scholarly journals Tracheotomy in growing rats: histological aspects

2003 ◽  
Vol 18 (3) ◽  
pp. 189-195 ◽  
Author(s):  
Mônica Cecília Bochetti Manna ◽  
Edna Frasson de Souza Montero ◽  
Maria Antonieta Longo Galvão da Silva ◽  
Yara Juliano

PURPOSE: To compare morphologically three different types of tracheotomy in growing rats, applying microsurgical technique. METHODS: EPM-1 Wistar growing rats (n=57) weighing 88gm and aged 35 days were randomized in four groups, according tracheotomy incision type (longitudinal, transverse and tracheal segment excision), and sham group. Following intramuscular anesthesia with ketamine and xylazine, the trachea was exposed and incised, according to the group, and a hand-made endotracheal cannula was inserted into the organ, under sterile conditions. This cannula was removed after 7 days, and animals have been sacrificed 30 days later. Tracheas samples were submitted to histological study, stained by hematoxylin-eosin and Masson trichrome, evaluating fibrosis, inflammatory infiltrate and epidermoid metaplasia. RESULTS: There was more frequency of inflammatory infiltrate at the tracheal epithelium in the tracheal segment excision group (87%) compared to the longitudinal (40%) and transverse (36%) incision groups (p=0.009). Evaluating epidermoid metaplasia, tracheal segment excision and the longitudinal groups presented 33% and 40%, respectively, compared to 0% of the transverse group (p=0.03). Concerning to fibrosis, in a global comparison (p=0.1) among the three groups there was no difference, however, compared to the longitudinal group the transverse group showed lower level of fibrosis (p=0.04). Sham group did not present any relevant morphologic alterations and it was used as reference pattern. CONCLUSION: Taken together, our data show that tracheal segment excision promotes more epithelium aggression and transverse tracheal incision shows less morphologic alterations.

2021 ◽  
Vol 10 (2) ◽  
pp. e21410212439
Author(s):  
Vanessa Sobue Franzo ◽  
Leiny Paula de Oliveira ◽  
Ana Carolina Pôssas Viana ◽  
Adriano Tony Ramos ◽  
Jéssica Fernanda Bertolino ◽  
...  

The Southern Caracara is a bird of prey, of the family Falconidae, inhabits all the Brazilian territory, they are omnivorous birds adapted for hunting. The digestive system of the predators presents differences in their morphology due to their eating habits. Knowing the importance of the gastrointestinal system, this study aims to carry out a histological study of the tongue, esophagus and the crop of Polyborus plancus. For this purpose, eight avian of both sexes, with different body weights and ages were used, they were euthanized and the organs were collected, fixed, dehydrated and stained with hematoxylin-eosin. It was noted that the tongue has stratified squamous epithelium with mucous glands and with different degrees of keratinization. The esophagus and crop have four well developed muscle layers and mucous glands. Additionally, in the crop there is presence of an adventitious layer that consists of loose connective tissue. We conclude that the tongue, the esophagus and the crop of the Southern Caracara have similar histological organization of several birds like the Pekin Ducks, ostriches, domestic chicken and partridges.


