KUALITAS SPERMATOZOA ASAL CAPUT, CORPUS, DAN CAUDA EPIDIDIMIS PADA KERBAU RAWA (Bubalus bubalis carabenensis)

The purpose of this research was evaluate the quality of spermatozoa concentration in the caput, corpus and cauda of the swamp buffalo epididymis (Bubalus bubalis carabanensis). Method of this research was to exploration to 13 epididymides of eight swamp buffaloes were obtained from Banjar and Banjarmasin slaughterhouses,evaluated the quality of spermatozoa in caput, corpus and cauda of epididymis. Quality of collected-spermatozoa including spermatozoa motility, percentage of live spermatozoa, spermatozoa concentration and percentage of abnormality. Result of this study showed that mean of each of caput spermatozoa motility, percentage of live spermatozoa, spermatozoa concentration and percentage of abnormality; 0%, 45.43% (31.87–72%), 189,62 x106 (40–480 x106) and 56.16 %(44.34–66.53%), corpus ;2.77% (1–9%), 58.73% (45.14 –76%), 152.31 x106 (45 – 345x106), and 47.61 %(23.92 – 60.15%), cauda;53.46% (20 – 70%), 74.32 % (56.68 – 83%), 1,459.62 x106 (825 – 2,340x106), and 34.60%(15.89 –50.04%). In conclusion, spermatozoaofcaudaepididymis could be used in artificial insemination program.Keywords: Spermatozoa, epididymis, swamp buffalo.

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Cryopreservation of Aceh Swamp Buffalo (Bubalus bubalis) Semen with Combination of Glycerol and Lactose

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v9i3.11426 ◽

2017 ◽

Vol 9 (3) ◽

pp. 409

Author(s):

Kartini Eriani ◽

Nisa Sari ◽

Rosnizar Rosnizar ◽

Dasrul Dasrul ◽

Suhartono Suhartono ◽

...

Keyword(s):

Artificial Insemination ◽

Membrane Integrity ◽

Bubalus Bubalis ◽

Genetic Quality ◽

Plasma Membrane Integrity ◽

Randomized Design ◽

Swamp Buffaloes ◽

Swamp Buffalo ◽

Fresh Semen

Aceh swamp buffaloes (Bubalus bubalis) are decreasing their population and genetic quality. This study was conducted to determine the influence of lactose and glycerol cryoprotectants on spermatozoa of Aceh swamp buffaloes after thawing. This study used completelya factorial randomized design with nine treatments, and five replications. Fresh semen of the Aceh swamp buffalo were diluted by using a combination extender lactose cryoprotectants 0 mM (L0), 60 mM (L60), 120 mM (L120) and glycerol 3% (G3), 5% (G5), 7% (G7) with the equilibration of 4 hours. The results showed that the combination of cryoprotectants L120G7 influenced significantly (P < 0.05) on the quality of spermatozoa of the Aceh swamp buffalo (B. bubalis) after thawing. The percentage of sperm motility L120G7 (42.60 ± 1.14); viability L120G7 (55.00 ± 0.71); acrosome integrity L120G7 (52.00 ± 0.71); and plasma membrane integrity L120G7 (53.20 ± 1.48). The combination of lactose cryoprotectants 120 mM (L120) and glycerol 7% (G7) was the best combination to maintain the quality of spermatozoa of swamp buffalo. This finding could be used to define a policy for the spermatozoa storage of Aceh swamp buffalo to artificial insemination (AI).

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A review chromosome analysis comparison of swamp buffalo (Bubalus bubalis) with different phenotypic characters in East Java, Indonesia

E3S Web of Conferences ◽

10.1051/e3sconf/202233500044 ◽

2022 ◽

Vol 335 ◽

pp. 00044

Author(s):

Ardyah Ramadhina Irsanti Putri ◽

Gatot Ciptadi ◽

Agus Budiarto ◽

Sri Rahayu ◽

Helly Nurul Karima ◽

...

