Dexamethasone Downregulates Mineralocorticoid Receptors in A6 Amphibian Kidney Cells in Tissue Culture

1995 ◽  
Vol 761 (1) ◽  
pp. 405-407
Author(s):  
RODNEY D. HAGLEY ◽  
CHARLES O. WATLINGTON
Keyword(s):  
Tissue Culture ◽  
Kidney Cells ◽  
Mineralocorticoid Receptors ◽  
Amphibian Kidney

A second rubella vaccine

1971 ◽  
Vol 9 (13) ◽  
pp. 52-52
Keyword(s):  
Tissue Culture ◽  
Pregnant Women ◽  
Rabbit Kidney ◽  
Kidney Cells ◽  
Animal Cells ◽  
Rubella Vaccine ◽  
Human Diploid Cells ◽  
Diploid Cells

Available vaccines used in protection against rubella employ strains of virus attenuated by passage in animal cells. The first vaccine that was introduced in Britain, the Cendehill vaccine (Cendevax),1 is grown on tissue culture of rabbit kidney cells. Recently another rubella vaccine (Almevax live attenuated, Wistar RA 27/3), grown on human diploid cells, has been introduced. This vaccine induces antibodies as effectively as earlier vaccines, and the indications for its use are similar to those for the Cendehill vaccine.1 2 It is very important that pregnant women, women who might be pregnant, or women likely to become pregnant within 2 months, must not be vaccinated.

AN ELECTRON MICROSCOPIC COMPARISON OF BOVINE INFECTIOUS PUSTULAR VULVOVAGINITIS VIRUS AND INFECTIOUS BOVINE RHINOTRACHEITIS VIRUS IN TISSUE CULTURE

1962 ◽  
Vol 8 (3) ◽  
pp. 373-378 ◽  
Author(s):  
I. Grinyer ◽  
M. Savan ◽  
M. J. Studdert
Keyword(s):  
Tissue Culture ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Phase Contrast ◽  
Kidney Cells ◽  
Infectious Bovine Rhinotracheitis ◽  
Electron Microscopic ◽  
Foetal Kidney ◽  
Electron Microscopes ◽  
Infectious Bovine Rhinotracheitis Virus

An electron microscopic study of IPV and IBR viruses isolated from cattle in Canada is presented. A technique is described with which it is possible to observe the cytopathic changes occurring in the same area of a monolayer, of bovine foetal kidney cells, infected with IPV or IBR virus, with both the phase contrast and electron microscopes. The morphology of these viruses was found to be identical and no difference was observed in their intracellular development.

IMPROVEMENTS IN THE TISSUE CULTURE SAFETY TESTING OF SALK VACCINES

1959 ◽  
Vol 5 (5) ◽  
pp. 453-459 ◽  
Author(s):  
R. C. French ◽  
R. E. Armstrong ◽  
W. Yarosh
Keyword(s):  
Tissue Culture ◽  
Volume Of Fluid ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Routine Test ◽  
Test Volume ◽  
Polio Virus ◽  
Safety Testing ◽  
First Order ◽  
Modified Test

Under the conditions of the official Canadian test, it was found that two simultaneous first-order reactions were in competition. Thus, after 4 hours, while 15% of any initial live MEF1 poliovirus present was adsorbed to the monkey kidney cells, 9% of the virus was destroyed by spontaneous inactivation. When the adsorption rate was increased by decreasing the volume of fluid, it was found that significantly more isolations of virus were obtained. Utilizing the method of Baron for the concentration of polio virus it was found possible to maintain a low test volume without making the routine test unduly cumbersome. The modified test is simpler and more sensitive and may be used with undialyzed vaccines.

DNA lesions induced in nuclei and nucleoli of monkey kidney cells in tissue culture by simian vacuolating virus (SV40)

1965 ◽  
Vol 66 (1) ◽  
pp. 33-48 ◽  
Author(s):  
Frederick H. Kasten ◽  
Colette Vendrely ◽  
Paul Tournier ◽  
Ren� Wicker
Keyword(s):  
Tissue Culture ◽  
Dna Lesions ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Virus Sv40

The antiviral activity of Isoprinosine

1972 ◽  
Vol 18 (9) ◽  
pp. 1463-1470 ◽  
Author(s):  
Paul Gordon ◽  
Eric R. Brown
Keyword(s):  
Tissue Culture ◽  
Antiviral Activity ◽  
Messenger Rna ◽  
Virus Titer ◽  
Kidney Cells ◽  
Newborn Mice ◽  
Polio Virus ◽  
Antiviral Effects ◽  
Rapid Labeling

