scholarly journals Thermal Characterization In Vitro of Human Nail: Photoacoustic Study of the Aging Process

2007 ◽  
Vol 83 (5) ◽  
pp. 1144-1148 ◽  
Author(s):  
Daniele Toniolo Dias ◽  
Alysson Steimacher ◽  
Antonio Carlos Bento ◽  
Antonio Medina Neto ◽  
Mauro Luciano Baesso
Keyword(s):  
Aging Process ◽  
Thermal Characterization ◽  
Human Nail

scholarly journals Can Be miR-126-3p a Biomarker of Premature Aging? An Ex Vivo and In Vitro Study in Fabry Disease

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 356 ◽  
Author(s):  
Alessia Lo Curto ◽  
Simona Taverna ◽  
Maria Assunta Costa ◽  
Rosa Passantino ◽  
Giuseppa Augello ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Fabry Disease ◽  
Healthy Subjects ◽  
Aging Process ◽  
Replicative Senescence ◽  
Ex Vivo ◽  
Umbilical Vein ◽  
Quantitative Polymerase Chain Reaction ◽  
Premature Aging

Fabry disease (FD) is a lysosomal storage disorder (LSD) characterized by lysosomal accumulation of glycosphingolipids in a wide variety of cytotypes, including endothelial cells (ECs). FD patients experience a significantly reduced life expectancy compared to the general population; therefore, the association with a premature aging process would be plausible. To assess this hypothesis, miR-126-3p, a senescence-associated microRNA (SA-miRNAs), was considered as an aging biomarker. The levels of miR-126-3p contained in small extracellular vesicles (sEVs), with about 130 nm of diameter, were measured in FD patients and healthy subjects divided into age classes, in vitro, in human umbilical vein endothelial cells (HUVECs) “young” and undergoing replicative senescence, through a quantitative polymerase chain reaction (qPCR) approach. We confirmed that, in vivo, circulating miR-126 levels physiologically increase with age. In vitro, miR-126 augments in HUVECs underwent replicative senescence. We observed that FD patients are characterized by higher miR-126-3p levels in sEVs, compared to age-matched healthy subjects. We also explored, in vitro, the effect on ECs of glycosphingolipids that are typically accumulated in FD patients. We observed that FD storage substances induced in HUVECs premature senescence and increased of miR-126-3p levels. This study reinforces the hypothesis that FD may aggravate the normal aging process.

Anti-Aging Effect of Siraitia grosuenorii by Enhancement of Hematopoietic Stem Cell Function

2016 ◽  
Vol 44 (04) ◽  
pp. 803-815 ◽  
Author(s):  
Lin Bai ◽  
Guiying Shi ◽  
Yajun Yang ◽  
Wei Chen ◽  
Lianfeng Zhang
Keyword(s):  
Aging Process ◽  
Cell Function ◽  
Whooping Cough ◽  
Aging Effect ◽  
Guangxi Province ◽  
Facs Analysis ◽  
Hematopoietic Stem ◽  
Associated Proteins

Anti-aging has always been a popular topic, and there are many claims about the existence of factors that can slow, stop, or even reverse the aging process. Siraitia grosuenorii, a local fruit in china, has been used for the treatment of gastritis, sore throats, and whooping cough in traditional Chinese medicine. The individuals who took the juice of Siraitia grosuenorii regularly had increased longevity in the Guangxi Province, which is located in the Southern part of China. In this paper, we fed mice with Siraitia grosuenorii for 10 months to identify the role of Siraitia grosuenorii in anti-aging and to investigate its corresponding mechanism. The results showed that mice fed with Siraitia grosuenorii displayed a slower aging process. The extension of the aging process was due to the enhanced function of HSCs. FACS analysis showed that the number of LSKs, LT-HSCs, ST-HSCs and MPPs from Siraitia grosuenorii mice was decreased. In vitro, a clonigenic assay showed that LT-HSCs from Siraitia grosuenorii mice increased the ability of self-renewal. Moreover, Siraitia grosuenorii mice maintained the quiescence of LSKs, decreased the level of ROS and reduced the amount of senescence associated β-gal positive cells. Furthermore, Siraitia grosuenorii mice decreased the expression of senescence-associated proteins. Siraitia grosuenorii maintained quiescence, decreased senescence and enhanced the function of HSCs, slowing the aging process of mice.

scholarly journals A systematic review of the neurobiological aspects of memory in the aging process

2011 ◽  
Vol 5 (4) ◽  
pp. 310-321
Author(s):  
Eduardo Moreira de Oliveira ◽  
Priscilla Tiemi Kissaki ◽  
Tiago Nascimento Ordonez ◽  
Thaís Bento Lima-Silva
Keyword(s):  
Systematic Review ◽  
Aging Process ◽  
Human Subjects ◽  
Basic Research ◽  
Brain Regions ◽  
Morphological Alterations ◽  
Search Terms ◽  
Processing Information ◽  
Normal Human

