QUANTITATIVE IMMUNOELECTROPHORETIC ANALYSIS OF SALMONELLA TYPHI ANTIGENS AND OF CORRESPONDING ANTIBODIES IN HUMAN SERA

2009 ◽  
Vol 88B (1-6) ◽  
pp. 237-242
Author(s):  
F. Espersen ◽  
J. B. Hertz ◽  
N. HøIby ◽  
H. H. Mogensen
Keyword(s):  
Salmonella Typhi ◽  
Immunoelectrophoretic Analysis ◽  
Human Sera

A RAPID SLIDE TEST FOR THE DETECTION OF VI ANTIBODY IN HUMAN AND ANIMAL SERA

1964 ◽  
Vol 10 (4) ◽  
pp. 551-554
Author(s):  
R. Wallace ◽  
B. B. Diena ◽  
L. Greenberg
Keyword(s):  
Growth Medium ◽  
Intact Cell ◽  
Salmonella Typhi ◽  
Test Tube ◽  
Cell Agglutination ◽  
Flocculation Test ◽  
Slide Test ◽  
Human Sera ◽  
Slide Technique ◽  
Vi Antigen

A rapid slide test for the detection of Vi antibodies, using spheroplasts of Salmonella typhi (Vi I, Bhatnagar strain) as the test antigen, has been described. The spheroplasts, grown in a semisynthetic medium containing 1.5% glycine, maintained their antigenic sensitivity for periods of at least 1 month when stored in the cold in the growth medium preserved with 1:5000 Thimerosal.Comparative tests were carried out with the spheroplast slide technique, agglutination tests using intact Bhatnagar cells and Vi-antigen-sensitized red cells, and flocculation tests of Vi-antigen-sensitized bentonite particles. The slide test proved to be specific and reproducible and was as sensitive as the test tube hemagglutination test for both rabbit and human sera. The intact cell agglutination technique and the bentonite flocculation test were relatively insensitive.

scholarly journals The anti-globulin technique applied to the detection of non-agglutinating antibody against salmonella typhi O in human sera

1950 ◽  
Vol 48 (3) ◽  
pp. 357-360 ◽  
Author(s):  
F. S. Stewart ◽  
J. D. McKeever
Keyword(s):  
Salmonella Typhi ◽  
Normal Individuals ◽  
Human Sera ◽  
History Of ◽  
Agglutinin Titre

A technique for carrying out the anti-globulin test with Salm. typhi O suspension in described; this technique has been used to examine a number of human sera. The degree of amplification of agglutinin titre and the final titres obtained were appreciably greater in the sera of individuals with a history of Salm. typhi infection, some of whom were carriers, than in the sera of normal individuals.

Immunoelectrophoretic Analysis of Membrane Glycoproteins in Cultured Human Endothelial Cells

1990 ◽  
Vol 63 (02) ◽  
pp. 303-311
Author(s):  
Tone Børsum
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Rabbit Antiserum ◽  
Platelet Glycoprotein ◽  
Membrane Glycoproteins ◽  
Human Endothelial Cells ◽  
Immunoelectrophoretic Analysis ◽  
Glycoprotein Complex ◽  
Crossed Immunoelectrophoresis ◽  
Triton X

SummaryHuman endothelial cells isolated from umbilical cordswere solubilized in Triton X-100 and examined by crossedimmunoelec-trophoresis using rabbit antiserum against endothelial cells. Endogenous labelling of the endothelialcell proteins with 14Cmannose followed by crossed immunoelectrophoresis and autoradiography revealed about 10 immunoprecipitates. Four of these endothelial cell glycoproteins were labelled by lactoperoxidase catalyzed iodination and thus were surface located. Three of the surface located glycoproteins showed reduced electrophoretic mobility after incubation of the endothelial cells with neuraminidase and were therefore sialoglycoproteins. Amphiphilicity of endothelial cell glycoproteins was studied by crossed hydrophobic interaction immunoelectrophoresis with phenyl-Sepharose in the intermediate gel. Amphiphilic proteins also show increasing electrophoretic migration velocity with decreasing concentration of Triton X-100 in the first dimension gels. Five of the endothelial cell glycoproteins were shown to be amphiphilic using these two techniques.Two monoclonal antibodies against the platelet glycoprotein complex Ilb-IIIa and glycoprotein IlIa, respectively, reacted with the same precipitate of endothelial cells. When a polyclonal antibody against the platelet glycoprotein complex Ilb-IIIa was incorporated into the intermediate gel the position of two endothelial cell precipitates were lowered. One of these was a sialoglycoprotein.

