scholarly journals Transcriptomic approach and membrane fatty acid analysis to study the response mechanisms of Escherichia coli to thyme essential oil, carvacrol, 2-(E)-hexanal and citral exposure

2018 ◽  
Vol 125 (5) ◽  
pp. 1308-1320 ◽  
Author(s):  
L. Siroli ◽  
G. Braschi ◽  
A. de Jong ◽  
J. Kok ◽  
F. Patrignani ◽  
...  
2021 ◽  
pp. 1-12
Author(s):  
Mina Volic ◽  
Verica Djordjevic ◽  
Maja Vukasinovic-Sekulic ◽  
Natasa Obradovic ◽  
Zorica Knezevic-Jugovic ◽  
...  

The aim of this study was to develop a stable hydrogel carrier system for thyme essential oil (TEO) that could protect its sensitive polyphenol compounds. The impact of wall material (soy protein and alginate) on encapsulation efficiency and thymol release in simulated gastrointestinal conditions, was investigated. The release of thymol was ~ 80 % and 20 % in simulated gastric and pancreatic solutions, respectively. Thyme essential oil plays an important role as an antimicrobial and antioxidant agent. Results indicated that encapsulated TEO inside the hydrogel matrix exhibited antioxidant activity demonstrated by CUPRAC and ABTS analysis, even after thermal treatment of the beads, indicating the metal chelate effect as dominant. In vitro antimicrobial activity of encapsulated TEO has been studied against several pathogenic microorganisms such as Escherichia coli, Staphylococcus aureus, Bacillus cereus and Candida albicans. Beads coded as Ca-A1.5/SP1.5 showed anti-Candida albicans activity, while modified bead formulations Ca-A1.5/SP1.5* and Ca-A1.5/SP0.25** showed bactericidal activity against Escherichia coli and Staphylococcus aureus.


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