Studies on the Elimination of Exogenous Lipids from the Blood Stream. Determination and Separation of the Plasma Triglycerides after Single Injection of a Fat Emulsion in Man

1964 ◽  
Vol 62 (4) ◽  
pp. 407-421 ◽  
Author(s):  
Dag Hallberg
Keyword(s):  
Single Injection ◽  
Blood Stream ◽  
Fat Emulsion ◽  
Plasma Triglycerides

Decreased Oxygenation and Hyperlipemia During Intravenous Fat Infusions in Premature Infants

PEDIATRICS ◽  
1980 ◽  
Vol 66 (1) ◽  
pp. 26-30 ◽  
Author(s):  
Gilberto R. Pereira ◽  
William W. Fox ◽  
Charles A. Stanley ◽  
Lester Baker ◽  
Jacob G. Schwartz
Keyword(s):  
Body Weight ◽  
Pulmonary Function ◽  
Premature Infants ◽  
Gestational Age ◽  
Fat Emulsion ◽  
Plasma Triglycerides ◽  
Appropriate For Gestational Age ◽  
Fat Infusion ◽  
Infusion Period

Eighteen appropriate-for-gestational-age premature infants with birth weights ranging from 0.77 to 1.89 kg received 1 gm/kg of body weight of fat emulsion, intravenously, over a four-hour period. Infants less than 1 week of age developed a significant decrease in Po2 levels (P < .05) during the fat infusion period. There were no changes in other pulmonary function parameters. Infants less than 1 week of age also developed significantly higher peak levels of plasma triglycerides than infants 2 to 3 weeks old (P < .05). A correlation between increment in triglyceride levels and postnatal age was demonstrated (r = .75), with the younger infants presenting the higher triglyceride levels. This study demonstrates that: (1) small premature infants receiving intravenous fat are more susceptible to hyperlipemia and hypoxemia during the first week of life; (2) hypoxemia associated with intravenous fat infusion does not result from changes in lung dynamics; (3) the capacity to tolerate intravenous fats is enhanced after the first week of life.

scholarly journals Magnitude Of Rise In Proneurotensin Is Related To Amount Of Triglyceride Appearance In Blood After A Standardized Oral Intake Of Both Saturated And Unsaturated Fat

2020 ◽  
Author(s):  
Ayesha Fawad ◽  
Celine Fernandez ◽  
Andreas Bergmann ◽  
Joachim Struck ◽  
Peter M Nilsson ◽  
...  
Keyword(s):  
Olive Oil ◽  
Animal Studies ◽  
Venous Blood ◽  
Area Under The Curve ◽  
Oral Intake ◽  
Random Order ◽  
Blood Stream ◽  
Maximum Increase ◽  
Plasma Triglycerides ◽  
Unsaturated Fat

Abstract Background: In rodents, neurotensin contributes to high fat diet induced obesity by facilitation of intestinal fat absorption. The effect of oral lipid load on plasma proneurotensin and relationship with plasma triglycerides in humans is unknown.Aim: To investigate the acute effects of an oral lipid load (including both cream and olive oil) on proneurotensin and plasma triglycerides and their interrelationships in healthy individuals.Setting/ Methods: Twenty-two healthy subjects were given 150 ml of full milk cream (54g fat) and 59 ml of pure olive oil (54 g fat) in the fasted state at two different occasions separated by at least 1 week in random order. Venous blood samples were drawn at fasting,and hourly up to 4 hours after fat ingestion. We compared post-ingestion values of proneurotensin and plasma triglycerides with fasting levels. Further, post ingestion Area Under the Curve (AUC) of proneurotensin was correlated with the AUC of plasma triglycerides.Results: Rise of plasma proneurotensin and plasma triglycerides were observed after ingestion of cream with maximum increase at 2 hours for proneurotensin [mean (95% confidence interval)] of 22 (12-31) pmol/L (P<0.001) and at 3 hours for triglycerides of 0.60 (0.43-0.78) mmol/L (P<0.001). Similarly, plasma proneurotensin and plasma triglycerides increased after ingestion of olive oil with maximum increase of proneurotensin at 3 hours of 62 (46-78) pmol/L (P<0.001) and plasma triglycerides at 3 hours of 0.32 (0.18-0.45) mmol/L (P<0.001). The post lipid load AUC for proneurotensin correlated significantly with the AUC for plasma triglycerides both after cream (r=0.49, P=0.021) and olive oil (r=0.55, P=0.008), respectively.Conclusion: Proneurotensin increases after an oral lipid load of both cream and olive oil and the degree of rise of post-ingestion proneurotensin is significantly related to the rise of post-ingestion plasma triglycerides. Our human data support recent results from animal studies suggesting that neurotensin contributes to intestinal absorption of lipids into the blood stream.