Acquired Disorder of Platelet Function Associated with Autoantibodies against Membrane Glycoprotein IIb-IIIa Complex - 1. Glycoprotein Analysis

1991 ◽  
Vol 65 (05) ◽  
pp. 491-496 ◽  
Author(s):  
M Meyer ◽  
C M Kirchmaier ◽  
A Schirmer ◽  
P Spangenberg ◽  
Ch Ströhl ◽  
...  
Keyword(s):  
Platelet Count ◽  
Platelet Aggregation ◽  
Platelet Adhesion ◽  
Bleeding Time ◽  
Membrane Glycoprotein ◽  
Membrane Glycoproteins ◽  
Abnormal Behaviour ◽  
Thrombocytopenic Purpura ◽  
Immunoelectrophoretic Analysis ◽  
Prolonged Bleeding Time

SummaryA patient with idiopathic thrombocytopenic purpura developed after splenectomy a thrombasthenia-like severe haemor-rhagic diathesis characterized by a normal or subnormal platelet count, prolonged bleeding time, strongly reduced platelet adhesion to glass and defective platelet aggregation in response to ADP and collagen. In contrast to hereditary thrombasthenia membrane glycoproteins (GP) lib and Ilia were normally present in the patient’s platelets. Immunoelectrophoretic analysis revealed an abnormal behaviour of the patient’s GP IIb-IIIa complex. Autoantibodies against GP IIb-IIIa were detected in Triton-extracted washed platelets. Incubation of normal platelets with plasma from the patient resulted in a similar immunoelectrophoretic abnormality of the GP IIb-IIIa complex indicating that bound autoantibodies (IgG) are responsible for the abnormal immunoelectrophoretic behaviour of the patient’s GP IIb-IIIa complex. Platelet fibrinogen was severely reduced similar to classical thrombasthenia suggesting that the GP IIb-IIIa complex is involved in platelet fibrinogen storage.

Detection of dengue virus in Aedes mosquitoes in Delhi, India

ENTOMON ◽  
2019 ◽  
Vol 44 (3) ◽  
pp. 213-218
Author(s):  
Suresh Chand Kaushik ◽  
Sukhvir Singh ◽  
Purnima Srivastava ◽  
R. Rajendran
Keyword(s):  
Early Intervention ◽  
Early Detection ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Infection Rate ◽  
Transmission Season ◽  
Aedes Mosquitoes ◽  
Alternative Approach ◽  
Human Sera ◽  
Minimum Infection Rate

Detection of viruses in human sera particularly in endemic areas is cumbersome and laborious. Therefore, an alternative approach, Immuno-fluorescence assay (IFA) was performed to determine dengue virus (DENV) positivity in mosquitoes. A total of 1055 adult Aedes aegypti female mosquitoes were tested for IFA test against DENV. Minimum infection rate (MIR) for DENV was found higher during August to November 2016 ranging from 10.75 to 20.83. The average yearly MIR was about 6.64. Higher MIR for Ae. aegypti was found in Sarfabad, Noida (12.71) and Khoda Colony, Ghaziabad (11.90). Minimum MIR (4.67) was observed in Sanjay colony (Faridabad). The main contribution of this study resides in the development of a more suitable monitoring system for early detection of viral circulation and to prioritize early intervention in the non-transmission season.

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