Development of nutritious rice with high zinc/selenium and low cadmium in grains through QTL pyramiding

Abstract Background Multicentric Castleman’s disease is a life-threatening disorder involving a systemic inflammatory response and multiple organ failure caused by the overproduction of interleukin-6. Although renal complications of Castleman’s disease include AA amyloidosis, thrombotic microangiopathy, and membranoproliferative glomerulonephritis, membranous nephropathy is relatively rare. We experienced a case of secondary membranous nephropathy associated with Castleman’s disease. Case presentation The patient was a 43-year-old Japanese man who had shown a high zinc sulfate value in turbidity test, polyclonal hypergammaglobulinemia, anemia, and proteinuria. A physical examination revealed diffuse lymphadenopathy, an enlarged spleen and papulae of the body trunk. A skin biopsy of a papule on the patient’s back showed plasma cells in the perivascular area and he was diagnosed with multicentric Castleman’s disease, plasma cell variant. Kidney biopsy showed the appearance of bubbling in the glomerular basement membranes in Periodic acid methenamine silver stain and electron microscopy revealed electron dense deposits within and outside the glomerular basement membranes. Since immunofluorescence study showed predominant granular deposition of IgG1 and IgG2, he was diagnosed with secondary membranous nephropathy associated with Castleman’s disease. He was initially treated with prednisolone alone, however his biochemical abnormalities did not improve. After intravenous tocilizumab (700 mg every 2 weeks) was started, his C-reactive protein elevation, anemia, and polyclonal gammopathy improved. Furthermore, his urinary protein level declined from 1.58 g/gCr to 0.13 g/gCr. The prednisolone dose was gradually tapered, then discontinued. He has been stable without a recurrence of proteinuria for more than 6 months. Conclusions Tocilizumab might be a treatment option for secondary membranous nephropathy associated with Castleman’s disease.

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Effect of Zinc Supplementation on Innate and Adaptive Immunity in Lao Children at Risk of Zinc Deficiency (P19-013-19)

Current Developments in Nutrition ◽

10.1093/cdn/nzz049.p19-013-19 ◽

2019 ◽

Vol 3 (Supplement_1) ◽

Author(s):

Gertrud Schuster ◽

Chidchamai Kewcharoenwong ◽

Maxwell Barffour ◽

Guy-Marino Hinnouho ◽

Janet Peerson ◽

...

Keyword(s):

T Cell ◽

Venous Blood ◽

Analysis Of Covariance ◽

T Cell Subsets ◽

Secondary Outcome ◽

Plasma Zinc ◽

Blood Leukocytes ◽

Double Blind ◽

Significant Group ◽

High Zinc

Abstract Objectives Determine if children (6 to 23 mo) who received daily preventive zinc (PZ; 7 mg/d), daily high-zinc, low-iron micronutrient powder (MNP; 10 mg/d zinc, 6 mg/d iron) or therapeutic zinc during episodes of diarrhea (TZ; 20 mg/d for 10 d per episode) have improved markers of innate and adaptive immune function, compared to placebo (PL). Methods Rural Laotian children were recruited into a double-blind, randomized, controlled intervention trial for 9 mo. Venous blood was collected at baseline (BL) and endline (EL) for analysis. Primary outcomes included T-cell subsets (naïve and memory CD4, CD8, Tregs) measured by flow cytometry and production of T-cell cytokines (IL-2, IL-10, IL-13, IL-17A, INF-γ) and LPS-induced cytokines (IL-1β, IL-6, IL-10, TNF-α by whole blood cultures. Blood leukocytes (including lymphocytes, neutrophils, monocytes and eosinophils) were measured as secondary outcome variables. Group means at EL were compared by analysis of covariance (controlling for BL values of the outcome, sex, child age, district, month of enrollment and plasma zinc concentration). If an interaction with BL plasma zinc (above/below median) was observed, group means were compared separately in children above and below the median. Results Mean BL plasma zinc in all children (N = 574) was 0.55 ± 0.12 mg/L. No significant group differences were seen at EL in the primary outcomes. For secondary outcomes, the counts (^103/μL) of lymphocytes from the PZ group (5.02 ± 0.16) were significantly lower than the PL group (5.64 ± 0.16; P = 0.032). The EL counts (^103/μL) of from the PZ group (0.144 ± 0.026) were significantly lower than in the PL (0.279 ± 0.048; P = 0.036) and TZ (0.285 ± 0.047; P = 0.025) groups among children with baseline zinc below the median. Conclusions Primary outcomes (T-cell subsets, and cytokine production) were not affected by the zinc intervention. Lymphocyte and eosinophil concentrations may be affected by zinc treatment but this result requires confirmation. Funding Sources ARS Project 2032-53000-001-00-D, Mathile Institute for the Advancement of Human Nutrition.

