scholarly journals A review chromosome analysis comparison of swamp buffalo (Bubalus bubalis) with different phenotypic characters in East Java, Indonesia

2022 ◽  
Vol 335 ◽  
pp. 00044
Author(s):  
Ardyah Ramadhina Irsanti Putri ◽  
Gatot Ciptadi ◽  
Agus Budiarto ◽  
Sri Rahayu ◽  
Helly Nurul Karima ◽  
...  

Chromosomal data on swamp buffalo especially in East Java province, is still lacking. For breeders, chromosomal analysis needs to be done to detect the possibility of hereditary genetic defects. In Malang Area, farmers lack of care and breeding programs for swamp buffalo also caused a decline in the genetic quality and performance. The purpose of this study was to compare the results of the chromosome analysis of swamp buffalo in Malang and another area in East Java province, which were selected based on differences in phenotypic characters. Chromosome analysis is very important for breeders because the results of this study can be used as a consideration for strategies to improve the genetic quality of swamp buffalo. The method used is standard karyotyping using whole blood with G banding staining. Chromosomal preparation using Karyo MAX medium, Colcemic Solution, Giemsa Stain, and KCl solution. Cell culture was performed according to the standard karyotyping method in mammals. The minimum number of 5 spreading chromosomes is the best was chosen, microphotographed, and then chromosome analysis is performed using Cytovision Image Analysis software. The results of this study did not find swamp buffalo with an abnormal number of chromosomes in East Java Province, Indonesia.

Author(s):  
Kartini Eriani ◽  
Nisa Sari ◽  
Rosnizar Rosnizar ◽  
Dasrul Dasrul ◽  
Suhartono Suhartono ◽  
...  

<p class="IsiAbstrakIndo">Aceh swamp buffaloes<span lang="AR-YE" dir="RTL"> </span><em>(Bubalus bubalis) </em>are decreasing their population and genetic quality. This study was conducted to determine the influence of lactose and glycerol cryoprotectants on spermatozoa of Aceh swamp buffaloes<span lang="AR-YE" dir="RTL"> </span><span lang="EN-GB">after</span><span lang="AR-YE" dir="RTL"> </span>thawing<em>.</em><span lang="AR-YE" dir="RTL"> </span><span lang="EN-GB">This study used completely</span>a factorial randomized design with nine treatments, and five replications. Fresh semen of the Aceh swamp buffalo were diluted by using a combination extender lactose cryoprotectants 0 mM (L<sub>0</sub>), 60 mM (L<sub>60</sub>), 120 mM (L<sub>120</sub>) and glycerol 3% (G<sub>3</sub>), 5% (G<sub>5</sub>), 7% (G<sub>7</sub>) with the equilibration of 4 hours. The results showed that the combination of cryoprotectants L<sub>120</sub>G<sub>7 </sub>influenced significantly (P &lt; 0.05) on the quality of spermatozoa of the Aceh swamp buffalo <em>(B. bubalis)</em><span lang="AR-YE" dir="RTL"> </span><span lang="EN-GB">after</span><span lang="AR-YE" dir="RTL">  </span>thawing<em>.</em><span lang="AR-YE" dir="RTL"> </span><span lang="EN-GB">The percentage of sperm motility L</span><sub>120</sub>G<sub>7</sub> (42.60 ± 1.14); viability L<sub>120</sub>G<sub>7 </sub>(55.00 ± 0.71);  acrosome integrity L<sub>120</sub>G<sub>7</sub> (52.00 ± 0.71); and plasma membrane integrity L<sub>120</sub>G<sub>7</sub><span lang="AR-YE" dir="RTL"> (</span>53.20 ± 1.48).  The combination of lactose cryoprotectants 120 mM (L<sub>120</sub>) and glycerol 7% (G<sub>7</sub>) was the best combination to maintain the quality of spermatozoa of swamp buffalo. This finding could be used to define a policy for the spermatozoa storage of Aceh swamp buffalo to artificial insemination (AI). </p>


2016 ◽  
Vol 3 (3) ◽  
pp. 97
Author(s):  
Muhammad Riyadhi ◽  
Akbar Budiansa ◽  
Muhammad Rizal

