Butylated hydroxytoluene inclusion in semen extender improves the post-thawed semen quality of Nili-Ravi buffalo (Bubalus bubalis)

The study was conducted to evaluate the potential cryoprotective effect of butylated hydroxytoluene (BHT) through post-thaw evaluation of canine semen and its optimal inclusion level. Ejaculated canine semen was extended in TRIS-glucose egg yolk extender containing various concentrations of BHT (0.5, 1.0, 1.5, 2.0, and 2.5mM). Semen was frozen at −196°C using 200 × 10<sup>6 </sup>spermatozoa per 0.5 ml straws and post-thaw evaluation was carried out in terms of sperm motility, viability, plasma membrane integrity, and acrosomal integrity through phase-contrast microscope, supravital staining, hypo-osmotic swelling test, and normal acrosomal ridge, respectively. BHT was found to improve (P > 0.005) all post-thawed semen quality parameters at an inclusion level of 1.0mM in the extended semen. However, higher concentrations than this were found to have detrimental effects.  

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ADDITION OF ALPHA-LIPOIC ACID IN SEMEN EXTENDER IMPROVES POST-THAW ANTIOXIDANT PROFILE AND SEMEN QUALITY OF ACHAI (BOS INDICUS) CATTLE BULLS

The Journal of Animal and Plant Sciences - February ◽

10.36899/japs.2021.3.0254 ◽

2020 ◽

Vol 31 (3) ◽

Keyword(s):

Lipoic Acid ◽

Semen Quality ◽

Bos Indicus ◽

Alpha Lipoic Acid ◽

Antioxidant Profile ◽

Semen Extender

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Effects of Different Concentrations of L-Carnitine in Lecithin-Based Semen Extender on Semen Quality of Ghezel Ram after Freeze Thawing Process

Research on Animal Production ◽

10.29252/rap.9.19.48 ◽

2018 ◽

Vol 9 (19) ◽

pp. 48-53

Author(s):

Hossein Daghigh Kia ◽

Sepehr Jafari ◽

Gholamali Moghaddam ◽

Marziyeh Ebrahimi ◽

Abozar Najafi ◽

...

Keyword(s):

Semen Quality ◽

Thawing Process ◽

Semen Extender

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Protective effect of natural antioxidant Opuntia ficus-indica on ram semen quality stored at 5 °C for 72

10.21203/rs.3.rs-522174/v1 ◽

2021 ◽

Author(s):

Larbi ALLAI ◽

Xavier Druart ◽

Pinar Terzioğlue ◽

Noureddine Louanjli ◽

Boubker Nasser ◽

...

Keyword(s):

Antioxidant Activity ◽

Artificial Insemination ◽

Semen Quality ◽

Skim Milk ◽

Butylated Hydroxytoluene ◽

Emission Spectrometry ◽

Control Group ◽

Opuntia Ficus Indica ◽

Analytical Instruments

Abstract Background Recently, researchers have focused on the use of natural antioxidants to improve semen quality as a key element for successful artificial insemination. In this context the first aim of this study was to determine the antioxidant activity and composition (minerals, vitamins, and sugars) of Opuntia ficus-indica cladode ethanolic extract (ETHEX). A further purpose of the study was to investigate the effect of ETHEX supplementation on the quality of liquid ram semen extended with skim milk (SM) at 5°C. The antioxidant activity of ETHEX was studied using DPPH• assay. The mineral composition and the sugar and vitamin contents of ETHEX were determined using an inductively coupled plasma optical emission spectrometry (ICP-OES), and HPLC-DAD-RID analytical instruments. As a second part, semen was collected from five Boujaâd rams with an artificial vagina. The ejaculates with more than 70 % motility were pooled, extended with skim milk (SM) extender without (control) or supplemented with 1–8 % of ETHEX (37°C; 0.8 × 109 sperm/mL). Sperm quality parameters were assessed at 8, 24, 48 and 72 h. Results The results showed that ETHEX had a higher antioxidant activity compared to those of ascorbic acid and butylated hydroxytoluene (BHT). Furthermore, ETHEX contains a considerable amount of minerals, vitamins, and sugars. The inclusion of 1 or 2 % ETHEX in SM increased the sperm motility, viability, membrane integrity and decreased the abnormality, spontaneous and catalyzed lipids peroxidation (P < 0.05) up to 72 h. In addition, semen diluted with 1 and 2 % ETHEX decreased the level of DNA fragmentation compared to the control group (P < 0.05). Conclusions In conclusion the ETHEX could be recommended to improve the quality of liquid ram spermatozoa. However, its effects on sperm physiology and artificial insemination should be further studied.

