Regeneration of oxytalan fibres in different types of periodontal defects: a histological study in monkeys

2010 ◽  
Vol 33 (8) ◽  
pp. 453-459 ◽  
Author(s):  
A. Sculean ◽  
N. Donos ◽  
E. Reich ◽  
T. Karring ◽  
M. Brecx
2003 ◽  
Vol 18 (3) ◽  
pp. 189-195 ◽  
Author(s):  
Mônica Cecília Bochetti Manna ◽  
Edna Frasson de Souza Montero ◽  
Maria Antonieta Longo Galvão da Silva ◽  
Yara Juliano

PURPOSE: To compare morphologically three different types of tracheotomy in growing rats, applying microsurgical technique. METHODS: EPM-1 Wistar growing rats (n=57) weighing 88gm and aged 35 days were randomized in four groups, according tracheotomy incision type (longitudinal, transverse and tracheal segment excision), and sham group. Following intramuscular anesthesia with ketamine and xylazine, the trachea was exposed and incised, according to the group, and a hand-made endotracheal cannula was inserted into the organ, under sterile conditions. This cannula was removed after 7 days, and animals have been sacrificed 30 days later. Tracheas samples were submitted to histological study, stained by hematoxylin-eosin and Masson trichrome, evaluating fibrosis, inflammatory infiltrate and epidermoid metaplasia. RESULTS: There was more frequency of inflammatory infiltrate at the tracheal epithelium in the tracheal segment excision group (87%) compared to the longitudinal (40%) and transverse (36%) incision groups (p=0.009). Evaluating epidermoid metaplasia, tracheal segment excision and the longitudinal groups presented 33% and 40%, respectively, compared to 0% of the transverse group (p=0.03). Concerning to fibrosis, in a global comparison (p=0.1) among the three groups there was no difference, however, compared to the longitudinal group the transverse group showed lower level of fibrosis (p=0.04). Sham group did not present any relevant morphologic alterations and it was used as reference pattern. CONCLUSION: Taken together, our data show that tracheal segment excision promotes more epithelium aggression and transverse tracheal incision shows less morphologic alterations.


2005 ◽  
Vol 2 (1) ◽  
pp. 81-84
Author(s):  
Kiyoyuki Yanaka ◽  
Muneyoshi Yasuda ◽  
Kentoaro Furusho ◽  
Hiroyuki Asakawa ◽  
Yuji Matsumaru ◽  
...  

Spontaneous intracerebral hematomas usually produce the sudden onset of devastating neurological symptoms. We describe a case showing slowly progressive clinical symptoms followed by a sudden deterioration caused by two different types of intracerebral hematomas coexisting in the adjacent area. A 72-year-old female with a 2-year history of Alzheimer’s disease and a 3-month history of occasional headache and vomiting was admitted after she experienced the sudden onset of right hemiparesis and a speech disturbance. Neuroradiological examinations demonstrated two different types of intracerebral hematomas coexisting in the left temporal lobe. The patient underwent a left frontotemporal craniotomy. A solid hematoma was found immediately below the cortex and a large hematoma cavity, which contained degraded bloody fluid, was found below the solid hematoma. Histological study demonstrated an arteriovenous malformation (AVM). The AVM may have been responsible for the pathogenesis of these two different types of intracerebral hematomas. Initially, a silent intracerebral hemorrhage from the AVM and liquefaction of the hematoma probably formed the cavity and repeated small subclinical hemorrhages into the cavity during a prolonged period of time may have caused the growth of the inner hematoma. The sudden rupture of the AVM most likely caused the outer solid hematoma, resulting in the abrupt onset of hemiparesis and speech disturbance. This is the first case to demonstrate two different types of intracerebral hematomas coexisting in the adjacent area. A possible mechanism for this rare condition is discussed. Nepal Journal of Neuroscience, Volume 2, Number 1, 2005


1990 ◽  
Vol 32 (2) ◽  
pp. 104-115 ◽  
Author(s):  
Tetsuo OKAMOTO ◽  
Mario Francisco Real GABRIELLI ◽  
Marisa Aparecida Cabrini GABRIELLI

2018 ◽  
Vol 26 (0) ◽  
Author(s):  
Osmar Aparecido Cuoghi ◽  
Lorraine Perciliano de Faria ◽  
Edilson Ervolino ◽  
Sônia Regina Panzarini Barioni ◽  
Francielle Topolski ◽  
...  

1963 ◽  
Vol s3-104 (67) ◽  
pp. 337-350
Author(s):  
D. E. JANS ◽  
K. F. A. ROSS

By double embedding whole isopods in paraffin wax and celloidin, after fixing them in Bodian's fluid, it was possible to obtain complete sets of undamaged serial sections through the thoracic and abdominal regions of females of Oniscus asellus L. and Porcellio scaber Latr., and also some incomplete series through males of these species and a species of the genus Armadillidium. These sections were stained by the methods of Bodian and of Holmes to show the nerve-fibres. They were then carefully studied, and the distribution and the morphology of all peripheral nerveendings and receptors in the body were determined as completely as possible. In all, 6 different types of terminations were found; and in some cases it was possible to draw valid inferences about their probable function from a consideration of their location and their morphology alone. Three of them are probably tactile. The function of the other three is much more uncertain; but one of them, a group of pit-like terminations situated on the under side of the lateral plates on each side of the thoracic segments, is particularly suitably located to function as a receptor of temperature or humidity, and morphologically it bears some resemblance to certain proven hygroreceptors in other arthropods. This particular termination and one of the other types, consisting of ventrally situated partially retracted hairs, do not appear to have been described previously in the land isopods.