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Tolerance to heavy metals in Littorina saxatilis from a metal contaminated estuary in the Isle of Man

Journal of the Marine Biological Association of the United Kingdom ◽

10.1017/s0025315404009336h ◽

2004 ◽

Vol 84 (2) ◽

pp. 393-400 ◽

Cited By ~ 12

Author(s):

Erema R. Daka ◽

Stephen J. Hawkins

Keyword(s):

Littorina Saxatilis ◽

Co Tolerance ◽

Isle Of Man ◽

Run Off ◽

High Zinc ◽

Lead Tolerance ◽

Test Concentration ◽

A Site ◽

High Lead ◽

Tolerance To Copper

Population differences were measured in the tolerance of Littorina saxatilis from sites around the Isle of Man, to acute exposure to zinc, lead, copper and cadmium. Animals from a site influenced by disused mine run-off in Laxey estuary (high zinc) were compared with animals from less contaminated estuaries (Peel-high lead, but lower zinc), and the relatively uncontaminated Castletown and Ramsey estuaries, plus the open coast near Derbyhaven. Median lethal times (LT50) were estimated for each test concentration (5, 10, 20 mg l−1 Zn; 5, 10 mg l−1 Pb; 0·5, 1·0, 2·0 mg l−1 Cu and Cd) except for those that did not produce sufficient mortalities. Individuals from Laxey estuary showed significantly higher tolerances to zinc (10 mg l−1) and lead (5 mg l−1) than animals from the unpolluted sites. No co-tolerance to copper or cadmium was apparent. Population tolerance to zinc was correlated with reduced accumulation rates. Lead tolerance may result from the ability of the tolerant individuals to sequester the metal and detoxify it in their tissues; the littorinids from Laxey had significantly higher rates of lead accumulation.

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Subacute Toxicity of Cyclopiazonic Acid in Broiler Chicks Fed Normal and High Zinc Diets

Biodeterioration Research ◽

10.1007/978-1-4757-9453-3_11 ◽

1990 ◽

pp. 151-160 ◽

Cited By ~ 5

Author(s):

Mark E. Wilson ◽

Winston M. Hagler ◽

Jon F. Ort ◽

John M. Cullen ◽

Richard J. Cole

Keyword(s):

Cyclopiazonic Acid ◽

Broiler Chicks ◽

Subacute Toxicity ◽

High Zinc

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High-zinc rice as a breakthrough for high nutritional rice breeding program

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/129/1/012004 ◽

2018 ◽

Vol 129 ◽

pp. 012004

Author(s):

U Barokah ◽

U Susanto ◽

M Swamy ◽

D W Djoar ◽

Parjanto

Keyword(s):

Breeding Program ◽

Rice Breeding ◽

High Zinc

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Evaluation of Recombinant Inbred Lines for Higher Iron and Zinc Content Along with Yield and Quality Parameters in Rice (Oryza sativa L.)

Indian Journal of Agricultural Research ◽

10.18805/ijare.a-5454 ◽

2020 ◽

Author(s):

Maini Bhattacharjee ◽

Kasturi Majumder ◽

Sabyasachi Kundagrami ◽

Tapash Dasgupta

Keyword(s):