Author(s):  
Aku Heinonen ◽  
Heli Kivisaari ◽  
Radoslaw M. Michallik

AbstractThe occurrence of high-aluminum orthopyroxene megacrysts (HAOMs) in several massif-type Proterozoic anorthosite complexes has been used as evidence of their polybaric crystallization. Here, we report such petrographic and geochemical (XRF and EMPA) evidence from HAOMs discovered in the 1.64 Ga Ahvenisto rapakivi granite—massif-type anorthosite complex in southeastern Finland. Two different types of HAOMs were recognized: type 1 HAOMs are individual, euhedral-to-subhedral crystals, and up to 15 cm in diameter, and type 2 HAOMs occur in pegmatitic pockets closely associated with megacrystic (up to 30 cm long) plagioclase. The type 1 megacrysts in particular are surrounded by complex corona structures composed of plagioclase, low-Al orthopyroxene, iddingsite (after olivine), and sulfides. Orthopyroxene crystallization pressure estimates based on an Al-in-Opx geobarometer reveal a three-stage compositional evolution in both textural HAOM types. The Al content decreases significantly from the core regions of the HAOM (4.4–7.6 wt% Al2O3), through the rims (1.3–3.6 wt%), into the host rock (0.5–1.5 wt%). Enstatite compositions overlap, but are generally higher in the cores (En~60–70) and rims (En~50–70) of the HAOMs than in the host rock (En~45–60) orthopyroxenes. The highest recorded Al abundances in the HAOM cores correspond to crystallization pressures of up to ~ 1.1 GPa (~ 34 km depth), whereas the HAOM rims have crystallized at lower pressures (max. ~ 0.5 GPa, 20 km depth). The highest pressure estimates for the host rock orthopyroxene were ~ 0.2 GPa (< 7 km depth). These observations confirm the polybaric magmatic evolution of the Ahvenisto anorthosites and suggest that the entire 1.65–1.55 Ga Fennoscandian rapakivi suite was emplaced at a relatively shallow level (< 7 km depth) in the upper crust. Global comparison to similar rock types reveals remarkable similarities in the petrogenetic processes controlling HAOM composition and evolution of anorthosite parental magmas.


1959 ◽  
Vol 110 (5) ◽  
pp. 675-684 ◽  
Author(s):  
Bengt E. Gustafsson ◽  
Carl-Bertil Laurell

The earlier observed pronounced hypogammaglobulinemia in germfree rats of different ages has been confirmed. Using an immunologic technique the concentration of immunologic gamma globulins were found to vary between 10 and 15 per cent of the values observed in ordinary rats. Upon contamination of germfree rats with the normal microbial flora a pronounced lag phase was noted before the gamma globulin level became normal. This lag phase was most pronounced in growing rats. Newborn rats seem to start gamma globulin production more rapidly than older germfree rats. The response with regard to gamma globulin production on contamination of germfree rats with different types of bacterial cells through the natural routes is not identical.


1991 ◽  
Vol 105 (7) ◽  
pp. 556-557 ◽  
Author(s):  
J. A. J. Deans ◽  
J. Hill ◽  
M. Bennett

AbstractA histological study was performed of biopsies taken from jejunal free grafts used in pharyngeal reconstruction. The main findings were a decreased crypt/villi ratio and a mild chronic inflammatory infiltrate. There was no evidence of metaplastic or dysplastic transformation.


2012 ◽  
Vol 27 (1) ◽  
pp. 25-31 ◽  
Author(s):  
W. Wong-Ng ◽  
Q. Huang ◽  
I. Levin ◽  
J. C. Woicik ◽  
X. Shi ◽  
...  

The crystal structure of type I clathrate Ba8Ni4Ge42 has been determined using neutron powder diffraction, transmission electron microscopy (TEM, for possible superlattice), and extended X-ray absorption fine structure (EXAFS) measurements. Ba8Ni4Ge42 is cubic with the space group Pmn and unit-cell parameter a = 10.6769(2) Å (Dx = 5.988 g cm−3). The structure combines two different types of polyhedra: the dodecahedron (Ge20, 20-atom cage with 12 pentagonal faces) and the tetrakaidecahedron (Ge24, 24-atom cage with 12 pentagonal and 2 hexagonal faces). Each unit cell contains two Ge20 dodecahedra and six Ge24 tetrakaidecahedra. The Ge20 dodecahedra are linked via the interstitial 6c positions. The framework structure is formed by a tetrahedrally bounded network of Ge atoms, whereas Ba atoms reside inside the Ge20 and Ge24 cavities at the 2a and 6d crystallographic positions, respectively. Ni atoms exclusively occupy the 6c positions located on the hexagonal faces of the larger tetrakaidecahedra; no Ni atoms are found in the smaller dodecahedra that consist of pentagonal faces. A local structure study using EXAFS supports the coexistence of Ge and Ni on the 6c site. Electron diffraction in TEM reveals no detectable Ge/Ni ordering.


