Individual elution of noradrenaline (together with adrenaline), dopamine, 5-hydroxytryptamine and histamine from a single, strong cation exchange column, by means of mineral acid-organic solvent mixtures

1970 ◽  
Vol 22 (8) ◽  
pp. 625-627 ◽  
Author(s):  
C. V. Atack ◽  
T. Magnusson
Keyword(s):  
Organic Solvent ◽  
Cation Exchange ◽  
Mineral Acid ◽  
Solvent Mixtures ◽  
Exchange Column ◽  
Strong Cation Exchange ◽  
Cation Exchange Column

scholarly journals Structural similarity of chymopapain forms as indicated by circular dichroism

1989 ◽  
Vol 257 (1) ◽  
pp. 183-186 ◽  
Author(s):  
S Solis-Mendiola ◽  
R Zubillaga-Luna ◽  
A Rojo-Dominguez ◽  
A Hernandez-Arana
Keyword(s):  
Liquid Chromatography ◽  
High Resolution ◽  
Cation Exchange ◽  
Proteolytic Activity ◽  
Structural Similarity ◽  
Tertiary Structures ◽  
Exchange Column ◽  
Cation Exchange Column ◽  
Modified Proteins ◽  
Circular Dichroïsm

Four chymopapain forms were isolated by high-resolution liquid chromatography on a cation-exchange column. The three major forms possess nearly identical secondary and tertiary structures, as judged from their c.d. spectra; these components showed similar proteolytic activity and Mr values close to that of papain. The fourth isolated component seems to be a mixture of modified proteins.

Hemoglobin A1C by Hplc with the Pharmacia Mono S HR 5/N Cation-Exchange Column: Influence of Sample Protein Load on Optimal Chromatographic Conditions

1992 ◽  
Vol 38 (8) ◽  
pp. 1488-1490 ◽  
Author(s):  
J C Philcox ◽  
M R Haywood ◽  
A M Rofe
Keyword(s):  
Cation Exchange ◽  
Flow Rate ◽  
Chromatographic Separation ◽  
Hemoglobin A1c ◽  
Analysis Time ◽  
Exchange Column ◽  
Cation Exchange Column ◽  
Protein Load

Abstract The Pharmacia Mono S HR 5/5 column has been optimized for hemoglobin A1c analysis by HPLC by using a much smaller column load, decreased buffer flow rate, and a steeper gradient than was used in previously described methods. Superior chromatographic separation, shorter analysis time, and a greatly extended column life have resulted.

Determination of haemoglobin A1c by liquid chromatography using a new cation-exchange column

2003 ◽  
Vol 791 (1-2) ◽  
pp. 73-83 ◽  
Author(s):  
Maria Beatriz de la Calle Guntiñas ◽  
René Wissiack ◽  
Guy Bordin ◽  
Adela Rosa Rodrı́guez
Keyword(s):  
Liquid Chromatography ◽  
Cation Exchange ◽  
Haemoglobin A1c ◽  
Exchange Column ◽  
Cation Exchange Column
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