A multi‐epitope chimeric protein elicited a strong antibody response and partial protection against Edwardsiella ictaluri in Nile tilapia

2021 ◽  
Author(s):  
Vimbai Irene Machimbirike ◽  
Natapol Pornputtapong ◽  
Saengchan Senapin ◽  
Eakapol Wangkahart ◽  
Prapansak Srisapoome ◽  
...  
Keyword(s):  
Antibody Response ◽  
Nile Tilapia ◽  
Chimeric Protein ◽  
Edwardsiella Ictaluri ◽  
Partial Protection ◽  
Strong Antibody Response

scholarly journals Active immunization with tau epitope in a mouse model of tauopathy induced strong antibody response together with improvement in short memory and pSer396-tau pathology

2020 ◽  
Vol 134 ◽  
pp. 104636 ◽  
Author(s):  
A. Joly-Amado ◽  
H. Davtyan ◽  
K. Serraneau ◽  
P. Jules ◽  
A. Zitnyar ◽  
...  
Keyword(s):  
Mouse Model ◽  
Antibody Response ◽  
Active Immunization ◽  
Tau Pathology ◽  
Strong Antibody Response ◽  
Short Memory

scholarly journals Naturally acquired antibody response to a Plasmodium falciparum chimeric vaccine candidate GMZ2.6c and its components (MSP-3, GLURP, and Pfs48/45) in individuals living in Brazilian malaria-endemic areas

2022 ◽  
Vol 21 (1) ◽  
Author(s):  
Barbara Oliveira Baptista ◽  
Ana Beatriz Lopes de Souza ◽  
Evelyn Kety Pratt Riccio ◽  
Cesare Bianco-Junior ◽  
Paulo Renato Rivas Totino ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Antibody Response ◽  
Vaccine Candidate ◽  
Chimeric Protein ◽  
The State ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Multi Stage ◽  
Endemic Areas ◽  
Different Levels

Abstract Background The GMZ2.6c malaria vaccine candidate is a multi-stage Plasmodium falciparum chimeric protein which contains a fragment of the sexual-stage Pfs48/45-6C protein genetically fused to GMZ2, a fusion protein of GLURP and MSP-3, that has been shown to be well tolerated, safe and immunogenic in clinical trials performed in a malaria-endemic area of Africa. However, there is no data available on the antigenicity or immunogenicity of GMZ2.6c in humans. Considering that circulating parasites can be genetically distinct in different malaria-endemic areas and that host genetic factors can influence the immune response to vaccine antigens, it is important to verify the antigenicity, immunogenicity and the possibility of associated protection in individuals living in malaria-endemic areas with different epidemiological scenarios. Herein, the profile of antibody response against GMZ2.6c and its components (MSP-3, GLURP and Pfs48/45) in residents of the Brazilian Amazon naturally exposed to malaria, in areas with different levels of transmission, was evaluated. Methods This study was performed using serum samples from 352 individuals from Cruzeiro do Sul and Mâncio Lima, in the state of Acre, and Guajará, in the state of Amazonas. Specific IgG, IgM, IgA and IgE antibodies and IgG subclasses were detected by Enzyme-Linked Immunosorbent Assay. Results The results showed that GMZ2.6c protein was widely recognized by naturally acquired antibodies from individuals of the Brazilian endemic areas with different levels of transmission. The higher prevalence of individuals with antibodies against GMZ2.6c when compared to its individual components may suggest an additive effect of GLURP, MSP-3, and Pfs48/45 when inserted in a same construct. Furthermore, naturally malaria-exposed individuals predominantly had IgG1 and IgG3 cytophilic anti-GMZ2.6c antibodies, an important fact considering that the acquisition of anti-malaria protective immunity results from a delicate balance between cytophilic/non-cytophilic antibodies. Interestingly, anti-GMZ2.6c antibodies seem to increase with exposure to malaria infection and may contribute to parasite immunity. Conclusions The data showed that GMZ2.6c protein is widely recognized by naturally acquired antibodies from individuals living in malaria-endemic areas in Brazil and that these may contribute to parasite immunity. These data highlight the importance of GMZ2.6c as a candidate for an anti-malarial vaccine.

Immunoproteomic analysis of the antibody response obtained in Nile tilapia following vaccination with a Streptococcus iniae vaccine

2011 ◽  
Vol 152 (3-4) ◽  
pp. 346-352 ◽  
Author(s):  
Benjamin R. LaFrentz ◽  
Craig A. Shoemaker ◽  
Phillip H. Klesius
Keyword(s):  
Antibody Response ◽  
Nile Tilapia ◽  
Streptococcus Iniae

scholarly journals Interplay of diverse adjuvants and nanoparticle presentation of native-like HIV-1 envelope trimers

npj Vaccines ◽  
2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Kwinten Sliepen ◽  
Edith Schermer ◽  
Ilja Bontjer ◽  
Judith A. Burger ◽  
Réka Felfödiné Lévai ◽  
...  
Keyword(s):  
Antibody Response ◽  
Rank Order ◽  
Neutralizing Antibody ◽  
Strong Antibody Response ◽  
Target Effect ◽  
Hiv 1

