Gene repression using synthetic small regulatory RNA in Methylorubrum extorquens

2021 ◽  
Author(s):  
Li‐ping Zhu ◽  
Su‐zhen Song ◽  
Song Yang
Keyword(s):  
Gene Repression ◽  
Regulatory Rna ◽  
Small Regulatory Rna

scholarly journals The Small Regulatory RNA SyR1/PsrR1 Controls Photosynthetic Functions in Cyanobacteria

2014 ◽  
Vol 26 (9) ◽  
pp. 3661-3679 ◽  
Author(s):  
J. Georg ◽  
D. Dienst ◽  
N. Schurgers ◽  
T. Wallner ◽  
D. Kopp ◽  
...  
Keyword(s):  
Regulatory Rna ◽  
Small Regulatory Rna

scholarly journals LuxS-dependent AI-2 production is not involved in global regulation of natural product biosynthesis inPhotorhabdusandXenorhabdus

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3471 ◽  
Author(s):  
Antje K. Heinrich ◽  
Merle Hirschmann ◽  
Nick Neubacher ◽  
Helge B. Bode
Keyword(s):  
Regulatory System ◽  
Regulatory Rna ◽  
Insect Larvae ◽  
Phenotypic Differences ◽  
Global Regulators ◽  
Autoinducer 2 ◽  
Small Regulatory Rna ◽  
Mutant Strains ◽  
Different Strains

The Gram-negative bacteriaPhotorhabdusandXenorhabdusare known to produce a variety of different natural products (NP). These compounds play different roles since the bacteria live in symbiosis with nematodes and are pathogenic to insect larvae in the soil. Thus, a fine tuned regulatory system controlling NP biosynthesis is indispensable. Global regulators such as Hfq, Lrp, LeuO and HexA have been shown to influence NP production ofPhotorhabdusandXenorhabdus. Additionally, photopyrones as quorum sensing (QS) signals were demonstrated to be involved in the regulation of NP production inPhotorhabdus.In this study, we investigated the role of another possible QS signal, autoinducer-2 (AI-2), in regulation of NP production. The AI-2 synthase (LuxS) is widely distributed within the bacterial kingdom and has a dual role as a part of the activated methyl cycle pathway, as well as being responsible for AI-2 precursor production. We deletedluxSin three different entomopathogenic bacteria and compared NP levels in the mutant strains to the wild type (WT) but observed no difference to the WT strains. Furthermore, the absence of the small regulatory RNAmicA, which is encoded directly upstream ofluxS, did not influence NP levels. Phenotypic differences between theP. luminescens luxSdeletion mutant and an earlier describedluxSdeficient strain ofP. luminescenssuggested that two phenotypically different strains have evolved in different laboratories.

Optimization of phage λ promoter strength for synthetic small regulatory RNA-based metabolic engineering

2016 ◽  
Vol 21 (4) ◽  
pp. 483-490 ◽  
Author(s):  
Minhui Sung ◽  
Seung Min Yoo ◽  
Ren Jun ◽  
Jae Eun Lee ◽  
Sang Yup Lee ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Promoter Strength ◽  
Regulatory Rna ◽  
Small Regulatory Rna

scholarly journals The FasX Small Regulatory RNA Negatively Regulates the Expression of Two Fibronectin-Binding Proteins in Group A Streptococcus

2015 ◽  
Vol 197 (23) ◽  
pp. 3720-3730 ◽  
Author(s):  
Jessica L. Danger ◽  
Nishanth Makthal ◽  
Muthiah Kumaraswami ◽  
Paul Sumby
Keyword(s):  
Cell Surface ◽  
Virulence Factors ◽  
Binding Proteins ◽  
Mrna Translation ◽  
Regulatory Rna ◽  
Collagen Binding ◽  
Content Type ◽  
Small Regulatory Rna ◽  
Group A ◽  
Fibronectin Binding

