scholarly journals Cryopreservation of Aceh Swamp Buffalo (Bubalus bubalis) Semen with Combination of Glycerol and Lactose

2017 ◽  
Vol 9 (3) ◽  
pp. 409
Author(s):  
Kartini Eriani ◽  
Nisa Sari ◽  
Rosnizar Rosnizar ◽  
Dasrul Dasrul ◽  
Suhartono Suhartono ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Membrane Integrity ◽  
Bubalus Bubalis ◽  
Genetic Quality ◽  
Plasma Membrane Integrity ◽  
Randomized Design ◽  
Swamp Buffaloes ◽  
Swamp Buffalo ◽  
Fresh Semen

<p class="IsiAbstrakIndo">Aceh swamp buffaloes<span lang="AR-YE" dir="RTL"> </span><em>(Bubalus bubalis) </em>are decreasing their population and genetic quality. This study was conducted to determine the influence of lactose and glycerol cryoprotectants on spermatozoa of Aceh swamp buffaloes<span lang="AR-YE" dir="RTL"> </span><span lang="EN-GB">after</span><span lang="AR-YE" dir="RTL"> </span>thawing<em>.</em><span lang="AR-YE" dir="RTL"> </span><span lang="EN-GB">This study used completely</span>a factorial randomized design with nine treatments, and five replications. Fresh semen of the Aceh swamp buffalo were diluted by using a combination extender lactose cryoprotectants 0 mM (L<sub>0</sub>), 60 mM (L<sub>60</sub>), 120 mM (L<sub>120</sub>) and glycerol 3% (G<sub>3</sub>), 5% (G<sub>5</sub>), 7% (G<sub>7</sub>) with the equilibration of 4 hours. The results showed that the combination of cryoprotectants L<sub>120</sub>G<sub>7 </sub>influenced significantly (P &lt; 0.05) on the quality of spermatozoa of the Aceh swamp buffalo <em>(B. bubalis)</em><span lang="AR-YE" dir="RTL"> </span><span lang="EN-GB">after</span><span lang="AR-YE" dir="RTL">  </span>thawing<em>.</em><span lang="AR-YE" dir="RTL"> </span><span lang="EN-GB">The percentage of sperm motility L</span><sub>120</sub>G<sub>7</sub> (42.60 ± 1.14); viability L<sub>120</sub>G<sub>7 </sub>(55.00 ± 0.71);  acrosome integrity L<sub>120</sub>G<sub>7</sub> (52.00 ± 0.71); and plasma membrane integrity L<sub>120</sub>G<sub>7</sub><span lang="AR-YE" dir="RTL"> (</span>53.20 ± 1.48).  The combination of lactose cryoprotectants 120 mM (L<sub>120</sub>) and glycerol 7% (G<sub>7</sub>) was the best combination to maintain the quality of spermatozoa of swamp buffalo. This finding could be used to define a policy for the spermatozoa storage of Aceh swamp buffalo to artificial insemination (AI). </p>

scholarly journals Potential of Flamboyant Flower (Delonix regia (Boj. ex Hook) Raf) Extract to Maintain Post-Dilution Quality of Aceh Cattle (Bos taurus indicus) Spermatozoa

2020 ◽  
Vol 151 ◽  
pp. 01044
Author(s):  
Marlina Muhammad ◽  
Kartini Eriani ◽  
Aida Fithri ◽  
Amalia Rusdi ◽  
Hendra Saputra
Keyword(s):  
Artificial Insemination ◽  
Bos Taurus ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Plasma Membrane Integrity ◽  
Flower Extract ◽  
Randomized Design ◽  
Bos Taurus Indicus ◽  
Fresh Semen

