Effect of an extract of the mushroom Agaricus blazei Murill on expression of adhesion molecules and production of reactive oxygen species in monocytes and granulocytes in human whole blood ex vivo

Aim: The primary aim of this study was to develop biomimetic nanocarriers for specific homologous targeting of the anticancer drugs ammonium pyrrolidine dithiocarbamate (PDTC) and doxorubicin. Methods: Membranous nanovesicles were synthesized from a breast cancer cell line (MCF7) by syringe extrusion process and were loaded with PDTC and doxorubicin. Besides their abilities for self-homing, the drug loaded nanovesicles showed anti-cell proliferative effects via the generation of reactive oxygen species. Results: The nanovesicles demonstrated efficient internalization via homologous targeting. Delivery of PDTC showed a higher killing effect for homologous cell targeting than other cell types. Experimental results demonstrated increased antiproliferative potency of PDTC, which induced apoptosis via reactive oxygen species generation. Conclusion: The developed membrane-derived nanocarrier is an attractive biocompatible system for ex vivo targeted drug delivery.

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Efficacy of α-tocopherol administration in community-acquired pneumonia

Russian Pulmonology ◽

10.18093/0869-0189-2007-0-3-99-102 ◽

2007 ◽

pp. 99-102

Author(s):

F. R. Farkhutdinov

Keyword(s):

Reactive Oxygen Species ◽

Whole Blood ◽

Blood Concentration ◽

Conventional Treatment ◽

Clinical Course ◽

Reactive Oxygen ◽

Redox Status ◽

Community Acquired Pneumonia ◽

Oxygen Species ◽

Laboratory Parameters

We studied effect of α tocopherol on clinical course and production of reactive oxygen species (ROS) in the whole blood in patients with community acquired pneumonia (CAP). The trial involved 70 patients with CAP. Generation of ROS was studied using the luminol dependent chemilumines cence (LDCL) method. Conventional treatment was given to all the patients. Besides this, 35 patients received α tocopherol. LDCL intensity of the blood was enhanced in all the patients. Treatment with α-tocopherol decreased ROS blood concentration and resulted in positive dynamics of clini cal and laboratory parameters. By contrast, patients on the conventional treatment maintained high LDCL intensity and there was slowly resolved course of inflammation in many cases. So, α tocopherol improved redox status in patients with CAP and increased efficiency of the treatment.

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The Effect of Physical Training on Peripheral Blood Mononuclear Cell Ex Vivo Proliferation, Differentiation, Activity, and Reactive Oxygen Species Production in Racehorses

Antioxidants ◽

10.3390/antiox9111155 ◽

2020 ◽

Vol 9 (11) ◽

pp. 1155

Author(s):

Olga Witkowska-Piłaszewicz ◽

Rafał Pingwara ◽

Anna Winnicka

Keyword(s):

Reactive Oxygen Species ◽

Cell Proliferation ◽

Immune Cell ◽

Ex Vivo ◽

Exercise Physiology ◽

Reactive Oxygen ◽

Oxygen Species ◽

Peripheral Blood Mononuclear ◽

Before And After ◽

Anti Inflammatory

Physical activity has an influence on a variety of processes in an athlete’s organism including the immune system. Unfortunately, there is a lack of studies regarding racehorse immune cells, especially when the horse model is compared to human exercise physiology. The aim of the study was to determine changes in immune cell proliferation, lymphocyte populations, and monocyte functionality in trained and untrained racehorses after exercise. In this study, field data were collected. The cells from 28 racehorses (14 untrained and 14 well-trained) were collected before and after exercise (800 m at a speed of about 800 m/min) and cultured for 4 days. The expression of CD4, CD8, FoxP3, CD14, MHCII, and CD5 in PBMC, and reactive oxygen species (ROS) production, as well as cell proliferation, were evaluated by flow cytometry. In addition, IL-1β, IL-4, IL-6, IL-10, IL-17, INF-γ, and TNF-α concentrations were evaluated by ELISA. The creation of an anti-inflammatory environment in well-trained horses was confirmed. In contrast, a pro-inflammatory reaction occurred in untrained horses after training. In conclusion, an anti-inflammatory state occurs in well-trained racehorses, which is an adaptational reaction to an increased workload during training.

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