scholarly journals Diversity ofPseudomonasstrains isolated with King's B and Gould's S1 agar determined by repetitive extragenic palindromic-polymerase chain reaction, 16S rDNA sequencing and Fourier transform infrared spectroscopy characterisation

1999 ◽  
Vol 173 (1) ◽  
pp. 155-162 ◽  
Author(s):  
Kaare Johnsen ◽  
Preben Nielsen
Keyword(s):  
Polymerase Chain Reaction ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
16S Rdna ◽  
Fourier Transform Infrared Spectroscopy ◽  
16S Rdna Sequencing ◽  
Chain Reaction ◽  
Rdna Sequencing ◽  
Polymerase Chain ◽  
Repetitive Extragenic Palindromic

scholarly journals Detection of Arcobacter spp. in Mytilus galloprovincialis samples collected from Apulia region

2015 ◽  
Vol 4 (1) ◽  
Author(s):  
Elisabetta Bonerba ◽  
Anna Mottola ◽  
Antonio Parisi ◽  
Angela Di Pinto ◽  
Andrea Serraino ◽  
...  
Keyword(s):  
Mytilus Galloprovincialis ◽  
Disease Outbreak ◽  
16S Rdna Sequencing ◽  
Emerging Pathogen ◽  
Chain Reaction ◽  
Selective Enrichment ◽  
Apulia Region ◽  
Rdna Sequencing ◽  
Arcobacter Butzleri ◽  
Polymerase Chain

The aim of the study was to evaluate the occurrence of <em>Arcobacter</em> spp. in 20 samples of <em>Mytilus galloprovincialis</em> purchased at fish markets in Apulia region. The detection of <em>Arcobacter</em> spp. was performed, after selective enrichment, on modified charcoal cefoperazone deoxycholate (mCCD) agar supplemented with Cefoperazone, Amphotericin B and Teicoplanin (CAT). In 6 out of the 20 tested samples the presence of <em>Arcobacter</em> spp. was found and confirmed by genus-based polymerase chain reaction. All the isolates were identified as belonging to the species<em> Arcobacter butzleri</em> using 16S rDNA sequencing and BLAST online. The results represent the first report in Italy of <em>A. butzleri</em> detection in marketed <em>Mytilus galloprovincialis</em>. The survey underlines the epidemiological importance of <em>A. butzleri</em> as an emerging pathogen, and highlights that mussels should be considered as a potential cause of foodborne disease outbreak.

Molecular Typing ofMycobacterium chelonaeIsolates From a Pseudo-outbreak Involving an Automated Bronchoscope Washer

2008 ◽  
Vol 29 (11) ◽  
pp. 1088-1090 ◽  
Author(s):  
Alexandra Chroneou ◽  
Sarah K. Zimmerman ◽  
Steven Cook ◽  
Sandra Willey ◽  
Jane Eyre-Kelly ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Bronchoalveolar Lavage ◽  
Molecular Typing ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Clinical Findings ◽  
Chain Reaction ◽  
Mycobacterium Chelonae ◽  
Polymerase Chain ◽  
Repetitive Extragenic Palindromic

We describe a pseudo-outbreak ofMycobacterium chelonaeinfection in bronchoalveolar lavage fluid from 9 patients that was traced to contamination of an automated bronchoscope washer. Molecular typing using repetitive extragenic palindromic polymerase chain reaction was helpful in confirming epidemiologic and clinical findings.

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