Is Fat Absorbed through the Peritoneum?

1985 ◽  
Vol 5 (3) ◽  
pp. 165-168 ◽  
Author(s):  
Ahmed Mitwalli Helen ◽  
Rodella Lidia Brandes ◽  
Ian Wanless Wu George ◽  
Raymond Ogilvie ◽  
Holger Schilling James ◽  
...  
Keyword(s):  
Lipid Droplets ◽  
Absolute Amount ◽  
Dilution Technique ◽  
Fat Emulsion ◽  
Initial Value ◽  
Histological Changes ◽  
Plasma Triglycerides ◽  
Transmission Electron ◽  
Dialysis Solution ◽  
Total Fat

A 10% fat solution (Liposyn) was infused (50 mi/kg) into the peritoneal cavity of four normal rabbits and left for five hours. Changes in the intraperitoneal volume were measured by using the radioactive albumin dilution technique. After an initial ultrafiltration, which peaked at one hour, the solution began to be absorbed and approximately 30% of the initial volume was removed at five hours. At the end of the infusion, dialysate fat concentration decreased to 85% of the initial value but the absolute amount absorbed was approximately 40% of the infused amount. Plasma total fat increased from 0.16 ± 0.03 to 1.35 ± 0.49 g/100 ml and plasma triglycerides increased from 94.7 ± 57.5 to 1247.8 ± 6.46 mg/100 ml. We observed no histological changes in the liver of the rabbits and the transmission electron microscopy demonstrated passage of lipid droplets into the mesenteric interstitium. The presence of droplets in micropinocytotic vesicles indicates that at least some of the lipid is transported through the cell by vesicular diacytosis. Our data indicate that fat emulsion is absorbed through the peritoneum slowly as the infused volume is absorbed. Under these conditions the solution cannot be used as a dialysis solution, but may be used for total parenteral nutrition in patients with normal kidney function who have no vascular access.

The Fine Structure of Rat Granulosa Cell Cultures Correlated with Progestin Secretion

1973 ◽  
Vol 31 ◽  
pp. 552-553
Author(s):  
T. M. Crisp ◽  
F.R. Denys
Keyword(s):  
Fine Structure ◽  
Granulosa Cell ◽  
Cell Cultures ◽  
Single Injection ◽  
Surrounding Medium ◽  
Petri Dish ◽  
Female Rats ◽  
Vaginal Smear ◽  
Immature Female ◽  
Serum Gonadotropin

The purpose of this paper is to present observations on the fine structure of rat granulosa cell cultures grown in the presence of an adenohypophyseal explant and to correlate the morphology of these cells with progestin secretion. Twenty-six day old immature female rats were given a single injection of 5 IU pregnant mares serum gonadotropin (PMS) in order to obtain ovaries with large vesicular follicles. At 66 hrs. post-PMS administration (estrus indicated by vaginal smear cytology), the ovaries were removed and placed in a petri dish containing medium 199 and 100 U penicillin/streptomycin (P/S)/ml. Under a 20X magnification dissecting microscope, some 5-8 vesicular follicles/ovary were punctured and the granulosa cells were expressed into the surrounding medium. The cells were transferred to centrifuge tubes and spun down at 1000 rpm for 5 mins.

Processing of human melanoma cells for correlative TEM, LVSEM, and LM

1991 ◽  
Vol 49 ◽  
pp. 106-107
Author(s):  
Marek Malecki ◽  
J. Victor Small ◽  
James Pawley
Keyword(s):  
Electron Microscopy ◽  
Low Voltage ◽  
Fluorescent Microscopy ◽  
Blood Stream ◽  
Surface Topology ◽  
Integrin Receptors ◽  
Transmission Electron ◽  
Develop Technology ◽  
Voltage Scanning ◽  
Mechanisms Of Adhesion

The relative roles of adhesion and locomotion in malignancy have yet to be clearly established. In a tumor, subpopulations of cells may be recognized according to their capacity to invade neighbouring tissue,or to enter the blood stream and metastasize. The mechanisms of adhesion and locomotion are themselves tightly linked to the cytoskeletal apparatus and cell surface topology, including expression of integrin receptors. In our studies on melanomas with Fluorescent Microscopy (FM) and Cell Sorter(FACS), we noticed that cells in cultures derived from metastases had more numerous actin bundles, then cells from primary foci. Following this track, we attempted to develop technology allowing to compare ultrastructure of these cells using correlative Transmission Electron Microscopy(TEM) and Low Voltage Scanning Electron Microscopy(LVSEM).

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