Keyword(s):

Chromosome Analysis ◽

Bubalus Bubalis ◽

Breeding Programs ◽

Genetic Quality ◽

Phenotypic Characters ◽

Minimum Number ◽

Chromosomal Data ◽

Swamp Buffalo ◽

And Performance

Chromosomal data on swamp buffalo especially in East Java province, is still lacking. For breeders, chromosomal analysis needs to be done to detect the possibility of hereditary genetic defects. In Malang Area, farmers lack of care and breeding programs for swamp buffalo also caused a decline in the genetic quality and performance. The purpose of this study was to compare the results of the chromosome analysis of swamp buffalo in Malang and another area in East Java province, which were selected based on differences in phenotypic characters. Chromosome analysis is very important for breeders because the results of this study can be used as a consideration for strategies to improve the genetic quality of swamp buffalo. The method used is standard karyotyping using whole blood with G banding staining. Chromosomal preparation using Karyo MAX medium, Colcemic Solution, Giemsa Stain, and KCl solution. Cell culture was performed according to the standard karyotyping method in mammals. The minimum number of 5 spreading chromosomes is the best was chosen, microphotographed, and then chromosome analysis is performed using Cytovision Image Analysis software. The results of this study did not find swamp buffalo with an abnormal number of chromosomes in East Java Province, Indonesia.

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Carcass Characteristics and Meat Quality of Swamp Buffaloes (<italic>Bubalus bubalis</italic>) Fattened at Different Feeding Intensities

Asian-Australasian Journal of Animal Sciences ◽

10.5713/ajas.2013.13555 ◽

2014 ◽

Vol 27 (4) ◽

pp. 551-560 ◽

Cited By ~ 3

Author(s):

C. Lambertz ◽

P. Panprasert ◽

W. Holtz ◽

E. Moors ◽

S. Jaturasitha ◽

...

Keyword(s):

Meat Quality ◽

Carcass Characteristics ◽

Bubalus Bubalis ◽

Swamp Buffaloes

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Seasonal variation in semen quality of swamp buffalo bulls (Bubalus bubalis) in Thailand

Asian Journal of Andrology ◽

10.1111/j.1745-7262.2007.00230.x ◽

2007 ◽

Vol 9 (1) ◽

pp. 92-101 ◽

Cited By ~ 13

Author(s):

Seri Koonjaenak ◽

Vichai Chanatinart ◽

Suneerat Aiumlamai ◽

Tanu Pinyopumimintr ◽

Heriberto Rodriguez-Martinez

Keyword(s):

Seasonal Variation ◽

Semen Quality ◽

Bubalus Bubalis ◽

Swamp Buffalo

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Age-Dependent Changes in Fresh Semen Quality of Swamp Buffalo (Bubalus bubalis)

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/478/1/012034 ◽

2020 ◽

Vol 478 ◽

pp. 012034

Author(s):

N Isnaini ◽

T Harsi ◽

W R Zamani

Keyword(s):

Semen Quality ◽

Bubalus Bubalis ◽

Age Dependent ◽

Swamp Buffalo ◽

Fresh Semen

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The distribution of runs of homozygosity in the genome of river and swamp buffaloes reveals a history of adaptation, migration and crossbred events

Genetics Selection Evolution ◽

10.1186/s12711-021-00616-3 ◽

2021 ◽

Vol 53 (1) ◽

Author(s):

Nicolo P. P. Macciotta ◽

Licia Colli ◽

Alberto Cesarani ◽

Paolo Ajmone-Marsan ◽

Wai Y. Low ◽

...