The objective of this research was to investigate the effect of flamboyant flower extract as a natural antioxidant on sperm quality of Aceh cattle after dilution and equilibration. Fresh semen was collected from 2 healthy bull aged 3.5-4 years old. The research took place in BIB Laboratory. The method used was CRD (Completed Randomized Design), with 6 treatments and 5 replications each The treatments (m/v) were 0%, 0.05%, 0.10%, 0.15% and 0.20% of flamboyant flower extracts. The method used was CRD (Completed Randomized Design), with 6 treatments and 5 replications each. The treatments (m/v) were 0%, 0.05%, 0.10%, 0.15% and 0.20% of flamboyant flower extracts. The data obtained were analyzed by analysis of variance (ANOVA). Parameters evaluated in this study were motility, viability, plasma membrane integrity, and abnormality. Results showed that there was an insignificant effect (p>0.05) of flamboyant flower extract on the quality of Aceh cattle spermatozoa. The best percentage of motility and viability, 80.0% and 88.4%, respectively, were found at P3 (0.15% flamboyant flower extract). In can be concluded from the research that the addition of 0.15% (m/v) D. regia flower extract was able to maintain sperm quality of Aceh cattle to be used in the artificial insemination program.

scholarly journals KUALITAS SPERMATOZOA ASAL CAPUT, CORPUS, DAN CAUDA EPIDIDIMIS PADA KERBAU RAWA (Bubalus bubalis carabenensis)

2016 ◽  
Vol 3 (3) ◽  
pp. 97
Author(s):  
Muhammad Riyadhi ◽  
Akbar Budiansa ◽  
Muhammad Rizal
Keyword(s):  
Artificial Insemination ◽  
Bubalus Bubalis ◽  
Spermatozoa Motility ◽  
Swamp Buffaloes ◽  
Swamp Buffalo

The purpose of this research was evaluate the quality of spermatozoa concentration in the caput, corpus and cauda of the swamp buffalo epididymis (Bubalus bubalis carabanensis).  Method of this research was to exploration to 13 epididymides of eight swamp buffaloes were obtained from Banjar and Banjarmasin slaughterhouses,evaluated the quality of spermatozoa in caput, corpus and cauda of epididymis.  Quality of collected-spermatozoa including spermatozoa motility, percentage of live spermatozoa, spermatozoa concentration and percentage of abnormality.  Result of this study showed that mean of each of caput spermatozoa motility, percentage of live spermatozoa, spermatozoa concentration and percentage of abnormality; 0%, 45.43% (31.87–72%), 189,62 x106 (40–480 x106) and 56.16 %(44.34–66.53%), corpus ;2.77% (1–9%), 58.73% (45.14 –76%), 152.31 x106 (45 – 345x106), and 47.61 %(23.92 – 60.15%), cauda;53.46% (20 – 70%), 74.32 % (56.68 – 83%), 1,459.62 x106 (825 – 2,340x106), and 34.60%(15.89 –50.04%). In conclusion, spermatozoaofcaudaepididymis could be used in artificial insemination program.Keywords: Spermatozoa, epididymis, swamp buffalo.

scholarly journals The Effect of Glutathione on The Quality of Aceh Local Catfish (Clarias gariepinus) Spermatozoa After Cryopreservation

2020 ◽  
Vol 12 (1) ◽  
pp. 125-131
Author(s):  
Raudhah Mahfudhah ◽  
Kartini Eriani ◽  
Zainal Abidin Muchlisin ◽  
Cut Ruhul Muthmainnah
Keyword(s):  
Plasma Membrane ◽  
Clarias Gariepinus ◽  
Membrane Integrity ◽  
Dna Integrity ◽  
Freezing And Thawing ◽  
Randomized Design ◽  
Fetal Bovine ◽  
Fresh Semen ◽  
Different Doses

The cryopreservation process might reduce the quality of spermatozoa due to an increase in the production of reactive oxygen species (ROS) compounds during cooling, freezing, and thawing. The quality of spermatozoa can be maintained by adding glutathione as an exogenous antioxidant into cryo-diluent. This study aimed to examine the effect of the addition of different doses of glutathione in cryopreservation of Aceh Local catfish (Clarias gariepinus) spermatozoa after freezing. The method used was a completely randomized design (CRD) with four treatments and four replications. Fresh semen was diluted in Ringer, 15% DMSO, and 20% Fetal Bovine Serum (FBS) and then added with glutathione antioxidants of 0.0 mgL-1, 0.5 mgL-1, 1.0 mgL-1, and 2.0 mgL-1. The parameters observed in this study were motility, integrity of the plasma membrane, fertility, and DNA integrity. The results showed that the concentration of glutathione had no effect on motility, integrity ofthe plasma membrane, or fertility, but had an effect on DNA integrity. The highest percentage of motility and plasma membrane integrity respectively was 40.50% (P3) and 70.87% (P2). Furthermore, the assessment of DNA integrity showed that there was no DNA fragmentation both treatments and fresh spermatozoa. This research is the first study regarding glutathione supplementation in cryo-diluent of Aceh Local catfish spermatozoa. Finally, the results obtained can provide information about the exact concentration of glutathione in the extender on the quality of spermatozoa of Aceh Local catfish (C. gariepinus) after the cryopreservation process. These results can also increase the success of fertility be used by the seed hall unit and the aquaculture industry to increase the productivity and supply high quality seeds.

