Inheritance and expression of stripe rust resistance in common wheat (Triticum aestivum) transferred from Aegilops tauschii and its utilization

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is a globally devastating disease of common wheat (Triticum aestivum L.), resulting in substantial economic losses. To identify effective resistance genes, a genome-wide association study was conducted on 120 common wheat lines from different wheat-growing regions of China using the wheat 90K iSelect SNP array. Seventeen loci were identified, explaining 9.5 to 21.8% of the phenotypic variation. Most of these genes were detected in the A (seven) and B (seven) genomes, with only three in the D genome. Among them, 11 loci were colocated with known resistance genes or quantitative trait loci reported previously, whereas the other six are likely new resistance loci. Annotation of flanking sequences of significantly associated SNPs indicated the presence of three important candidate genes, including E3 ubiquitin-protein ligase, F-box repeat protein, and disease resistance RPP13-like protein. This study increased our knowledge in understanding the genetic architecture for stripe rust resistance and identified wheat varieties with multiple resistance alleles, which are useful for improvement of stripe rust resistance in breeding.

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A new stripe rust resistance gene transferred from Thinopyrum intermedium to hexaploid wheat (Triticum aestivum)

Phytoprotection ◽

10.7202/044023ar ◽

2010 ◽

Vol 90 (2) ◽

pp. 57-63 ◽

Cited By ~ 13

Author(s):

Pei-Gao Luo ◽

Xue-Yun Hu ◽

Zhi-Jian Chang ◽

Min Zhang ◽

Huai-Qiong Zhang ◽

...

Keyword(s):

Triticum Aestivum ◽

Resistance Gene ◽

Common Wheat ◽

Stripe Rust ◽

Rust Resistance ◽

Stripe Rust Resistance ◽

Thinopyrum Intermedium ◽

Wheat Stripe Rust ◽

Intermediate Wheatgrass ◽

Cryptic Translocation

Wheat stripe rust (Puccinia striiforis f. sp. tritici) races CYR31 and CYR32, prevalent in China, are virulent to many wheat stripe rust resistance genes (Yr genes). To expand the availability of effective resistance to CYR31 and CYR32, stripe rust resistance was transferred from intermediate wheatgrass (Thinopyrum intermedium) to common wheat (Triticum aestivum). The susceptible wheat cultivar CM107 was crossed with amphiploid TAI7047, derived from the wide cross Taiyuan768/Thinopyrum intermedium//76(64). Two wheat lines originating from the cross, YU24 and YU25, were resistant to CYR31 and CYR32. Pedigree analysis showed that the resistance to stripe rust in YU24 and YU25 originated from intermediate wheatgrass. Genetic analyses indicated that the resistance to stripe rust is controlled by a single dominant gene. Allelic tests determined that the resistance gene(s) in YU24 and YU25 are identical. The new gene has temporarily been designated as YrYU25. SSR and RAPD analyses showed that YrYU25 was introduced by cryptic translocation into common wheat.

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Stripe rust resistance gene Yr34 (synonym Yr48) is located within a distal translocation of Triticum monococcum chromosome 5AmL into common wheat

Theoretical and Applied Genetics ◽

10.1007/s00122-021-03816-z ◽

2021 ◽

Author(s):

Shisheng Chen ◽

Joshua Hegarty ◽

Tao Shen ◽

Lei Hua ◽

Hongna Li ◽

...

Keyword(s):

Resistance Gene ◽

Common Wheat ◽

Stripe Rust ◽

Rust Resistance ◽

Triticum Monococcum ◽

Rust Resistance Gene ◽

Stripe Rust Resistance ◽

Polyploid Wheat ◽

Stripe Rust Resistance Gene ◽

A Genome

AbstractKey messageThe stripe rust resistance geneYr34 was transferred to polyploid wheat chromosome 5AL from T. monococcumand has been used for over two centuries.Wheat stripe (or yellow) rust, caused by Puccinia striiformis f. sp. tritici (Pst), is currently among the most damaging fungal diseases of wheat worldwide. In this study, we report that the stripe rust resistance gene Yr34 (synonym Yr48) is located within a distal segment of the cultivated Triticum monococcum subsp. monococcum chromosome 5AmL translocated to chromosome 5AL in polyploid wheat. The diploid wheat species Triticum monococcum (genome AmAm) is closely related to T. urartu (donor of the A genome to polyploid wheat) and has good levels of resistance against the stripe rust pathogen. When present in hexaploid wheat, the T. monococcum Yr34 resistance gene confers a moderate level of resistance against virulent Pst races present in California and the virulent Chinese race CYR34. In a survey of 1,442 common wheat genotypes, we identified 5AmL translocations of fourteen different lengths in 17.5% of the accessions, with higher frequencies in Europe than in other continents. The old European wheat variety “Mediterranean” was identified as a putative source of this translocation, suggesting that Yr34 has been used for over 200 years. Finally, we designed diagnostic CAPS and sequenced-based markers that will be useful to accelerate the deployment of Yr34 in wheat breeding programs to improve resistance to this devastating pathogen.

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Transfer of leaf rust and stripe rust resistance from Aegilops umbellulata Zhuk. to bread wheat (Triticum aestivum L.)

