen limitation by increasing respiratory rate and degrading polysaccharide content. In contrast, an increase in dissolved oxygen concentration caused a decrease in respiration rate and an increase in biosynthesis of endogenous polysaccharides. Under both conditions an inverse relationship seemed to occur between respiration rate and yield coefficients.

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