Keyword(s):

Water Buffalo ◽

Bubalus Bubalis ◽

River Buffalo ◽

Chromosome 2 ◽

Runs Of Homozygosity ◽

Data Set ◽

Swamp Buffaloes ◽

History Of ◽

Swamp Buffalo ◽

Genomic Inbreeding

Abstract Background Water buffalo is one of the most important livestock species in the world. Two types of water buffalo exist: river buffalo (Bubalus bubalis bubalis) and swamp buffalo (Bubalus bubalis carabanensis). The buffalo genome has been recently sequenced, and thus a new 90 K single nucleotide polymorphism (SNP) bead chip has been developed. In this study, we investigated the genomic population structure and the level of inbreeding of 185 river and 153 swamp buffaloes using runs of homozygosity (ROH). Analyses were carried out jointly and separately for the two buffalo types. Results The SNP bead chip detected in swamp about one-third of the SNPs identified in the river type. In total, 18,116 ROH were detected in the combined data set (17,784 SNPs), and 16,251 of these were unique. ROH were present in both buffalo types mostly detected (~ 59%) in swamp buffalo. The number of ROH per animal was larger and genomic inbreeding was higher in swamp than river buffalo. In the separated datasets (46,891 and 17,690 SNPs for river and swamp type, respectively), 19,760 and 10,581 ROH were found in river and swamp, respectively. The genes that map to the ROH islands are associated with the adaptation to the environment, fitness traits and reproduction. Conclusions Analysis of ROH features in the genome of the two water buffalo types allowed their genomic characterization and highlighted differences between buffalo types and between breeds. A large ROH island on chromosome 2 was shared between river and swamp buffaloes and contained genes that are involved in environmental adaptation and reproduction.

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Identification of growth genes diversity of swamp buffalo using RFLP in Kabaena Island, Bombana District, Southeast Sulawesi, Indonesia

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d210516 ◽

2020 ◽

Vol 21 (5) ◽

Author(s):

La Ode Nafiu ◽

Muzuni ◽

Muhammad Pagala ◽

Widhi Kurniawan ◽

Syam Rahadi

Keyword(s):

Bubalus Bubalis ◽

Chi Square ◽

The Public ◽

Weinberg Equilibrium ◽

Genotype Frequencies ◽

Swamp Buffaloes ◽

Hardy Weinberg Equilibrium ◽

Swamp Buffalo ◽

The Government ◽

Chi Square Analysis

Abstract. Nafiu LO, Muzuni, Pagala MA, Kurniawan W, Rahadi S. 2020. Identification of growth genes diversity of swamp buffalo using RFLP in Kabaena Island, Bombana District, Southeast Sulawesi, Indonesia. Biodiversitas 21: 1901-1907. Swamp buffalo (Bubalus bubalis) in Bombana District has been familiar in the socio-cultural life and used as a source of livelihood. Buffalo can adapt to the hard environment by utilizing a low-quality feed. However, it needs more attention from the public and the government to increase buffalo production, both from the genetic and environmental aspects. The objective of this study was to determine the diversity of swamp buffalo growth genes (GH and GHRH) in Kabaena Island, Bombana District, Southeast Sulawesi-Indonesia. The blood sample was taken from 58 heads of swamp buffaloes and analyzed using PCR technique to multiply the sequence of GH and GHRH genes with the target sizes of 327 bp and 451 bp. The Genes diversity determined using analysis of genotype frequencies and allele frequencies of each locus, Inbreeding Coefficient estimated using analysis of observed heterozygosity (Ho) and expected heterozygosity (He), while Population balance (Hardy-Weinberg equilibrium) specifically related to the presence/absence of selection determined using a chi-square analysis. The results of the study showed that the GH/MspI and GHRH/HaeIII locus were polymorphic with sizes of 327 bp and 451 bp, respectively, contain three genotypes; AA, AB, and BB. The frequency of GH/MspI locus A and B locus were 0.562 and 0.438, respectively. Meanwhile, the frequency of A and B alleles at the GHRH/HaeIII locus were0.700 and 0.300, respectively. Allele and genotype GH/ MspI - GHRH/HaeIII locus frequency of swamp buffalo in Kabaena Island, Bombana District were in Hardy-Weinberg equilibrium, and it means that mating tends to occur randomly.

