Proviral Load Determination of HTLV-1 and HTLV-2 in Patients’ Peripheral Blood Mononuclear Cells by Real-Time PCR

2014 ◽  
pp. 315-323 ◽  
Author(s):  
Claudio Casoli ◽  
Elisabetta Pilotti ◽  
Umberto Bertazzoni
Keyword(s):  
Real Time ◽  
Peripheral Blood Mononuclear Cells ◽  
Real Time Pcr ◽  
Peripheral Blood ◽  
Proviral Load ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Validation of a quantitative real-time PCR assay for HTLV-1 proviral load in peripheral blood mononuclear cells

2013 ◽  
Vol 193 (2) ◽  
pp. 536-541 ◽  
Author(s):  
Carolina Rosadas ◽  
Mauro Jorge Cabral-Castro ◽  
Ana Carolina Paulo Vicente ◽  
José Mauro Peralta ◽  
Marzia Puccioni-Sohler
Keyword(s):  
Real Time ◽  
Peripheral Blood Mononuclear Cells ◽  
Real Time Pcr ◽  
Peripheral Blood ◽  
Proviral Load ◽  
Mononuclear Cells ◽  
Quantitative Real Time Pcr ◽  
Pcr Assay ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals Upregulated MicroRNA-155 Expression in Peripheral Blood Mononuclear Cells and Fibroblast-Like Synoviocytes in Rheumatoid Arthritis

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Li Long ◽  
Ping Yu ◽  
Yanying Liu ◽  
Shiyao Wang ◽  
Ru Li ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Real Time ◽  
Peripheral Blood Mononuclear Cells ◽  
Real Time Pcr ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Expression Profiles ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Fibroblast Like Synoviocytes

Objective. This study was to screen for the miRNAs differently expressed in peripheral blood mononuclear cells (PBMC) of RA, to further identify the expression of miR-155 in RA PBMC and fibroblast-like synoviocytes (FLS), and to evaluate the function of miR-155 in RA-FLS.Methods. Microarray was used to screen for differentially expressed miRNAs in RA PBMC. miR-155 expression in PBMC and FLS of RA were identified by real-time PCR. Enforced overexpression and downexpression of miR-155 were used to investigate the function of miR-155 in RA-FLS. Expression of IKBKE which was previously identified as the actual target of miR-155 was examined by Western blot and real-time PCR in RA-FLS.Results. miR-155 levels were increased in both PBMC and FLS of RA and could be induced by TNF-α. Upregulation of miR-155 decreased MMP-3 levels and suppressed proliferation and invasion of RA-FLS. Inverse relationship between the expressions of miR-155 and the MMPs production-related protein IKBKE was found.Conclusion. An inflammatory milieu may alter miRNA expression profiles in rheumatoid arthritis. miR-155 is upregulated in RA-FLS, and it may be a protective factor against the inflammatory effect in part by attenuating expression of IKBKE.

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