A hypothesis predicting that increase in the rate of synthesis of host messenger RNA would be associated with antiviral activity was examined. Biochemically, Isoprinosine or NPT-10381 (methisoprinol) increased the rate of rapid labeling of polyribosomal RNA and protein in uninfected monkey kidney cells in tissue culture. In tissue-culture systems, Isoprinosine exerted antiviral effects against influenza virus, PR-8 and A2 strains; herpes virus, LU strain; polio virus 3; and adenovirus 10. In in vivo mortality studies, Isoprinosine was observed to exert therapeutic antiviral effects against the A2 strain of influenza and, as well, against a herpes infection of newborn mice. Experiments were carried out which demonstrated a correlation between the in vivo anti-influenza (PR-8) effects of Isoprinosine and reduction in the virus titer in lungs of infected mice.

scholarly journals In vitromethod for safety testing of foot-and-mouth disease vaccines

1970 ◽  
Vol 68 (2) ◽  
pp. 159-172 ◽  
Author(s):  
E. C. Anderson ◽  
P. B. Capstick ◽  
G. N. Mowat ◽  
F. B. Leech
Keyword(s):  
Tissue Culture ◽  
Culture System ◽  
Cell Sheet ◽  
Foot And Mouth Disease ◽  
Kidney Cells ◽  
Live Virus ◽  
Safety Testing ◽  
Safety Test ◽  
Antigen Present ◽  
Baby Hamster Kidney Cells

SummaryThe susceptibility of the tissue culture system to small amounts of residual live virus was not influenced by the inactivated antigen present. The depth of inoculum over the cell sheet did not affect results. Negative cultures frequently gave positive first (but not second or later) sub-cultures.Baby hamster kidney cells were always more sensitive than cattle tongues to infection with any of the strains used.Confidence in the safety test depends on the number of vaccination doses used; the tissue culture test can be made much more reliable than the cattle test because it is not limited to the 15 ml. of inoculum that restricts the cattle test.

The binding of the mucoprotein from gastric mucus to cells in tissue culture and the inhibition of cell adhesion

1975 ◽  
Vol 17 (3) ◽  
pp. 617-631
Author(s):  
A. Allen ◽  
S.M. Minnikin
Keyword(s):  
Tissue Culture ◽  
Cell Adhesion ◽  
Hela Cells ◽  
Polyoma Virus ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Gastric Mucus ◽  
Gastric Mucous ◽  
Baby Hamster Kidney Cells

The mucoprotein, which is responsible for the formation of gastric mucous gel in pig, has been shown to bind equally well to suspensions of baby hamster kidney cells, polyoma-virus-transformed baby hamster kidney cells and HeLa cells. The binding of the mucoprotein to the cells is dependent on Ca 2

scholarly journals Fibroblast receptor for cell-substratum adhesion: studies on the interaction of baby hamster kidney cells with latex beads coated by cold insoluble globulin (plasma fibronectin).

1980 ◽  
Vol 86 (1) ◽  
pp. 104-112 ◽  
Author(s):  
F Grinnell
Keyword(s):  
Tissue Culture ◽  
Divalent Cations ◽  
Cell Receptor ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Plasma Fibronectin ◽  
Bovine Albumin ◽  
Latex Beads ◽  
Cold Insoluble Globulin ◽  
Baby Hamster Kidney Cells

Studies were carried out on the interactions of uncharged latex beads (0.76 micrometer) with baby hamster kidney cells. Binding of beads to the cells occurred if the beads were coated by cold insoluble globulin (CIG) (plasma fibronectin) but not if the beads were coated by bovine albumin. Bovine albumin-coated beads did not bind to the cells even in the presence of excess CIG in the incubation medium. Binding of beads occurred randomly over the entire surfaces of cells in suspension. However, cell receptors for CIG beads were no longer detectable on the upper surface of cells spread onCIG-coated tissue culture dishes. Binding of CIG beads to cells occurred at all temperatures tested from 4 degrees to 37 degrees C but the rate was lowest at 4 degrees C. At 37 degrees C, binding was accompanied by endocytosis and the beads were found inside vesicles which appeared to be lysosomes. There was also release of radioactivity from radiolabeled CIG beads during incubation with the cells at 37 degrees C. Binding of CIG beads to cells did not require divalent cations. Finally, the cell receptor for CIG beads was lost after cell trypsinization. The data are discussed in terms of current ideas about the basis for cell adhesion.

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