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Evaluasi Kualitas Spermatozoa Ayam Brahma, Cochin dan Bangkok Sebagai Dasar Penerapan Teknologi Inseminasi Buatan pada Ayam Kampung

Jurnal Sains Teknologi & Lingkungan ◽

10.29303/jstl.v0i0.250 ◽

2021 ◽

pp. 1-9

Author(s):

Asnawi Asnawi ◽

Budi Indarsih ◽

Dewi Haryani ◽

Sukartha Jaya ◽

Maya Nachida ◽

...

Keyword(s):

Artificial Insemination ◽

Sperm Quality ◽

Animal Science ◽

Spermatozoa Motility ◽

Animal Reproduction ◽

Randomized Design ◽

Completely Randomized Design ◽

Artificial Vagina ◽

Local Chickens

Research on the evaluation of the spermatozoa quality of Brahma, Cochin and Bangkok chickens as the basis for the application of artificial insemination technology in native chickens has been carried out. The purpose of this study was to evaluate the spermatozoa quality of Brahma, Cochin and Bangkok rooster as the basis for determining the spermatozoa dose for artificial insemination in local chickens. method research was used a completely randomized design (CRD) with three treatments, that is: Brahma, Cochin and Bangkok rooster. Each treatment was repeated five times. Cement storage is carried out by inserting an artificial vagina into the cloaca of the rooster and stimulating it to climb the hen. The collected sperm were analyzed at the Animal Reproduction Laboratory, Faculty of Animal Science, Mataram University. The variables observed included motility, viability and morphology of spermatozoa. The results of studied that the spermatozoa motility of Brahma, Chicin and Bangkok rooster were 72 ± 2.74%. 74 ± 2.24% and 73 ± 2.74% (P> 0.05) respectively; The spermatozoan viability of Brahma rooster and Bangkok rooster was 99.2 ± 1.30%, 99.4 ± 0.55% and 99.2 ± 1.10% (P>0.05)respectively. The spermatozoan morphology of brahma rooster, chicin rooster and Bangkok rooster was 94.6 ± 2.30%, 94.4 ± 2.70% and 97.4 ± 2.07% (P> 0.05), respectively. Cochin rooster and Bangkok rooster respectively, 62.6 ± 7.92 x 107, 57 ± 5.83 x107 , and 65.2 ± 12.28 x107, respectively. The results of the study concluded that Brahma, Cochin and Bangkok rooster had good sperm quality and could be able to used in artificial insemination of Kampung hens.

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PENGARUH FALSE MOUNTING TERHADAP KUALITAS SEMEN SAPI LIMOUSIN DAN SIMMENTAL DI BALAI INSEMINASI BUATAN LEMBANG

ZOOTEC ◽

10.35792/zot.39.2.2019.25749 ◽

2019 ◽

Vol 39 (2) ◽

pp. 417

Author(s):

Yuli Siska Kafiar ◽

Sri Adiani ◽

Agustinus Lomboan ◽

H.F.N. Lapian

Keyword(s):