Pharmaceutics ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1534
Author(s):  
Ekaterina Silina ◽  
Victor Stupin ◽  
Natalia Manturova ◽  
Vitaly Vasin ◽  
Konstantin Koreyba ◽  
...  

We studied the efficacy of using mesenchymal stem cells (MSC) and a polymeric compound (based on chitosan and cellulose with integrated cerium dioxide nanoparticles (PCCD)) in wound healing, and to compare the effects with various invasive and external drugs used for the same purpose. Two wounds were made on the backs of each of 112 Wistar rats, removing the skin. Eight groups were studied: Control_0—intact wounds; Control_ss—0.9% NaCl injections; MSC injections; Control_msc—intact wounds on the opposite side of the body from the MSC group; external application of the PCCD; external application of a combination of the drugs PCCD + MSC; DCh –ointment Dioxomethyltetrahydropyrimidine + Chloramphenicol; and DHCB—injections of a deproteinized hemoderivative of calf blood. After 14 days, we evaluated the state and size of the wounds, studied the level of microcirculation, performed a histological study, and identified and counted the different types of cells. The most effective remedy was combination PCCD + MSC. The treatments in the PCCD and MSC groups were more effective than in the DHCB and DCh groups. Invasive drugs and DCh slowed the regeneration process. DHCB did not affect the rate of healing for acute wounds without ischemia during the first week. The proven efficacy of developed polymeric compounds demonstrates the feasibility of further studies in clinical practice.


Author(s):  
Inese Kokina ◽  
Ēriks Sļedevskis ◽  
Vjačeslavs Gerbreders ◽  
Dace Grauda ◽  
Marija Jermaļonoka ◽  
...  

Frequency of callusogenesis, type and capacity of regeneration, cell ploidy, DNA methylation level, histological features, autofluorescence and photobleaching effects were studied in flax calli grown on medium supplemented with different concentrations of carbon (C) nanoparticles. The presence of carbon nanoparticles on medium decreased the percentage calli formation. Detailed histological study led to better understanding of changes in calli caused by nanoparticles. Ploidy variation in calli cells significantly depended on carbon nanoparticle concentration in the medium. Different types of calli regeneration associated with different C nanoparticles concentration in medium were observed: somatic embryogenesis (direct or indirect) or organogenesis. C nanoparticle dose-dependent DNA hypermethylation in flax calli cells were found. Increased repair ability during laser irradiation was found in calli grown on medium with carbon nanoparticles


2006 ◽  
Vol 309-311 ◽  
pp. 27-30 ◽  
Author(s):  
Faik N. Oktar ◽  
H. Sayrak ◽  
S. Ozsoy ◽  
Nurettin Heybeli

Different types of bone-graft substitutes have been developed and are in the market worldwide to eliminate the drawbacks of autogenous grafting. They vary in composition, strength, osteoinductive and osteoconductive properties, mechanism and rates by which they are resorbed and remodelled. Tooth derived hydroxyapatite (THA) is a novel biomaterial. This study was performed to determine the histological properties of THA on animals. A commercial coralline HA (CHA, Proosteon 200, Interpore Cross, USA) was used as control material. 20 sheeps were used and divided into 2 groups. Human THA (Group A) and CHA (Group B) materials were implanted to the tibiae of 10 sheeps for each group. The histological examinations of surrounding bone response were done 12 weeks after implantation. There was no significant difference histologically between group A and B. All materials were found to be surrounded by new bone tissue. THA was found to be as efficient as the standard CHA on histological basis. In addition, economical production of THA should be taken into consideration. Therefore, THA may be a viable alternative on bone grafting provided that clinical trials will be completed.


2020 ◽  
Vol 7 (2) ◽  
pp. 30-46
Author(s):  
I. A. Goroshinskaya ◽  
E. M. Frantsiyants ◽  
V. I. Aleynov ◽  
L. A. Nemashkalova ◽  
N. D. Cheryarina ◽  
...  

Purpose of the study. A comparative analysis of the intensity of lipid peroxidation and the functioning of various units of the antioxidant system in the blood of patients with malignant pancreatic lesions and with chronic pancreatitis.Patients and Methods. The content of malondialdehyde (MDA) and diene conjugates, the activity and content of antioxidant enzymes (superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase), the content of reduced glutathione and blood thiol status, as well as the content of vitamins E and A (as indicators of the non-enzymatic link of the antioxidant system), were studied in the blood of 51 patients before treatment and 22 donors. Based on a histological study of tumors, patients were divided into groups: neuroendocrine pancreatic cancer, pancreatic adenocarcinoma, pancreatic adenocarcinoma with a neuroendocrine component; a group of patients with chronic pancreatitis was also identified.Results. According to most of the studied parameters, neuroendocrine cancer differs from pancreatic adenocarcinoma in a more pronounced imbalance in the functioning of all units of the antioxidant system, which contributes to a greater intensification of free radical processes. Chronic pancreatitis was characterized by a lower content of all the studied antioxidant enzymes, as well as a decrease in the thiol status of the blood as compared with a malignant pancreatic lesion, which was possibly associated with the highest MDA level in the plasma of patients with chronic pancreatitis. At the same time, the content of vitamin E in chronic pancreatitis was significantly higher than for adenocarcinoma and adenocarcinoma with a neuroendocrine component.Conclusions. The most pronounced violation of oxidative status is one of the metabolic features of neuroendocrine cancer. Differences revealed in patients with neuroendocrine cancer, adenocarcinoma and pancreatitis can be useful both in the diagnosis of different types of pancreatic lesions and for assessing the state of patients during treatment.


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