AbstractThe immunogenicity of HIV-1 envelope (Env) trimers is generally poor. We used the clinically relevant ConM SOSIP trimer to compare the ability of different adjuvants (squalene emulsion, ISCOMATRIX, GLA-LSQ, and MPLA liposomes) to support neutralizing antibody (NAb) responses in rabbits. The trimers were administered as free proteins or on nanoparticles. The rank order for the adjuvants was ISCOMATRIX > SE > GLA-LSQ ~ MPLA liposomes > no adjuvant. Stronger NAb responses were elicited when the ConM SOSIP trimers were presented on ferritin nanoparticles. We also found that the GLA-LSQ adjuvant induced an unexpectedly strong antibody response to the ferritin core of the nanoparticles. This “off-target” effect may have compromised its ability to induce the more desired antitrimer antibodies. In summary, both adjuvants and nanoparticle display can improve the magnitude of the antibody response to SOSIP trimers but the best combination of trimer presentation and adjuvant can only be identified experimentally.

scholarly journals Dynamically evolving novel overlapping gene as a factor in the SARS-CoV-2 pandemic

2020 ◽  
Author(s):  
Chase W. Nelson ◽  
Zachary Ardern ◽  
Tony L. Goldberg ◽  
Chen Meng ◽  
Chen-Hao Kuo ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Antibody Response ◽  
Protein Sequence ◽  
Evolutionary Analysis ◽  
Overlapping Genes ◽  
Emerging Viruses ◽  
Consensus Sequences ◽  
Overlapping Gene ◽  
Strong Antibody Response ◽  
Accessory Genes

AbstractUnderstanding the emergence of novel viruses requires an accurate and comprehensive annotation of their genomes. Overlapping genes (OLGs) are common in viruses and have been associated with pandemics, but are still widely overlooked. We identify and characterize ORF3d, a novel OLG in SARS-CoV-2 that is also present in Guangxi pangolin-CoVs but not other closely related pangolin-CoVs or bat-CoVs. We then document evidence of ORF3d translation, characterize its protein sequence, and conduct an evolutionary analysis at three levels: between taxa (21 members of Severe acute respiratory syndrome-related coronavirus), between human hosts (3978 SARS-CoV-2 consensus sequences), and within human hosts (401 deeply sequenced SARS-CoV-2 samples). ORF3d has been independently identified and shown to elicit a strong antibody response in COVID-19 patients. However, it has been misclassified as the unrelated gene ORF3b, leading to confusion. Our results liken ORF3d to other accessory genes in emerging viruses and highlight the importance of OLGs.

scholarly journals Ontogeny of Nile tilapia (Oreochromis niloticus) Immunoglobulin Type M Antibody Response

2015 ◽  
Author(s):  
Sakda Daduang ◽  
Piti Ungarreevittaya ◽  
Jureerut Daduang ◽  
Kittipoj Ngamcharoen ◽  
Bundit Tengjaroenkul ◽  
...  
Keyword(s):  
Antibody Response ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Nile Tilapia Oreochromis Niloticus

scholarly journals Strong antibody response after a first dose of a SARS‐CoV‐2 mRNA‐based vaccine in kidney transplant recipients with a previous history of COVID‐19

2021 ◽  
Author(s):  
Ilies Benotmane ◽  
Gabriela Gautier ‐Vargas ◽  
Floriane Gallais ◽  
Pierre Gantner ◽  
Noelle Cognard ◽  
...  
Keyword(s):  
Antibody Response ◽  
Kidney Transplant ◽  
Previous History ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients ◽  
Strong Antibody Response ◽  
History Of

A peptide vaccine based on a B-cell epitope on the VP1 protein of enterovirus 70 induces a strong antibody response

2012 ◽  
Vol 56 (04) ◽  
pp. 337-342 ◽  
Author(s):  
KEE-BUM PARK ◽  
BYUNG-KWAN LIM ◽  
MICHAEL B. YE ◽  
SOO-YOUNG CHUNG ◽  
JAE-HWAN NAM
Keyword(s):  
Antibody Response ◽  
B Cell ◽  
Peptide Vaccine ◽  
Cell Epitope ◽  
Vp1 Protein ◽  
Strong Antibody Response ◽  
B Cell Epitope ◽  
Enterovirus 70

scholarly journals Short communication: a modified Vaccinia virus Ankara-based Porcine circovirus 2 vaccine elicits strong antibody response upon prime-boost homologous immunization in a preclinical model

2020 ◽  
Vol 51 (3) ◽  
pp. 1439-1445
Author(s):  
Danielle Soares de Oliveira Daian e Silva ◽  
Edel Figueiredo Barbosa-Stancioli ◽  
Jordana Graziela Alves Coelho-Dos-Reis ◽  
Flávio Guimarães Da Fonseca
Keyword(s):  
Antibody Response ◽  
Vaccinia Virus ◽  
Short Communication ◽  
Porcine Circovirus ◽  
Preclinical Model ◽  
Strong Antibody Response ◽  
Modified Vaccinia Virus Ankara ◽  
Porcine Circovirus 2
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