Artificial Insemination ◽

Semen Quality ◽

Mass Movement ◽

T Test ◽

Average Volume ◽

Concentration Data ◽

Spermatozoa Motility ◽

Bull Semen ◽

Semen Volume

THE EFFECT OF FALSE MOUNTING ON SEMEN QUALITY OF LIMOUSIN AND SIMMENTAL BULL IN LEMBANG ARTIFICAL INSEMINATION CENTER. The purpose of this study was to determine the effect of false mounting on the quality of Limosin and Simental bull semen at the Lembang Artificial Insemination Center. The 55 individu of Limousin and 55 Simmental bull aging 5-9 years old have been used in thos study. The data have been collected directly in the Artificial Insemination Center Lembang - West Java Province. The data obtained in the form of macroscopic and microscopic observations on Limousin and Simmental bull include volume, pH, mass movement, spermatozoa motility and spermatozoa concentration. Data were analyzed descriptively by calculating the standard deviations, correlations coefficients and the T test. The variables measured in this study were macroscopic and microscopic quality of semen. The results of this study indicate that the effect of false mounting on the average semen volume of Limousin and Simmental bull is maximum at 3, 4, and 5 times false mounting but there was not significantly difference between the amount of false mounting, as well as the average volume, pH, mass movement, motility of spemetozoa and concentration spermatozoa are not significantly different. It was concluded that false mounting in Limousin and Simmental bulls in Lembang Artificial Insemination Center had no significantly effect on the quality of macroscopic and microscopic semen. Keywords: False Mounting, Cement Quality, Limousin Cattle, Simmental Cows.

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Pregnancy and calving rates following transfer of in-vitro-produced river and F1 (river × swamp) buffalo (Bubalus bubalis) embryos in recipients on natural oestrus or synchronised for ovulation

Reproduction Fertility and Development ◽

10.1071/rd07048 ◽

2007 ◽

Vol 19 (5) ◽

pp. 670 ◽

Cited By ~ 9

Author(s):

Xianwei Liang ◽

Xiufang Zhang ◽

Bingzhuang Yang ◽

Mingtang Cheng ◽

Fenxiang Huang ◽

...

Keyword(s):

Bubalus Bubalis ◽

Sperm Cells ◽

In Vitro Production ◽

In Vitro Development ◽

Antral Follicles ◽

Swamp Buffaloes ◽

Swamp Buffalo ◽

Vitro Development ◽

Vitro Production

The main objective of this study was to compare pregnancy and calving rates following transfer of in-vitro-produced fresh river and F1 (river × swamp) buffalo embryos in recipients synchronised by Ovsynch protocol or following natural oestrus. River embryos were produced from cumulus–oocyte complexes (COCs) derived by ovum pick up (OPU) on 40 Murrah and Nili-Ravi donor buffaloes over a twice-weekly collection schedule for 120 single OPUs. F1 embryos were produced by fertilisation of swamp COCs recovered from abattoir ovaries coincubated with river sperm cells. Both groups of embryos were produced following the same protocol for in vitro production. With regard to the OPU source of COCs, 923 antral follicles were punctured and 647 COCs were recovered (70%). From 462 selected COCs for IVM, 257 (55.6%) cleaved zygotes were recorded leading to 93 blastocysts (20.1%). In total, 590 swamp COCs were aspirated from abattoir ovaries and 476 were selected for IVM leading to 270 (56.7%) cleaved zygotes and resulting in 137 blastocysts (28.8%). River and F1 embryos were transferred between Day 6 to 7 of in vitro development, corresponding to blastocyst–expanding blastocyst, into F1 recipients synchronised by Ovsynch and swamp buffaloes following natural oestrus, respectively, each of them receiving two embryos. According to palpation per rectum of the ovaries at the time of embryo transfer, 26 of the 47 (55.3%) F1 recipients synchronised by Ovsynch were considered suitable for transfer, resulting in seven pregnancies (26.9%) and four calvings (15.3%) owing to three abortions occurring between 2 and 3 months of pregnancy. In total, 29 swamp recipients following natural oestrus were judged suitable as recipients, resulting in 12 pregnancies (41.4%) and 10 calvings (34.5%) owing to two abortions at 2 and 3 months of gestation respectively. Pregnancy and calving rates following transfer of river and F1 embryos were similar. Likewise, weight at birth of calves derived from transfer of river and F1 embryos was not different: 30.5 ± 1.4 and 32.9 ± 2.4 respectively. Pregnancy and calving rates following AI in a group of river and swamp buffaloes considered for reference in this study were similar to recipients carrying in-vitro-produced embryos. Collectively, no apparent postnatal complications were recorded in resulting live calves.

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