DISCUSSION PAPER: DIMETHYL SULFOXIDE AS A CRYOPROTECTIVE AGENT FOR PLATELET PRESERVATION BY FREEZING

The cryoprotective agents dimethyl sulfoxide (DMSO), glycerol, polyvinylpyrrolidone (PVP) and dextran were evaluated for their ability to protect avian cells during storage at sub-zero temperatures. DMSO was the most effective cryoprotective agent for the short- and long-term storage of avian cells and glycerol was also effective when used at low concentrations. PVP and dextran did not protect avian cells during storage in our experiments. Primary chicken cells and avian cells at higher passage levels were successfully recovered after storage with DMSO for periods ranging from 4 to 12 months.

Download Full-text

Influence de la température et de la durée d'un traitement cryoprotecteur sur la résistance au froid de plantules de blé. Étude ultrastructurale des nucléoles des ébauches foliaires

Canadian Journal of Botany ◽

10.1139/b85-084 ◽

1985 ◽

Vol 63 (4) ◽

pp. 663-671 ◽

Cited By ~ 4

Author(s):

Claude-Madeleine Gazeau

Keyword(s):

Dimethyl Sulfoxide ◽

Low Temperatures ◽

Leaf Primordia ◽

Distilled Water ◽

Wheat Seedlings ◽

Cryoprotective Agent

The nucleolus is sensitive to cold-protective media and to low temperatures. Nucleolar changes observed in the leaf primordia of wheat seedlings were related to the impregnation medium (distilled water or a mixture of glycerol, dimethyl sulfoxide, and sucrose), to the temperature (20 or 2 °C), and to the duration of the pretreatment (1 to 12 days). The cryoprotective agent generated the formation of nucleoli "with a diffuse granular zone." If the impregnation was done at 2 °C, characteristic nucleoli "with an indented aspect" occurred. The longer the duration of the impregnation and the more intense the subsequent freezing, the more important were the nucleolar changes. On rewarming the seedlings the various transformations were shown to be reversible.

Download Full-text

STORAGE OF MAMMALIAN OOCYTES AND EMBRYOS: A REVIEW

Canadian Journal of Animal Science ◽

10.4141/cjas76-018 ◽

1976 ◽

Vol 56 (2) ◽

pp. 131-145 ◽

Cited By ~ 11

Author(s):

RALPH R. MAURER

Keyword(s):

Dimethyl Sulfoxide ◽

Preimplantation Embryos ◽

Embryo Viability ◽

Cryoprotective Agent ◽

Delayed Implantation ◽

Rabbit Embryos

This article reviews the literature on storage of mammalian oocytes and embryos. Preimplantation embryos have been stored in vitro, in ligated oviducts and uteri of donor females, or in ligated oviducts of heterogenous females, and developed to term following transfer to suitable recipients. Embryo viability declined markedly after 48 h in storage anywhere other than in the reproductive tracts of species in which delayed implantation occurs naturally. Embryos stored at 0–37 C retained viability for 120 h; thereafter viability decreased. Storing oocytes in vitro at 10 C prolonged fertilizability for up to 3 days, but none developed to term if fertilized after 31 h storage. For successful storage, both cleavage and metabolism of embryos must be inhibited completely. This inhibition was accomplished with mouse and rabbit embryos by using dimethyl sulfoxide (DMSO) as the cryoprotective agent, slow rates of cooling (0.3–2 C/min) and warming (3–25 C/min), and a stepwise dilution of DMSO immediately after thawing. Neonates have resulted from the transfer of embryos previously frozen for 222 days.

Download Full-text

12. The ineffectiveness of dimethyl sulfoxide in preventing salt damage to frog sciatic nerves as opposed to its effectiveness as a cryoprotective agent

Cryobiology ◽

10.1016/s0011-2240(68)80017-3 ◽

1968 ◽

Vol 4 (5) ◽

pp. 251

Keyword(s):

Dimethyl Sulfoxide ◽

Cryoprotective Agent ◽

Salt Damage ◽

Sciatic Nerves

Download Full-text

Dextran Based Polyampholyte Having Cryoprotective Properties

MRS Proceedings ◽

10.1557/opl.2013.159 ◽

2013 ◽

Vol 1498 ◽

pp. 33-38

Author(s):