The purpose of this research was evaluate the quality of spermatozoa concentration in the caput, corpus and cauda of the swamp buffalo epididymis (Bubalus bubalis carabanensis).  Method of this research was to exploration to 13 epididymides of eight swamp buffaloes were obtained from Banjar and Banjarmasin slaughterhouses,evaluated the quality of spermatozoa in caput, corpus and cauda of epididymis.  Quality of collected-spermatozoa including spermatozoa motility, percentage of live spermatozoa, spermatozoa concentration and percentage of abnormality.  Result of this study showed that mean of each of caput spermatozoa motility, percentage of live spermatozoa, spermatozoa concentration and percentage of abnormality; 0%, 45.43% (31.87–72%), 189,62 x106 (40–480 x106) and 56.16 %(44.34–66.53%), corpus ;2.77% (1–9%), 58.73% (45.14 –76%), 152.31 x106 (45 – 345x106), and 47.61 %(23.92 – 60.15%), cauda;53.46% (20 – 70%), 74.32 % (56.68 – 83%), 1,459.62 x106 (825 – 2,340x106), and 34.60%(15.89 –50.04%). In conclusion, spermatozoaofcaudaepididymis could be used in artificial insemination program.Keywords: Spermatozoa, epididymis, swamp buffalo.


2020 ◽  
Vol 8 (2) ◽  
pp. 80-85
Author(s):  
D. A. P. Sari ◽  
Muladno ◽  
S. Said

Reproductive performance of female cattle is one of the important factors that need to be known in supporting the efficiency of beef cattle breeding programs so that the dam can produce calves every year. This study aimed to evaluate the potential and reproductive performance of female Bali cattle to preparing a baseline for cattle breeding production in SL-SPR Maju Bersama, Sungai Lilin District. Three hundred and thirty female Bali cattle owned by 247 farmers were measured and recorded based on their production and reproduction performance. Data were collected by interview and observation. Data were analyzed using statistical and descriptive methods. Reproductive performance obtained is the age first calving 1098±120 days; calving interval 457,25±8,75 days; days open 109,25±8,57 days and service per conception 5,46±0,42. The average female cattle have a BCS value of 3 (43%). The result showed that the breeding of Bali cattle at SL-SPR had sufficient potential, but it was necessary to improve performance. The basic data obtained can be used to start a breeding business to increase populations and improve the genetic quality of Bali cattle.


2007 ◽  
Vol 9 (1) ◽  
pp. 92-101 ◽  
Author(s):  
Seri Koonjaenak ◽  
Vichai Chanatinart ◽  
Suneerat Aiumlamai ◽  
Tanu Pinyopumimintr ◽  
Heriberto Rodriguez-Martinez

Genome ◽  
1989 ◽  
Vol 32 (6) ◽  
pp. 999-1002 ◽  
Author(s):  
M. Harisah ◽  
T. I. Azmi ◽  
M. Hilmi ◽  
M. K. Vidyadaran ◽  
T. A. Bongso ◽  
...  

Chromosome analysis on different breed types of water buffaloes (Bubalus bubalis) was undertaken to identify their karyotypes and to determine the pattern of chromosome segregation in crossbred water buffaloes. Altogether, 75 purebred and 198 crossbred buffaloes including 118 from Malaysia and 80 from the Philippines, were analyzed in this study. The diploid chromosome number of the swamp buffalo from both countries was 48 and.that of the river buffalo was 50, while all F1 hybrids exhibited 49 chromosomes. The F2 hybrids consisted of three different karyotype categories (2n = 48, 2n = 49, and 2n = 50), whereas the backcrosses included two different karyotype categories each, with 2n = 48 and 2n = 49 in the three quarters swamp types and 2n = 49 and 2n = 50 in the three quarters river types. Chi-square tests on pooled data from Malaysia and the Philippines indicated that the distribution of different karyotype categories of F2 animals did not deviate significantly from the 1:2:1 ratio expected if only balanced gametes with 24 and 25 chromosomes were produced by the F1 hybrids. In the three quarters swamp and three quarters river types, the respective karyotypic categories were in ratios approximating 1:1. The distribution of chromosome categories among the F2 hybrids and backcrosses suggests that only genetically balanced gametes of the F1 hybrids are capable of producing viable F2 and backcross generations.Key words: buffaloes, chromosome segregation, genotype, buffalo hybrids.