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Identifikasi Bakteri dan Efektivitas Antibiotik dalam Pengencer untuk Mengontrol Pertumbuhan Bakteri pada Semen Sapi Friesian Holstein

jurnal veteriner ◽

10.19087/jveteriner.2019.20.1.140 ◽

2019 ◽

Vol 20 (1) ◽

pp. 140

Author(s):

Muttaqinullah Rabusin ◽

Andriani Andriani ◽

Raden Iis Arifiantini ◽

Ni Wayan Kurniani Karja

Keyword(s):

Semen Quality ◽

Bacterial Species ◽

Enterobacter Cloacae ◽

Bovine Semen ◽

Frozen Semen ◽

Bacterial Content ◽

Pseudomonas Diminuta ◽

Semen Extender ◽

Fresh Semen

This study was designed to investigate the presence of bacterial species in Friesian Holstein (FH) bovine semen at the time of collection, processing and to assess the efficacy of two types of antibiotics combinations; penicillin and streptomycin (PS) and gentamycin, tylosin, lincomycin and spectinomycin (GTLS) in semen extender on bacterial control and quality of semen. For this purpose, three experiments were conducted. In experiment 1, identification of bacterial content in fresh semen which collected from 5 bovine ejaculates. In experiment 2, identification of bacterial content in skimmilk-eggyolk extender which were prepared in artificial insemination center, Lembang, Bandung. In experiment 3, identification of bacterial content in frozen thawed semen. In the result, some of bacterial species were isolated from the bovine semen. The GTLS combination of antibiotics may be incorporated into a freezing extender or protocol without compromising the post-thawed semen quality of FH bull spermatozoa. Three types of bacteria were found in fresh semen; Klebsiella sp., Micrococcus sp., and Pediococcus sp.. Three types of bacteria were found in semen extender; Enterobacter cloacae, Pseudomonas diminuta and Serratia plymutica. Two types of bacteria were found in frozen semen; Enterobacter cloacae and Serratia plymutica. In conclusion, antibiotics PS and GTLS were effective for controlling the growth of bacteria in frozen semen.

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Effects of the Seminal Plasma Zinc Content and Catalase Activity on the Semen Quality of Water Buffalo (Bubalus bubalis) Bulls

Pakistan Journal of Biological Sciences ◽

10.3923/pjbs.2009.134.139 ◽

2009 ◽

Vol 12 (2) ◽

pp. 134-139 ◽

Cited By ~ 8

Author(s):

S.M. Alavi-Shou ◽

S. Asri Rezai ◽

M.H. Kh. Ansari ◽

A. Khaki

Keyword(s):

Seminal Plasma ◽

Catalase Activity ◽

Water Buffalo ◽

Semen Quality ◽

Zinc Content ◽

Bubalus Bubalis ◽

Plasma Zinc ◽

Quality Of Water

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PSV-9 Effects of Natural Honey Inclusion in Dilution and Freezing Extenders on Frozen-thawed Semen Quality in Boars

Journal of Animal Science ◽

10.1093/jas/skab054.346 ◽

2021 ◽

Vol 99 (Supplement_1) ◽

pp. 211-211

Author(s):

Kayode B Balogun ◽

Griffin Nicholls ◽

Olujide Sokunbi ◽

Kara R Stewart

Keyword(s):

Semen Quality ◽

Swine Industry ◽

Normal Cells ◽

Progressive Motility ◽

Normal Sperm ◽

Frozen Semen ◽

Boar Semen ◽

Semen Extender ◽

Natural Honey

Abstract Improvements in the post-thaw quality of frozen semen could allow increased utilization of this technology in the swine industry. The objective of this study was to investigate the effects of natural honey inclusion in semen extender and freezing media on motility, mobility, and morphology of cryopreserved boar sperm. Ejaculates from 6 terminal cross-bred boars were collected using the gloved-hand technique for 3 weeks and used in a 2 x 3 factorial study design. Following collection, semen samples were incubated overnight in dilution extender with and without natural honey (D0: Androhep Plus; D1: Androhep Plus + 0.25% honey). The following day, the semen samples were cooled to 4 C in LEY cooling extender then frozen in freezing media containing 93% cooling extender + 6% glycerol + 1% Equex-STM Paste (F1), or freezing media with natural honey replacing 50% of the glycerol (F2) or 50% of the Equex-STM paste (F3). Semen samples were frozen using a controlled-rate freezer and stored in liquid nitrogen. Two straws per treatment for each boar were thawed and semen quality assessed. The inclusion of natural honey in dilution extender had no effect on post-thaw motility (P=0.733), progressive motility (P=0.562), or other mobility parameters (0.995≤P≥0.081). However, D1 had a higher percentage of normal acrosomes (P=0.001) and morphologically normal cells (P< 0.001) resulting from lower tail abnormalities compared to D0 (P=0.006). Post-thaw motility (P< 0.001) and progressive motility (P< 0.001) were increased in F3 compared to both F2 and F1. F1 had reduced normal acrosomes (P=0.009) and morphologically normal cells (P< 0.001) resulting from higher tail abnormalities (P< 0.001). In conclusion, the inclusion of natural honey, at 0.25%, in dilution extender helps maintain normal sperm and acrosome morphology, and replacing 50% Equex-STM Paste with honey in freezing extender improves post-thaw sperm motility and progressive motility of frozen-thawed boar semen.

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