Author(s):  
Hamny Hamny ◽  
Muhammad Iqbal ◽  
Sri Wahyuni ◽  
Mustafa Sabri ◽  
M. Jalaluddin ◽  
...  

The aim of this research was to determine the anatomy and histology of pancreas of water monitor lizard (Varanus salvator). Two pancreas were used in this research (a male and a female). Water monitor lizard was euthanized using chloroform and subsequently necropsied to collect the pancreas. The pancreas was washed in 0.9 % NaCl solution. Anatomical observations were carried out including location, shape, and color of pancreas, also the length and weight measurements. Then, pancreas was fixed with 4% paraformaldehyde for 7 days to proceed to histological preparation and hematoxylin eosin (HE) staining. The results of the anatomical observations showed that pancreas of water monitor lizard have two lobes, dorsal lobe (splenic lobe) and ventral lobe (duodenal lobe). Dorsal lobe which was in oval form adhered with the spleen, and ventral lobe was located in the cranial of intestines with the form of long spherical. Both of pancreas lobes were white-yellow color with the length of 1.45±0.64 cm (dorsal lobe) and 7.00±0.42 cm (ventral lobe). Weight of dorsal lobe was 0.15±0.01 g and ventral lobe was 6.35±2.30 g. The histological observations found the acinar cells, ducts, and blood vessels in both of pancreas lobes. Additionally, the similar structure of Langerhans islet only found at the ventral lobe of pancreas. In conclusion, pancreas of water monitor lizard consists of two lobes which located differently. Ventral lobe is larger than the dorsal lobe. Both lobes have similar histological structure except the Langerhans islets are only appeared in the ventral lobe.


PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e107953 ◽  
Author(s):  
Yong-In Ju ◽  
Teruki Sone ◽  
Kazuhiro Ohnaru ◽  
Kensuke Tanaka ◽  
Hidetaka Yamaguchi ◽  
...  

2005 ◽  
Vol 2 (1) ◽  
pp. 81-84
Author(s):  
Kiyoyuki Yanaka ◽  
Muneyoshi Yasuda ◽  
Kentoaro Furusho ◽  
Hiroyuki Asakawa ◽  
Yuji Matsumaru ◽  
...  

Spontaneous intracerebral hematomas usually produce the sudden onset of devastating neurological symptoms. We describe a case showing slowly progressive clinical symptoms followed by a sudden deterioration caused by two different types of intracerebral hematomas coexisting in the adjacent area. A 72-year-old female with a 2-year history of Alzheimer’s disease and a 3-month history of occasional headache and vomiting was admitted after she experienced the sudden onset of right hemiparesis and a speech disturbance. Neuroradiological examinations demonstrated two different types of intracerebral hematomas coexisting in the left temporal lobe. The patient underwent a left frontotemporal craniotomy. A solid hematoma was found immediately below the cortex and a large hematoma cavity, which contained degraded bloody fluid, was found below the solid hematoma. Histological study demonstrated an arteriovenous malformation (AVM). The AVM may have been responsible for the pathogenesis of these two different types of intracerebral hematomas. Initially, a silent intracerebral hemorrhage from the AVM and liquefaction of the hematoma probably formed the cavity and repeated small subclinical hemorrhages into the cavity during a prolonged period of time may have caused the growth of the inner hematoma. The sudden rupture of the AVM most likely caused the outer solid hematoma, resulting in the abrupt onset of hemiparesis and speech disturbance. This is the first case to demonstrate two different types of intracerebral hematomas coexisting in the adjacent area. A possible mechanism for this rare condition is discussed. Nepal Journal of Neuroscience, Volume 2, Number 1, 2005


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