2014 ◽  
Vol 8 (1) ◽  
Author(s):  
Gian Dina Febriani ◽  
Hamdan Hamdan ◽  
Juli Melia

This research aims to know the influence of the equilibration to the quality of the swamp buffalo (Bubalus bubalis) cement after thawing including: motility, and percentage of live spermatozoa. This research used the fresh cement from a 4 years old water buffalo was collected using an artificial vagina,and evaluation cement for macroscopic and microskopic to see if cement was worthy or not worthy to frozen. Good quality cement was diluted by adding Andromed, then semen were incorporated into the straw using the machine filling sealing followed by equilibrated with 3 treatment groups. Group I (n = 3 straw) with equilibration time of 3 hours, the Group II (n = 3 straw) with a time of equilibration was 5 hours, and group III (n = 3 straw) with equilibration a time of 7 hours. After equilibrated, straw frozen and stored in liquid N2 (BIBD Tuah Sakato procedures). The results show that the length of time the real equilibration effect (P 0.05) on the motility and percentage life of swamp buffalo spermatozoa. Equilibration 5 hours of time differs markedly with equilibration time 3 and 7 hours, motility, and percentage of live spermatozoa (44.15±1.70; 46.50±1.36 vs 14,55±1,58; 1.42±37.38 vs. 15.37±1.58; and 37.50±1.25). In conclusion, equilibration time is showed on the cement of swmp buffalo after 5 hours thawing.Key words: equilibration, swamp buffalo, thawing


2019 ◽  
Vol 1 (1) ◽  
pp. 92
Author(s):  
Fazidah Hanim Husain

Lighting is one of the key elements in any space and building infrastructure. Good design for an area in the building requires sufficient light that contributes to the efficiency of the activities. The correct method allows natural light to transmit, reduce heat and glare in providing a conducive learning environment. Light plays a significant influence to the quality of space and contributes focus of the students in an architecture studio. Previous research has shown that the effect of light also controlled emotions, behavior, and mood of the students. The operations of artificial lighting that have been used most of the time in an architecture studio during day and night may create lavishness and inadequacy at the same time. Therefore, this paper focuses on the identifying the quality of light for the architecture studio in UiTM (Perak), to instill a creative learning environment. Several methodologies adopted in this study such as illuminance level measurement using lux meter (LM-8100), and a questionnaire survey in gauging the lighting comfort level from students’ perspective. The study revealed that the illuminance level in the architecture studio is insufficient and not in the acceptable range stated in the Malaysian: Standards 1525:2007 and  not evenly distributed.  The study also concluded that the current studio environment is not condusive and appears monotonous. 


2020 ◽  
Vol 16 (4) ◽  
pp. 730-744
Author(s):  
V.I. Loktionov

Subject. The article reviews the way strategic threats to energy security influence the quality of people's life. Objectives. The study unfolds the theory of analyzing strategic threats to energy security by covering the matter of quality of people's life. Methods. To analyze the way strategic threats to energy security spread across cross-sectoral commodity and production chains and influences quality of people's living, I applied the factor analysis and general scientific methods of analysis and synthesis. Results. I suggest interpreting strategic threats to energy security as risks of people's quality of life due to a reduction in the volume of energy supply. I identified mechanisms reflecting how the fuel and energy complex and its development influence the quality of people's life. The article sets out the method to assess such quality-of-life risks arising from strategic threats to energy security. Conclusions and Relevance. In the current geopolitical situation, strategic threats to energy security cause long-standing adverse consequences for the quality of people's life. If strategic threats to energy security are further construed as risk of quality of people's life, this will facilitate the preparation and performance of a more effective governmental policy on energy, which will subsequently raise the economic well-being of people.


2015 ◽  
Vol 6 (1) ◽  
pp. 50-57
Author(s):  
Rizqa Raaiqa Bintana ◽  
Putri Aisyiyah Rakhma Devi ◽  
Umi Laili Yuhana

The quality of the software can be measured by its return on investment. Factors which may affect the return on investment (ROI) is the tangible factors (such as the cost) dan intangible factors (such as the impact of software to the users or stakeholder). The factor of the software itself are assessed through reviewing, testing, process audit, and performance of software. This paper discusses the consideration of return on investment (ROI) assessment criteria derived from the software and its users. These criteria indicate that the approach may support a rational consideration of all relevant criteria when evaluating software, and shows examples of actual return on investment models. Conducted an analysis of the assessment criteria that affect the return on investment if these criteria have a disproportionate effort that resulted in a return on investment of a software decreased. Index Terms - Assessment criteria, Quality assurance, Return on Investment, Software product


Sign in / Sign up

Export Citation Format

Share Document