scholarly journals Seasonal variation in semen quality of swamp buffalo bulls (Bubalus bubalis) in Thailand

2007 ◽  
Vol 9 (1) ◽  
pp. 92-101 ◽  
Author(s):  
Seri Koonjaenak ◽  
Vichai Chanatinart ◽  
Suneerat Aiumlamai ◽  
Tanu Pinyopumimintr ◽  
Heriberto Rodriguez-Martinez
2016 ◽  
Vol 3 (3) ◽  
pp. 97
Author(s):  
Muhammad Riyadhi ◽  
Akbar Budiansa ◽  
Muhammad Rizal

The purpose of this research was evaluate the quality of spermatozoa concentration in the caput, corpus and cauda of the swamp buffalo epididymis (Bubalus bubalis carabanensis).  Method of this research was to exploration to 13 epididymides of eight swamp buffaloes were obtained from Banjar and Banjarmasin slaughterhouses,evaluated the quality of spermatozoa in caput, corpus and cauda of epididymis.  Quality of collected-spermatozoa including spermatozoa motility, percentage of live spermatozoa, spermatozoa concentration and percentage of abnormality.  Result of this study showed that mean of each of caput spermatozoa motility, percentage of live spermatozoa, spermatozoa concentration and percentage of abnormality; 0%, 45.43% (31.87–72%), 189,62 x106 (40–480 x106) and 56.16 %(44.34–66.53%), corpus ;2.77% (1–9%), 58.73% (45.14 –76%), 152.31 x106 (45 – 345x106), and 47.61 %(23.92 – 60.15%), cauda;53.46% (20 – 70%), 74.32 % (56.68 – 83%), 1,459.62 x106 (825 – 2,340x106), and 34.60%(15.89 –50.04%). In conclusion, spermatozoaofcaudaepididymis could be used in artificial insemination program.Keywords: Spermatozoa, epididymis, swamp buffalo.


1942 ◽  
Vol 25 (9) ◽  
pp. 815-826 ◽  
Author(s):  
R.E. Erb ◽  
F.N. Andrews ◽  
J.H. Hilton

2022 ◽  
Vol 335 ◽  
pp. 00044
Author(s):  
Ardyah Ramadhina Irsanti Putri ◽  
Gatot Ciptadi ◽  
Agus Budiarto ◽  
Sri Rahayu ◽  
Helly Nurul Karima ◽  
...  

Chromosomal data on swamp buffalo especially in East Java province, is still lacking. For breeders, chromosomal analysis needs to be done to detect the possibility of hereditary genetic defects. In Malang Area, farmers lack of care and breeding programs for swamp buffalo also caused a decline in the genetic quality and performance. The purpose of this study was to compare the results of the chromosome analysis of swamp buffalo in Malang and another area in East Java province, which were selected based on differences in phenotypic characters. Chromosome analysis is very important for breeders because the results of this study can be used as a consideration for strategies to improve the genetic quality of swamp buffalo. The method used is standard karyotyping using whole blood with G banding staining. Chromosomal preparation using Karyo MAX medium, Colcemic Solution, Giemsa Stain, and KCl solution. Cell culture was performed according to the standard karyotyping method in mammals. The minimum number of 5 spreading chromosomes is the best was chosen, microphotographed, and then chromosome analysis is performed using Cytovision Image Analysis software. The results of this study did not find swamp buffalo with an abnormal number of chromosomes in East Java Province, Indonesia.


1944 ◽  
Vol 27 (4) ◽  
pp. 303-310 ◽  
Author(s):  
Eric W. Swanson ◽  
H.A. Herman

Author(s):  
Kartini Eriani ◽  
Nisa Sari ◽  
Rosnizar Rosnizar ◽  
Dasrul Dasrul ◽  
Suhartono Suhartono ◽  
...  

<p class="IsiAbstrakIndo">Aceh swamp buffaloes<span lang="AR-YE" dir="RTL"> </span><em>(Bubalus bubalis) </em>are decreasing their population and genetic quality. This study was conducted to determine the influence of lactose and glycerol cryoprotectants on spermatozoa of Aceh swamp buffaloes<span lang="AR-YE" dir="RTL"> </span><span lang="EN-GB">after</span><span lang="AR-YE" dir="RTL"> </span>thawing<em>.</em><span lang="AR-YE" dir="RTL"> </span><span lang="EN-GB">This study used completely</span>a factorial randomized design with nine treatments, and five replications. Fresh semen of the Aceh swamp buffalo were diluted by using a combination extender lactose cryoprotectants 0 mM (L<sub>0</sub>), 60 mM (L<sub>60</sub>), 120 mM (L<sub>120</sub>) and glycerol 3% (G<sub>3</sub>), 5% (G<sub>5</sub>), 7% (G<sub>7</sub>) with the equilibration of 4 hours. The results showed that the combination of cryoprotectants L<sub>120</sub>G<sub>7 </sub>influenced significantly (P &lt; 0.05) on the quality of spermatozoa of the Aceh swamp buffalo <em>(B. bubalis)</em><span lang="AR-YE" dir="RTL"> </span><span lang="EN-GB">after</span><span lang="AR-YE" dir="RTL">  </span>thawing<em>.</em><span lang="AR-YE" dir="RTL"> </span><span lang="EN-GB">The percentage of sperm motility L</span><sub>120</sub>G<sub>7</sub> (42.60 ± 1.14); viability L<sub>120</sub>G<sub>7 </sub>(55.00 ± 0.71);  acrosome integrity L<sub>120</sub>G<sub>7</sub> (52.00 ± 0.71); and plasma membrane integrity L<sub>120</sub>G<sub>7</sub><span lang="AR-YE" dir="RTL"> (</span>53.20 ± 1.48).  The combination of lactose cryoprotectants 120 mM (L<sub>120</sub>) and glycerol 7% (G<sub>7</sub>) was the best combination to maintain the quality of spermatozoa of swamp buffalo. This finding could be used to define a policy for the spermatozoa storage of Aceh swamp buffalo to artificial insemination (AI). </p>


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