Use of synthetic oligonucleotides for determination of HTLV‐1 proviral load by real‐time PCR: a helpful alternative approach in the clinical management

2020 ◽  
Vol 129 (3) ◽  
pp. 768-774
Author(s):  
L.M. Bandeira ◽  
M.A.M. Puga ◽  
V.S. Paula ◽  
L.H.F. Demarchi ◽  
G.G.C. Lichs ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Clinical Management ◽  
Proviral Load ◽  
Alternative Approach ◽  
Synthetic Oligonucleotides

scholarly journals Robust CT-prediction algorithm for RT-PCR

Filomat ◽  
2016 ◽  
Vol 30 (4) ◽  
pp. 1103-1110 ◽  
Author(s):  
Melih Gunay ◽  
Rajarajeswari Balasubramaniyan
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pcr Amplification ◽  
Prediction Algorithm ◽  
Rt Pcr ◽  
Ct Value ◽  
Alternative Approach ◽  
Definition Of ◽  
Multiple Pathogens

Introduction of fluorescence-based Real-Time PCR (RT-PCR) also called qPCR is increasingly used to detect multiple pathogens simultaneously and rapidly by gene expression analysis of PCR amplification data. Real-time PCR data are analyzed after setting an arbitrary threshold that must intersect the signal curve in its exponential phase. The point at which the curve crosses the threshold is called CT (Cycle Threshold). This simple and arbitrary value however is not an elegant definition of CT value sometimes leads to conclusions that are either false positive or negative. Therefore, the purpose of this paper is to present a stable and consistent alternative approach for the definition and determination of CT value that leads to near zero false positives and negatives.

Determination of the panel of reference genes for quantitative real-time PCR in fetal and adult rat intestines

2021 ◽  
Author(s):  
Xiaoqian Lu ◽  
Yi Liu ◽  
Dingmei Zhang ◽  
Kexin Liu ◽  
Qian Wang ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Quantitative Real Time Pcr ◽  
Adult Rat

Real-Time PCR Determination of Escherichia coli Genomic DNA Contamination in Plasmid Preparations

2002 ◽  
Vol 301 (1) ◽  
pp. 151-153 ◽  
Author(s):  
Adrián Vilalta ◽  
Vanessa Whitlow ◽  
Terrie Martin
Keyword(s):  
Escherichia Coli ◽  
Real Time ◽  
Real Time Pcr ◽  
Genomic Dna ◽  
Dna Contamination

Multiplex real-time PCR for the detection of insect DNA and determination of contents of Tenebrio molitor, Locusta migratoria and Achaeta domestica in food

2019 ◽  
Vol 245 (3) ◽  
pp. 559-567 ◽  
Author(s):  
René Köppel ◽  
Rafael Schum ◽  
Michael Habermacher ◽  
Cindy Sester ◽  
Lucia Eugeni Piller ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Locusta Migratoria ◽  
Tenebrio Molitor

scholarly journals Real-Time PCR as a Versatile Tool for Investigating the Susceptibility of Human Herpesvirus 6 to Antiviral Agents

2003 ◽  
Vol 47 (9) ◽  
pp. 3021-3024 ◽  
Author(s):  
Muriel Macé ◽  
Chaysavanh Manichanh ◽  
Pascale Bonnafous ◽  
Stéphanie Précigout ◽  
David Boutolleau ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Antiviral Agents ◽  
Antiviral Drug ◽  
Human Herpesvirus ◽  
Drug Susceptibility ◽  
Human Herpesvirus 6 ◽  
Versatile Tool ◽  
Herpesvirus 6

ABSTRACT A quantitative real-time PCR assay was developed for the determination of antiviral drug susceptibility and growth kinetics of human herpesvirus 6. The susceptibility and fitness of a sensitive strain, HST, and its ganciclovir-resistant derivative, GCVR1, were then characterized, leading us to conclude that the mutations of this latter virus did not alter its fitness significantly.

Determination of Helicobacter pylori cagA, vacA genotypes with real-time PCR melting curve analysis

2001 ◽  
Vol 95 (1-6) ◽  
pp. 369-377 ◽  
Author(s):  
Agnes Ruzsovics ◽  
Bela Molnar ◽  
Zsuzsa Unger ◽  
Zsolt Tulassay ◽  
Laszlo Pronai
Keyword(s):  
Helicobacter Pylori ◽  
Real Time ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis
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