ABSTRACTThe group AStreptococcus(GAS;Streptococcus pyogenes) causes more than 700 million human infections each year. The success of this pathogen can be traced in part to the extensive arsenal of virulence factors that are available for expression in temporally and spatially specific manners. To modify the expression of these virulence factors, GAS use both protein- and RNA-based regulators, with the best-characterized RNA-based regulator being the small regulatory RNA (sRNA) FasX. FasX is a 205-nucleotide sRNA that contributes to GAS virulence by enhancing the expression of the thrombolytic secreted virulence factor streptokinase and by repressing the expression of the collagen-binding cell surface pili. Here, we have expanded the FasX regulon, showing that this sRNA also negatively regulates the expression of the adhesion- and internalization-promoting, fibronectin-binding proteins PrtF1 and PrtF2. FasX posttranscriptionally regulates the expression of PrtF1/2 through a mechanism that involves base pairing to theprtF1andprtF2mRNAs within their 5′ untranslated regions, overlapping the mRNA ribosome-binding sites. Thus, duplex formation between FasX and theprtF1andprtF2mRNAs blocks ribosome access, leading to an inhibition of mRNA translation. Given that FasX positively regulates the expression of the spreading factor streptokinase and negatively regulates the expression of the collagen-binding pili and of the fibronectin-binding PrtF1/2, our data are consistent with FasX functioning as a molecular switch that governs the transition of GAS between the colonization and dissemination stages of infection.IMPORTANCEMore than half a million deaths each year are a consequence of infections caused by GAS. Insights into how this pathogen regulates the production of proteins during infection may facilitate the development of novel therapeutic or preventative regimens aimed at inhibiting this activity. Here, we have expanded insight into the regulatory activity of the GAS small RNA FasX. In addition to identifying that FasX reduces the abundance of the cell surface-located fibronectin-binding proteins PrtF1/2, fibronectin is present in high abundance in human tissues, and we have determined the mechanism behind this regulation. Importantly, as FasX is the only mechanistically characterized regulatory RNA in GAS, it serves as a model RNA in this and related pathogens.

In silico ‘fishing’ using known small regulatory RNA (sRNA) candidates as the decoy from Escherichia coli, Salmonella typhi and Salmonella typhimurium manifested 14 novel sRNA candidates in the orthologous region of Proteus mirabilis

2018 ◽  
Vol 45 (6) ◽  
pp. 2333-2343 ◽  
Author(s):  
Selvaraju KishanRaj ◽  
Samuggam Sumitha ◽  
Balakrishnan Siventhiran ◽  
Othayakumar Thiviyaa ◽  
Kathiresan V. Sathasivam ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhimurium ◽  
Proteus Mirabilis ◽  
In Silico ◽  
Salmonella Typhi ◽  
Regulatory Rna ◽  
Orthologous Region ◽  
Small Regulatory Rna

scholarly journals Sustained translational repression of lactate dehydrogenase 1 inToxoplasma gondiibradyzoites is conferred by a small regulatory RNA hairpin

FEBS Journal ◽  
2014 ◽  
Vol 281 (22) ◽  
pp. 5077-5091 ◽  
Author(s):  
Michael Holmes ◽  
Vaunell Itaas ◽  
Sirinart Ananvoranich
Keyword(s):  
Lactate Dehydrogenase ◽  
Translational Repression ◽  
Regulatory Rna ◽  
Small Regulatory Rna ◽  
Rna Hairpin

scholarly journals Small Regulatory RNA and Legionella pneumophila

2011 ◽  
Vol 2 ◽  
Author(s):  
Sébastien P. Faucher ◽  
Howard A. Shuman
Keyword(s):  
Legionella Pneumophila ◽  
Regulatory Rna ◽  
Small Regulatory Rna

scholarly journals The small regulatory RNA molecule MicA is involved in Salmonella enterica serovar Typhimurium biofilm formation

2010 ◽  
Vol 10 (1) ◽  
pp. 276 ◽  
Author(s):  
Gwendoline Kint ◽  
David De Coster ◽  
Kathleen Marchal ◽  
Jos Vanderleyden ◽  
Sigrid CJ De Keersmaecker
Keyword(s):  
Salmonella Enterica ◽  
Biofilm Formation ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Regulatory Rna ◽  
Small Regulatory Rna ◽  
Serovar Typhimurium
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