17 EFFECT OF LOCAL TREATMENT OF SEMINAL VESICULITIS ON THE QUALITY OF EQUINE FRESH SEMEN

2015 ◽  
Vol 27 (1) ◽  
pp. 101
Keyword(s):  
Semen Quality ◽  
Local Treatment ◽  
Membrane Integrity ◽  
Ringer Lactate ◽  
Plasma Membrane Integrity ◽  
Progressive Motility ◽  
Seminal Parameters ◽  
Fresh Semen ◽  
Lactate Solution

Stallions affected by seminal vesiculitis present history of infertility or subfertility, ejaculatory disturbance, spread of sexually transmitted pathogens, and changes in semen characteristics, leading to reduced semen quality and longevity. The aim of this study was to evaluate the semen quality of stallions with seminal vesiculitis before and after local treatment. Five stallions with a mean age of 12.4 years diagnosed with seminal vesiculitis were used. The identification of the microorganism involved in the pathogenesis of seminal vesiculitis of each animal was performed by bacterial culture of the seminal vesicles flush with Ringer Lactate solution, performed in duplicate at 1-week intervals. After identification of bacteria was performed, there was susceptibility testing to antibiotic (antibiogram) and the appropriate antibiotic was chosen. The local treatment was performed by endoscopy for 10 consecutive days, and this consisted of flushing with Ringer Lactate solution, followed by infusion of the antibiotic selected. The semen analyses were performed before starting the local treatment for seminal vesiculitis (M0), after a week (M1), and after a month (M2) of therapy. Sperm kinetics were performed by computerized method – CASA for the following parameters: percentage of sperm with total motility, progressive motility, and rapid sperm. Analysis of plasma membrane integrity was performed by epi-fluorescence microscopy, using the combination of fluorescent probes carboxyfluorescein diacetate and propidium iodide. Percentage of leukocytes was assessed through evaluation in light optical microscopy of semen smears stained with DiffQuick. The content of nitric oxide (NO) was determined by colourimetric Griess reaction by a spectrophotometer through the concentrations of nitrate (NO3–) and nitrite (NO2–). To perform the count of colony forming units per millilitre (CFU mL–1), an aliquot of 0.1 mL of semen was diluted in 9.9 mL of saline. A 0.1-mL aliquot of this sample was plated on Mueller-Hinton agar. The seeded plates were incubated, and the bacterial colonies were counted after 24 h. According to the performed dilution, total colonies identified corresponds to ×10 000 CFU mL–1. The data were analysed by two-way ANOVA followed by Tukey's test (P < 0.05). The values (mean ± standard error) of seminal parameters on M0, M1, and M2 were the following, respectively: sperm kinetics (total motility: 46.5 ± 5.13a; 75.1 ± 3.42b; 42.8 ± 5.28a; progressive motility: 19.3 ± 3.86a; 33.4 ± 2.39b; 16.5 ± 2.40a; rapid sperm: 22.2 ± 1.82a; 52.2 ± 5.65b; 22.1 ± 2.62a); plasma membrane integrity (47.5 ± 4.65a; 62.9 ± 5.41b; 39.1 ± 4.32a); percentage of leukocytes (35.2 ± 2.36a; 15.1 ± 2.55b; 36.1 ± 4.04a); CFU (119 980 × 103 ± 19 528.0 × 103a; 5375 × 103 ± 2453.7 × 103b; 65 850 × 103 ± 19 701.0 × 103ab) on fresh semen; and NO content (0.645 ± 0.172a, 0.117 ± 0.023b, 0.364 ± 0.110ab) on seminal plasma. The results demonstrate that local treatment after a week leads to an improvement in sperm quality; however, this was not maintained after 1 month of therapy, since the seminal parameters at this time are similar to pretreatment, which can be justified by recurrent disease.