Minkle Jain ◽

Kazuaki Matsumura

Keyword(s):

Tissue Engineering ◽

Molecular Weight ◽

Dimethyl Sulfoxide ◽

High Molecular Weight ◽

Carboxyl Groups ◽

Engineering Applications ◽

L929 Cells ◽

Cryoprotective Agent

ABSTRACTDimethyl sulfoxide (DMSO) has been used for several decades as the most efficient cryoprotective agent (CPA) for many types of cells in spite of its cytotoxicity and its effect on differentiation. Recently we showed that carboxylated poly-L-lysine, which is classified as a polyampholyte, has a cryoprotective effect on cells in solution without any other cryoprotectant. Here we developed high molecular weight polyampholytes with an appropriate ratio of amino and carboxyl groups and evaluated their cryopreservation efficiency. A novel polyampholyte based on naturally available polymer dextran, in which we introduced both amino and carboxyl groups shows an excellent post thaw-survival efficiency of more than 90% of murine L929 cells. It can serve as the sole high molecular weight CPA for tissue engineering applications without animal derived materials.

Download Full-text

The Effects of Cryoprotective Agent on Spatiotemporal Deformation of Engineered Tissues During Freezing

ASME 2010 Summer Bioengineering Conference, Parts A and B ◽

10.1115/sbc2010-19277 ◽

2010 ◽

Author(s):

Ka Yaw Teo ◽

J. Craig Dutton ◽

Bumsoo Han

Keyword(s):

Dimethyl Sulfoxide ◽

Experimental Studies ◽

Optimum Composition ◽

Current Understanding ◽

Cryoprotective Agent ◽

Cryoprotective Agents ◽

Engineered Tissues

Maintaining tissue functionality is crucial for successful cryopreservation. However, current understanding of the effects of cryopreservation including those of cryoprotective agents (CPAs) on post-thaw tissue functionality is still very limited. In numerous cryopreservation protocols, CPAs such as dimethyl sulfoxide (DMSO) are commonly used. Different concentrations and combinations of CPAs have been reported to be advantageous in maintaining post-thaw tissue functionality, but the determination of their optimum composition often requires extensive experimental studies due to the lack of understanding of their effects on the biophysics of the tissues during freezing.

Download Full-text

Cryopreservation of Cyanobacteria and Eukaryotic Microalgae Using Exopolysaccharide Extracted from a Glacier Bacterium

Microorganisms ◽

10.3390/microorganisms9020395 ◽

2021 ◽

Vol 9 (2) ◽

pp. 395

Author(s):

Pervaiz Ali ◽

Daniel Fucich ◽

Aamer Ali Shah ◽

Fariha Hasan ◽

Feng Chen

Keyword(s):

Dimethyl Sulfoxide ◽

Hazardous Materials ◽

Cryoprotective Agent ◽

Growth Recovery ◽

Psychrotrophic Bacterium ◽

Poor Recovery ◽

Better Than

Exopolysaccharide (EPS) has been known to be a good cryoprotective agent for bacteria, but it has not been tested for cyanobacteria and eukaryotic microalgae. In this study, we used EPS extracted from a glacier bacterium as a cryoprotective agent for the cryopreservation of three unicellular cyanobacteria and two eukaryotic microalgae. Different concentrations of EPS (10%, 15%, and 20%) were tested, and the highest concentration (20%) of EPS yielded the best growth recovery for the algal strains we tested. We also compared EPS with 5% dimethyl sulfoxide (DMSO) and 10% glycerol for the cryopreservation recovery. The growth recovery for the microalgal strains after nine months of cryopreservation was better than 5% DMSO, a well-known cryoprotectant for microalgae. A poor recovery was recorded for all the tested strains with 10% glycerol as a cryoprotective agent. The patterns of growth recovery for most of these strains were similar after 5 days, 15 days, and 9 months of cryopreservation. Unlike common cryopreservants such as DMSO or methanol, which are hazardous materials, EPS is safe to handle. We demonstrate that the EPS from a psychrotrophic bacterium helped in the long-term cryopreservation of cyanobacteria and microalgae, and it has the potential to be used as natural cryoprotective agent for other cells.

Download Full-text