An Advanced In Vitro Model to Study Hypoxia/Low Glucose-Induced Neuronal Cell Damage and Death

1997 ◽  
Vol 825 (1 Neuroprotecti) ◽  
pp. 267-278 ◽  
Author(s):  
M. P. TABOR ◽  
H. B. WORP ◽  
P. SODAAR ◽  
H. VELDMAN ◽  
E. A. J. JOOSTEN ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Cell Damage ◽  
Neuronal Cell ◽  
Low Glucose ◽  
Vitro Model

scholarly journals Estimation of the Genotoxicityof Hydrogen Peroxide and Antioxidant Actionof Halo Acid by the Method of Dna-Cometwith the Use of the Model of Transplantable Cell Cultures

2018 ◽  
pp. 40-43
Author(s):  
Olga Verle ◽  
Oleg Ostrovskiy ◽  
Valerian Verovskiy ◽  
Galina Dudchenko
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
In Vitro Model ◽  
Cell Damage ◽  
Protective Action ◽  
Genetic Material ◽  
Optimal Level ◽  
Pharmacological Agents ◽  
Vitro Model

In the framework of the study, the degree of defragmentation of DNA by the DNA-comet method is evaluated when exposed to the cell culture of hydrogen peroxide (H2O2), and an in vitro model is developed to evaluate the antioxidant activity of new pharmacological agents. The results of working with cell lines show that the percentage of damage to the genetic material of cells of intact samples does not greatly vary from the method of removing the cellular monolayer from the culture plastic. Concerning the effect of H2O2 as an inducer of oxidative stress on DNA cell damage, the optimal level of DNA defragmentation has been modeled for subsequent studies of the protective action of antioxidants.

Effects of Estrogen and Phytoestrogen Treatment on an In Vitro Model of Recurrent Stroke on HT22 Neuronal Cell Line

2016 ◽  
Vol 37 (3) ◽  
pp. 405-416 ◽  
Author(s):  
Javier Morán ◽  
Marcos Perez-Basterrechea ◽  
Pablo Garrido ◽  
Elena Díaz ◽  
Ana Alonso ◽  
...  
Keyword(s):  
Cell Line ◽  
In Vitro Model ◽  
Recurrent Stroke ◽  
Neuronal Cell ◽  
Neuronal Cell Line ◽  
Vitro Model

scholarly journals Compression Induced Cell Damage in Engineered Muscle Tissue: An In Vitro Model to Study Pressure Ulcer Aetiology

2003 ◽  
Vol 31 (11) ◽  
pp. 1357-1364 ◽  
Author(s):  
R. G. M. Breuls ◽  
C. V. C. Bouten ◽  
C. W. J. Oomens ◽  
D. L. Bader ◽  
F. P. T. Baaijens
Keyword(s):  
Muscle Tissue ◽  
Pressure Ulcer ◽  
In Vitro Model ◽  
Cell Damage ◽  
Vitro Model

Neuronal cell protective and antioxidant effects of phenolics obtained from Zanthoxylum piperitum leaf using in vitro model system

2011 ◽  
Vol 125 (2) ◽  
pp. 417-422 ◽  
Author(s):  
Chang-Ho Jeong ◽  
Ji Hyun Kwak ◽  
Ji Hye Kim ◽  
Gwi Nam Choi ◽  
Dae-Ok Kim ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Neuronal Cell ◽  
Model System ◽  
Zanthoxylum Piperitum ◽  
In Vitro Model System ◽  
Vitro Model ◽  
Antioxidant Effects

scholarly journals Pre-clinical evaluation of advanced nerve guide conduits using a novel 3D in vitro testing model

2018 ◽  
Vol 4 (1) ◽  
Author(s):  
Mehri Behbehani ◽  
Adam Glen ◽  
Caroline S. Taylor ◽  
Alexander Schuhmacher ◽  
Frederik Claeyssens ◽  
...  
Keyword(s):  
Schwann Cells ◽  
In Vitro Model ◽  
Ex Vivo ◽  
Donor Site ◽  
Neuronal Cell ◽  
Donor Site Morbidity ◽  
Animal Testing ◽  
Physical Support ◽  
Vitro Model

Autografts are the current gold standard for large peripheral nerve defects in clinics despite the frequently occurring side effects like donor site morbidity. Hollow nerve guidance conduits (NGC) are proposed alternatives to autografts, but fail to bridge gaps exceeding 3 cm in humans. Internal NGC guidance cues like microfibres are believed to enhance hollow NGCs by giving additional physical support for directed regeneration of Schwann cells and axons. In this study, we report a new 3D in vitro model that allows the evaluation of different intraluminal fibre scaffolds inside a complete NGC. The performance of electrospun polycaprolactone (PCL) microfibres inside 5 mm long polyethylene glycol (PEG) conduits was investigated in neuronal cell and dorsal root ganglion (DRG) cultures in vitro. Z-stack confocal microscopy revealed the aligned orientation of neuronal cells along the fibres throughout the whole NGC length and depth. The number of living cells in the centre of the scaffold was not significantly different to the tissue culture plastic (TCP) control. For ex vivo analysis, DRGs were placed on top of fibre-filled NGCs to simulate the proximal nerve stump. In 21 days of culture, Schwann cells and axons infiltrated the conduits along the microfibres with 2.2 ± 0.37 mm and 2.1 ± 0.33 mm respectively. We conclude that this in vitro model can help define internal NGC scaffolds in the future by comparing different fibre materials, composites and dimensions in one setup prior to animal testing.

scholarly journals Considerations for a Reliable In Vitro Model of Chemotherapy-Induced Peripheral Neuropathy

Toxics ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 300
Author(s):  
Sandy Eldridge ◽  
Arianna Scuteri ◽  
Eugenia M. C. Jones ◽  
Guido Cavaletti ◽  
Liang Guo ◽  
...  
Keyword(s):  
Cell Culture ◽  
Peripheral Neuropathy ◽  
Human Cell ◽  
Anticancer Agents ◽  
In Vitro Model ◽  
Neuronal Cell ◽  
Severe Toxicity ◽  
Human Cell Culture ◽  
Vitro Model

Chemotherapy-induced peripheral neuropathy (CIPN) is widely recognized as a potentially severe toxicity that often leads to dose reduction or discontinuation of cancer treatment. Symptoms may persist despite discontinuation of chemotherapy and quality of life can be severely compromised. The clinical symptoms of CIPN, and the cellular and molecular targets involved in CIPN, are just as diverse as the wide variety of anticancer agents that cause peripheral neurotoxicity. There is an urgent need for extensive molecular and functional investigations aimed at understanding the mechanisms of CIPN. Furthermore, a reliable human cell culture system that recapitulates the diversity of neuronal modalities found in vivo and the pathophysiological changes that underlie CIPN would serve to advance the understanding of the pathogenesis of CIPN. The demonstration of experimental reproducibility in a human peripheral neuronal cell system will increase confidence that such an in vitro model is clinically useful, ultimately resulting in deeper exploration for the prevention and treatment of CIPN. Herein, we review current in vitro models with a focus on key characteristics and attributes desirable for an ideal human cell culture model relevant for CIPN investigations.

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