THIN LAYER ISO-ELECTRO FOCUSING OF INTRACELLULAR BACTERIAL PROTEIN IN CLASSIFICATION OF SOME REPRESENTATIVES OF THE FAMILIES ENTEROBACTERIACEAE AND VIBRIONACEAE

2009 ◽  
Vol 90B (1-6) ◽  
pp. 383-388
Author(s):  
JAN GJERDE
Keyword(s):  
Thin Layer ◽  
Bacterial Protein

scholarly journals Self-Organising Maps for the Exploration and Classification of Thin-Layer Chromatograms

Talanta ◽  
2021 ◽  
pp. 122460
Author(s):  
Matthias Guggenberger ◽  
Josua T. Oberlerchner ◽  
Heinrich Grausgruber ◽  
Thomas Rosenau ◽  
Stefan Böhmdorfer
Keyword(s):  
Thin Layer

The use of thin-layer chromatography for the separation and identification of phytoplankton pigments

1965 ◽  
Vol 45 (3) ◽  
pp. 583-591 ◽  
Author(s):  
J. P. Riley ◽  
T. R. S. Wilson
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Phaeodactylum Tricornutum ◽  
Isochrysis Galbana ◽  
Marine Phytoplankton ◽  
Rapid Separation ◽  
Systematic Classification ◽  
Layer Chromatography ◽  
Natural Phytoplankton

Thin-layer chromatography has been used for the rapid separation of microgram amounts of pigments of marine phytoplankton, both from cultures and from the sea. The separation is carried out on plates coated with silica gel; the chromatogram is developed with a solvent consisting of petroleum ether (b.p. 60–80°), ethyl acetate and diethylamine in the ratio 58:30:12 by volume. The separated individual pigments may be eluted from the plate and characterized by their absorption spectra. The pigments of the following phytoplankton have been examined: Dunaliella primolecta, Phaeodactylum tricornutum, Isochrysis galbana, Dicrateria inornata, Coccolithus huxleyi, and Peridinium trochoidium.IntroductionA knowledge of the component pigments of phytoplankton is of value in the systematic classification of these organisms and in the study of the mechanism of photosynthesis. Very little is known about the amounts of the lesser phyto-plankton pigments associated with the natural phytoplankton crop in the sea, but a considerable amount of work has been carried out on the measurement of chlorophylls a, b and c and of carotenoids for the estimation of the plant bio-mass in the sea. These determinations are usually carried out by modifications of the poly-chromatic spectrophotometric method originally described by Richards with Thompson (1952) and modified by Creitz & Richards (1955) (see also Parsons & Strickland, 1963). While these procedures give a satisfactory measure of chlorophylls a and b, their precision for chlorophyll c and carotenoids is poor; furthermore, no resolution of carotenes from xanthophylls can be attained; nor can any indication of the presence of unusual pigments be obtained.

scholarly journals Predictive assessment of the fluid loss properties of thin-layer reservoirs of Vikulovskaya series based on the results of core and well logs

Georesursy ◽  
2021 ◽  
Vol 23 (2) ◽  
pp. 170-178
Author(s):  
Tatyana G. Isakova ◽  
Tatyana F. Diakonova ◽  
Alena D. Nosikova ◽  
Georgy A. Kalmykov ◽  
Alexander V. Akinshin ◽  
...  
Keyword(s):  
Thin Layer ◽  
Well Logs ◽  
Fluid Loss ◽  
Pore Sizes ◽  
The Core ◽  
Total Filtration ◽  
Predictive Assessment ◽  
Filtration Capacity

The aim of the work is to predict the filtration capacity of reservoirs based on core and well logs data at the stage of petrophysical study of rocks before the start of active development of the object. All the results were obtained from the data of porometric characteristics of rocks on the example of the vikulovskaya series’s deposits of the Krasnoleninsky arch. The patterns of changes in pore sizes and their contribution to the total filtration depending on the lithophysical type of the rock were established on the core plug. A classification of rocks by pore radii is proposed, and a method for assessing the filtration capacity of reservoirs based on well logs data is developed, with the calculation of the share of each layer in the planned perforation interval

scholarly journals DEMONSTRATION OF TYPE SPECIFIC PROTEINS IN EXTRACTS OF FUSOBACTERIA

1938 ◽  
Vol 67 (1) ◽  
pp. 49-59 ◽  
Author(s):  
Charles Weiss ◽  
Dora G. Mercado
Keyword(s):  
Additional Data ◽  
Proteolytic Enzymes ◽  
Bacterial Protein ◽  
Experimental Animals ◽  
Biochemical Characteristics ◽  
Mass Cultures ◽  
Group A ◽  
Specific Proteins ◽  
Hydrolysis Of

1. The classification of fusobacteria into types, as suggested by Slanetz and Rettger on the basis of their cultural and biochemical characteristics, was confirmed. The following additional data are presented: the behavior of types I, II, and III in several previously untested carbohydrates; the final pH in 1 per cent glucose broth; the hydrolysis of starch; the failure to decompose sodium hippurate and citrates; the absence of pathogenicity for several types of experimental animals; and the absence of fibrinolysin and of proteolytic enzymes. 2. By the use of a technic described in the text, it was possible to grow the anaerobic fusobacteria in mass cultures. 3. With the methods of Heidelberger and Kendall, immunologically type specific proteins were extracted from fusobacteria types I, II, and III. This observation, which is in a sense an extension of the work of Lancefield on Streptococcus hemolyticus, group A, represents the second instance of a bacterial protein functioning as a determinant of bacterial types. 4. Preliminary data indicate that group specificity depends upon a carbohydrate which is extractable by the methods of Heidelberger and Avery.

Thin-layer chromatographic classification of antibiotics exhibiting antitumor properties

1977 ◽  
Vol 133 (2) ◽  
pp. 291-301 ◽  
Author(s):  
H.J. Issaq ◽  
E.W. Barr ◽  
T. Wei ◽  
C. Meyers ◽  
A. Aszalos
Keyword(s):  
Thin Layer ◽  
Antitumor Properties

TRICHOPHYTONS: CHEMICAL CLASSIFICATION AND THE ISOLATION OF TONSOPURPURIN AND STEROID E

1965 ◽  
Vol 11 (5) ◽  
pp. 869-876 ◽  
Author(s):  
A. Ho van Hap ◽  
J. A. Omelon ◽  
G. R. Duncan ◽  
G. C. Walker
Keyword(s):  
Thin Layer ◽  
Petroleum Ether ◽  
Silica Gel ◽  
Methylene Chloride ◽  
Physical Characteristics ◽  
Chemical Classification

Chromatography of the methylene chloride extracts of the dried mycelium of a number of Trichophyton species on a thin layer of silica gel may supplement other methods of classification of Trichophyton species. An unidentified steroid has been isolated from petroleum ether extracts of T. tonsurans 327, as well as brassicasterol and tonsopurpurin. The latter appears to be a pigment which has the chemical and physical characteristics of a naphthoquinone.

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