Abstract A systematic review of the neuroanatomical literature was performed to determine the neuropharmacological aspects most relevant to the study of memory processes. Articles were retrieved using the search terms "biology of memory", "memory and aging", "memory impairment", "elderly and memory," and their equivalents in Portuguese. Of the studies surveyed, five studies dealt with epidemiological and demographic issues, 12 were clinical trials i.e. were based on testing and implementation of instruments in human subjects, 33 studies were basic research involving studies of mice, rats and non-human primates, and biochemical and in vitro trials and finally, 52 studies were literature reviews or book chapters which in our view, fell into this category. Conclusions: The work sought to highlight which neural networks are most involved in processing information, as well as their location within brain regions and the way in which neurotransmitters interact with each other for the formation of these memories. Moreover, it was shown how memory changes during the normal human aging process, both positively and negatively, by analyzing the morphological alterations that occur in the brain of aging individuals.

scholarly journals Generation of Vascularized Brain Organoids to Study Neurovascular Interactions

2022 ◽  
Author(s):  
Zhen-Ge Luo ◽  
Xin-Yao Sun ◽  
Xiang-Chun Ju ◽  
Yang Li ◽  
Peng-Ming Zeng ◽  
...  
Keyword(s):  
Brain Development ◽  
Neural Development ◽  
Immune Cells ◽  
Aging Process ◽  
Neural Progenitors ◽  
Brain Disorders ◽  
Specific Population ◽  
Neurovascular Interactions ◽  
The Brain

The recently developed brain organoids have been used to recapitulate the processes of brain development and related diseases. However, the lack of vasculatures, which regulate neurogenesis, brain disorders, and aging process, limits the utility of brain organoids. In this study, we induced vessel and brain organoids respectively, and then fused two types of organoids together to obtain vascularized brain organoids. The fused brain organoids were engrafted with robust vascular network-like structures, and exhibited increased number of neural progenitors, in line with the possibility that vessels regulate neural development. Fusion organoids also contained functional blood-brain-barrier (BBB)-like structures, as well as microglial cells, a specific population of immune cells in the brain. The incorporated microglia responded actively to immune stimuli to the fused brain organoids. Thus, the fusion organoids established in this study allow modeling interactions between the neuronal and non-neuronal components in vitro, in particular the vasculature and microglia niche.

Performance of orthodontic aligners in the aging process: systematic review and in vitro study

2021 ◽  
Vol 92 (3) ◽  
pp. 335-341
Author(s):  
Intissar El Idrissi ◽  
Khalil El Mabrouk ◽  
Fatima Zaoui ◽  
Hicham Benyahia
Keyword(s):  
Systematic Review ◽  
Aging Process ◽  
In Vitro Study ◽  
Vitro Study

In Vitro Penetration of a Novel Oxaborole Antifungal (AN2690) Into the Human Nail Plate

2007 ◽  
Vol 96 (10) ◽  
pp. 2622-2631 ◽  
Author(s):  
Xiaoying Hui ◽  
Stephen J. Baker ◽  
Ronald C. Wester ◽  
Sherry Barbadillo ◽  
Anne K. Cashmore ◽  
...  
Keyword(s):  
Nail Plate ◽  
Human Nail

scholarly journals Roles of Resveratrol in Improving the Quality of Postovulatory Aging Oocytes In Vitro

Cells ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 1132 ◽  
Author(s):  
Yan-Li Sun ◽  
Shou-Bin Tang ◽  
Wei Shen ◽  
Shen Yin ◽  
Qing-Yuan Sun
Keyword(s):  
Binding Sites ◽  
Aging Process ◽  
Oocyte Quality ◽  
Reproductive Technology ◽  
Early Embryo ◽  
Normal Morphology ◽  
Sperm Binding ◽  
Postovulatory Aging

After ovulation, mammalian oocytes will undergo a time-dependent process of aging if they are not fertilized. This postovulatory aging (POA) seriously affects the oocyte quality and then impairs the subsequent fertilization and early embryo development, which should be avoided especially in assisted reproductive technology (ART). Resveratrol is an antioxidant substance that can scavenge free radicals and is effective in improving ovary functions. Here, mouse oocytes were used to investigate the effects and mechanisms of resveratrol on POA oocytes in vitro. With 1.0 µM resveratrol treatment during aging process, the rates of fertilization and blastocyst in POA oocytes increased significantly compared with those in the POA group. Resveratrol can reduce the loss of sperm binding sites by stabilizing Juno. Resveratrol can maintain the normal morphology of spindle and mitochondrion distribution and alleviate the levels of ROS and early apoptosis. Additionally, resveratrol can reduce the changes of H3K9me2. Therefore, resveratrol can significantly improve the quality of POA oocytes in vitro to enhance the rates of fertilization and blastocyst, which may be very helpful during the ART process.

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