Genetic Resources and Crop Evolution ◽

10.1007/s10722-007-9289-3 ◽

2007 ◽

Vol 55 (6) ◽

pp. 849-859 ◽

Cited By ~ 27

Author(s):

Parveen Chhuneja ◽

Satinder Kaur ◽

R. K. Goel ◽

M. Aghaee-Sarbarzeh ◽

M. Prashar ◽

...

Keyword(s):

Triticum Aestivum ◽

Leaf Rust ◽

Bread Wheat ◽

Stripe Rust ◽

Rust Resistance ◽

Triticum Aestivum L ◽

Stripe Rust Resistance ◽

Aegilops Umbellulata

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Inheritance of temperature-sensitive leaf rust resistance and adult plant stripe rust resistance in common wheat cultivar PBW343

Euphytica ◽

10.1007/s10681-008-9841-z ◽

2008 ◽

Vol 166 (2) ◽

pp. 277-282 ◽

Cited By ~ 2

Author(s):

Dibendu Datta ◽

S. K. Nayar ◽

M. Prashar ◽

S. C. Bhardwaj

Keyword(s):

Leaf Rust ◽

Common Wheat ◽

Stripe Rust ◽

Rust Resistance ◽

Wheat Cultivar ◽

Leaf Rust Resistance ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Temperature Sensitive ◽

Common Wheat Cultivar

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Molecular mapping of YrTZ2, a stripe rust resistance gene in wild emmer accession TZ-2 and its comparative analyses with Aegilops tauschii

Journal of Integrative Agriculture ◽

10.1016/s2095-3119(17)61846-x ◽

2018 ◽

Vol 17 (6) ◽

pp. 1267-1275 ◽

Cited By ~ 4

Author(s):

Zhen-zhong WANG ◽

Jing-zhong XIE ◽

Li GUO ◽

De-yun ZHANG ◽

Gen-qiao LI ◽

...

Keyword(s):

Resistance Gene ◽

Stripe Rust ◽

Rust Resistance ◽

Molecular Mapping ◽

Aegilops Tauschii ◽

Wild Emmer ◽

Rust Resistance Gene ◽

Stripe Rust Resistance ◽

Stripe Rust Resistance Gene ◽

Emmer Accession

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Genetic Analysis and Molecular Mapping of an All-Stage Stripe Rust Resistance Gene in Triticum aestivum-Haynaldia villosa Translocation Line V3

Journal of Integrative Agriculture ◽

10.1016/s2095-3119(13)60293-2 ◽

2013 ◽

Vol 12 (12) ◽

pp. 2197-2208 ◽

Cited By ~ 6

Author(s):

Lu HOU ◽

Dong-fang MA ◽

Mao-lin HU ◽

Miao-miao HE ◽

Yan LU ◽

...

Keyword(s):

Triticum Aestivum ◽

Genetic Analysis ◽

Resistance Gene ◽

Stripe Rust ◽

Rust Resistance ◽

Molecular Mapping ◽

Stripe Rust Resistance ◽

Translocation Line ◽

Haynaldia Villosa ◽

Stripe Rust Resistance Gene

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Construction of a hexaploid wheat (Triticum aestivumL.) bacterial artificial chromosome library for cloning genes for stripe rust resistance

Genome ◽

10.1139/g05-078 ◽

2005 ◽

Vol 48 (6) ◽

pp. 1028-1036 ◽

Cited By ~ 13

Author(s):

P Ling ◽

X M Chen

Keyword(s):

Triticum Aestivum ◽

Stripe Rust ◽

Hexaploid Wheat ◽

Rust Resistance ◽

Puccinia Striiformis ◽

Bac Library ◽

Artificial Chromosome ◽

Stripe Rust Resistance ◽

Insert Size ◽

Bac Clones

A hexaploid wheat (Triticum aestivum L.) bacterial artificial chromosome (BAC) library was constructed for cloning Yr5 and other genes conferring resistance to stripe rust (Puccinia striiformis f. sp. tritici). Intact nuclei from a Yr5 near-isogenic line were used to isolate high molecular weight DNA, which was partially cleaved with HindIII and cloned into pECBAC1 and pIndigoBAC-5 vectors. The wheat BAC library consisted of 422 400 clones arrayed in 1100 micro-titer plates (each plate with 384 wells). Random sampling of 300 BAC clones indicated an average insert size of 140 kb, with a size range from 25 to 365 kb. Ninety percent of the clones in the library had an insert size greater than 100 kb and fewer than 5% of the clones did not contain inserts. Based on an estimated genome size of 15 966 Mb for hexaploid wheat, the BAC library was estimated to have a total coverage of 3.58× wheat genome equivalents, giving approximately 96% probability of identifying a clone representing any given wheat DNA sequence. Twelve BAC clones containing an Yr5 locus-specific marker (Yr5STS7/8) were successfully selected by PCR screening of 3-dimensional BAC pools. The results demonstrated that the T. aestivum BAC library is a valuable genomic resource for positional cloning of Yr5. The library also should be useful in cloning other genes for stripe rust resistance and other traits of interest in hexaploid wheat.Key words: BAC library, BAC pools, hexaploid wheat, Puccinia striiformis f. sp. tritici, resistance gene, stripe rust, Triticum aestivum.

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