scholarly journals The Effect of Different Concentrations of Caffeine, Pentoxifylline and 2’-Deoxyadenosine on the Biological Properties of Frozen-Thawed Canine Semen

2019 ◽  
Vol 19 (3) ◽  
pp. 733-746
Author(s):  
Marek Lecewicz ◽  
Rafał Strzeżek ◽  
Anna M. Majewska ◽  
Piotr S. Purpurowicz ◽  
Władysław Kordan
Keyword(s):  
Plasma Membrane ◽  
Sperm Motility ◽  
Artificial Insemination ◽  
Membrane Integrity ◽  
Phosphodiesterase Inhibitors ◽  
Biological Properties ◽  
Kinematic Parameters ◽  
Plasma Membrane Integrity ◽  
Beneficial Effects

AbstractArtificial insemination (AI) and semen cryopreservation are the most accessible and commonly used techniques for breeding domestic animals. Among many parameters, such as plasma membrane integrity and acrosome structure, one of the key factors that determine the quality of frozen-thawed samples for artificial insemination is sperm motility. Sperm motility is one of the key parameters that determine the quality of frozen-thawed samples for AI. The total number of progressively motile spermatozoa in thawed canine semen is correlated with fertility. A variety of substances were used to compare sperm motility with the control. The aim of this study was to determine the effect of semen extender supplementation with motility stimulants, pentoxifylline (PTX), caffeine (CAF) and 2’-deoxyadenosine (DX), after different post-thaw incubation times (30, 60, 120 min) on the motility, selected kinematic parameters, plasma membrane integrity and mitochondrial membrane potential of cryopreserved canine spermatozoa. During attempts to improve the quality of cryopreserved semen, the applied substances exerted beneficial effects at a concentration of 10 mM. We demonstrated that both phosphodiesterase inhibitors, caffeine and pentoxifylline, as well as 2’-deoxyadenosine increased the motility and selected kinematic parameters of thawed canine spermatozoa.

scholarly journals A review chromosome analysis comparison of swamp buffalo (Bubalus bubalis) with different phenotypic characters in East Java, Indonesia

2022 ◽  
Vol 335 ◽  
pp. 00044
Author(s):  
Ardyah Ramadhina Irsanti Putri ◽  
Gatot Ciptadi ◽  
Agus Budiarto ◽  
Sri Rahayu ◽  
Helly Nurul Karima ◽  
...  
Keyword(s):  
Chromosome Analysis ◽  
Bubalus Bubalis ◽  
Breeding Programs ◽  
Genetic Quality ◽  
Phenotypic Characters ◽  
Minimum Number ◽  
Chromosomal Data ◽  
Swamp Buffalo ◽  
And Performance

Chromosomal data on swamp buffalo especially in East Java province, is still lacking. For breeders, chromosomal analysis needs to be done to detect the possibility of hereditary genetic defects. In Malang Area, farmers lack of care and breeding programs for swamp buffalo also caused a decline in the genetic quality and performance. The purpose of this study was to compare the results of the chromosome analysis of swamp buffalo in Malang and another area in East Java province, which were selected based on differences in phenotypic characters. Chromosome analysis is very important for breeders because the results of this study can be used as a consideration for strategies to improve the genetic quality of swamp buffalo. The method used is standard karyotyping using whole blood with G banding staining. Chromosomal preparation using Karyo MAX medium, Colcemic Solution, Giemsa Stain, and KCl solution. Cell culture was performed according to the standard karyotyping method in mammals. The minimum number of 5 spreading chromosomes is the best was chosen, microphotographed, and then chromosome analysis is performed using Cytovision Image Analysis software. The results of this study did not find swamp buffalo with an abnormal number of chromosomes in East Java Province, Indonesia.

The effects of Trolox on the quality of sperm from captive squirrel monkey during liquefaction in the extender ACP-118™

Zygote ◽  
2018 ◽  
Vol 26 (4) ◽  
pp. 333-335 ◽  
Author(s):  
D. V. C. Almeida ◽  
J. S. Lima ◽  
D. L. Leão ◽  
K. G. Oliveira ◽  
R. R. Santos ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Squirrel Monkey ◽  
Sperm Motility ◽  
Membrane Integrity ◽  
Plasma Membrane Integrity ◽  
Time Periods ◽  
Semen Extender ◽  
Fresh Semen

SummaryThe aim of this study was to evaluate the effect of incubating semen for different periods (90, 270 or 450 min) with or without Trolox® (100 or 150 µM) on the quality of sperm from Saimiri collinsi. Sperm motility, vigour, and plasma membrane integrity (PMI) were evaluated in both fresh semen and semen incubated for different time periods, i.e. 90, 270 or 450 min of incubation. Supplementation of semen extender with Trolox® 100 µM improved sperm motility, vigour and PMI for up to 270 min of incubation.

scholarly journals Membrane Integrity Test for Spermatozoa in Simmental Bull Liquid Semen Using the Addition of Gentamycin and Sweet Orange Essential Oil in the Extender

2020 ◽  
pp. 42-46
Author(s):  
Sukma Aditya Sitepu ◽  
Julia Marisa
Keyword(s):  
Essential Oil ◽  
Sweet Orange ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Integrity Test ◽  
Randomized Design ◽  
Simmental Cattle ◽  
Fresh Semen ◽  
Orange Essential Oil

Aims: This study aimed to determine the quality of Simmental Bull liquid semen through laboratory tests, including the percentage value of membrane integrity Spermatozoa with the addition of gentamycin and sweet orange essential oil to tris yolk extender. Methodology: The material used in this research is Simmental Cattle bull fresh semen, tris yolk extender, gentamicin, and sweet orange essential oil. Tris yolk extender was prepared with Tris (hydroxymethyl aminomethane) (3.32 g), citric acid (1.86 g), fructose (1.37 g), glycerol (6 ml), egg yolk (20 ml), aquades (100 ml). The experimental design used in the study was a nonfactorial Complete Randomized Design with five treatments and five replicates. The treatment given is the addition of sweet orange essential oil 0%, 0.25%, 0.5%, 0.75% and 1%. The parameter observed was the evaluation of membrane integrity before equilibration and after equilibration. Results: The results showed that the percentage of membrane integrity in Simmental bull liquid semen continues to increase with the addition of sweet orange essential oils. The results showed that the addition of sweet orange essential oil as much as 0% produced a membrane integrity percentage after equilibration of 63%, adding 0.25% resulted in 68%, adding 0.5% yielded 70%, adding 0.75% yielded 75% and adding 1% resulted in 79%. Conclusion: The best percentage of membrane integrity in Simmental bull liquid semen was the addition of gentamicin and sweet orange essential oil in the tris yolk extender at the level of 1%.

scholarly journals Quality of Sexed Sperm of Bali Bull in Regional Artificial Insemination Center of Riau Province

2019 ◽  
Vol 43 (2) ◽  
Author(s):  
Yendraliza Yendraliza ◽  
Anwar Efendi Harap ◽  
July Handoko ◽  
Muhammad Rodiallah
Keyword(s):  
Plasma Membrane ◽  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Female Offspring ◽  
Plasma Membrane Integrity ◽  
Frozen Semen ◽  
Calving Rate

This study aimed to evaluate the quality of frozen semen of Bali bull resulted from sexing procedure on calf or offspring production with desired sex. The tested sperm of Bali bull were collected from Bali bull raised at Regional Artificial Insemination Center of Riau Province (BIBD Riau). The study was carried out in 2 stages. The first stage was X and Y chromosome separation by albumin method. The extender used in the sexing procedure is trice citrate fructose and egg yolk. The second stage was mainly testing the sexed sperm collected in 60 Bali cow in Langkat Village, Bengkalis Regency. To determine the quality of post thawing frozen semen collected from the sexing procedure, the study evaluated motility, viability, mortality, abnormality and plasma membrane integrity of the spermatozoa. The pregnancy rate, calving rate, and birth accuracy of inseminated sexed sperm to offspring’ sex were also evaluated. The evaluation resulted in motility (66.3-75.3%), viability (70-78.5%), plasma membrane integrity (60-65.8%), abnormality (6.05-8.05%), mortality (20.05-30.05%), and pregnancy rate (83.33-90%). The calving rate on this study was 100% with the birth accuracy of 81.8% for male offspring and 40% for female offspring. As conclusion, the sexed sperm evaluated on this study have fairly good fertility.

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