Iron Content ◽

Oryza Sativa L ◽

Recombinant Inbred Lines ◽

Zinc Content ◽

Correlation Study ◽

Inbred Lines ◽

Quality Parameters ◽

Fe Content ◽

High Zinc ◽

Iron And Zinc

Rice is one of the most important staple food crops for billions of people throughout the world. It is the cheapest source of dietary energy, protein and minerals for people but poor in micronutrients such as Fe and Zn to eliminate “hidden hunger”. In the present study, a population of 126recombinant inbred lines developed from a cross between Lemont X Satabdi (IET 4786) were used to identify high iron and zinc content coupled with yield and yield attributing traits. Analysis of Variance revealed that a considerable variation in iron and zinc existed among genotypes. The correlation study revealed that number of filled grain was positively associated with panicle length and yield per plant and number of panicles, but no significant positive correlation was observed between grain zinc content and iron content. Zinc and iron content of rice was estimated using Atomic Absorption Spectrophotometer and the samples were prepared by tri-acid digestion method. Among RIL lines, the line 57, 97,120, 48, 99, 124 contained more than 30 ppm Fe and the lines 24, 6, 9, 23, 29, 125 were found to possess more than 50 ppm Zn. The lines 9,6,48 and 57 were recorded to be high yielding with high zinc and Fe content in grain and in future these four lines look promising for multi location trial also. These high Fe and Zn content genotypes can be utilized in future breeding programme as a donor or good source for bio fortification of rice genotypes.

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Mycobacterial resistance to zinc poisoning requires assembly of P-ATPase-containing membrane metal efflux platforms

10.1101/2021.10.01.462712 ◽

2021 ◽

Author(s):

Yves-Marie Boudehen ◽

Marion Faucher ◽

Xavier Marechal ◽

Roger Miras ◽

Jerome Rech ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Metal Binding ◽

Bacterial Resistance ◽

Binding Motif ◽

Membrane Microdomain ◽

High Zinc ◽

Functional Membrane ◽

Zinc Concentrations ◽

Metal Efflux ◽

High Concentrations

Transition metals are toxic at high concentrations. The P1B-ATPase metal exporter CtpC/Rv3270 is required for resistance to zinc poisoning in the human pathogen Mycobacterium tuberculosis. Here, we discovered that zinc resistance also depends on the chaperone-like protein PacL1/Rv3269. PacL1 bound Zn2+, but unlike PacL1 and CtpC, the PacL1 metal-binding motif (MBM) was required only at high zinc concentrations. PacL1 co-localized with CtpC in dynamic microdomains within the mycobacterial plasma membrane. Microdomain formation did not require flotillins nor the PacL1 MBM. Instead, loss of the PacL1 Glutamine/Alanine repeats led to loss of CtpC and sensitivity to zinc. PacL1 and CtpC are within the same operon, and homologous PacL1-P1B-ATPase pairs are widely distributed within and across prokaryotes. PacL1 colocalized and functioned redundantly with PacL orthologs in Mycobacterium tuberculosis. Overall, our study suggests that PacL proteins are scaffolds that assemble P-ATPase-containing metal efflux platforms, a novel type of functional membrane microdomain that underlies bacterial resistance to metal poisoning.

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Two-stage SART process: A feasible alternative for gold cyanidation plants with high zinc and copper contents

10.3390/iecms2018-05449 ◽

2018 ◽

Author(s):

Humberto Estay ◽

Minghai Gim-Krumm ◽

Michelle Quilaqueo

Keyword(s):

Two Stage ◽

High Zinc ◽

Feasible Alternative

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The role of zinc status in altered cardiac adenylate cyclase activity in diabetic rats

Acta Endocrinologica ◽

10.1530/acta.0.1190174 ◽

1988 ◽

Vol 119 (2) ◽

pp. 174-180 ◽

Cited By ~ 10

Author(s):

Arshag D. Mooradian ◽

John E. Morley ◽

Philip J. Scarpace

Keyword(s):

Adenylate Cyclase ◽

Adrenergic Receptor ◽

Diabetic Rats ◽

Cyclase Activity ◽

Adenylate Cyclase Activity ◽

Zinc Status ◽

Dietary Zinc ◽

Zinc Intake ◽

Diabetic State ◽

High Zinc

Abstract. Zinc deficiency and altered myocardial adenylate cyclase activity commonly occur in diabetes. To determine whether the zinc intake of the animal can account for the altered β-adrenergic receptor activity in the diabetic heart, we determined the β-adrenergic receptor number and isoproterenol-, NaF- and forskolin-stimulated adenylate cyclase activity in diabetic and control rats maintained on low, normal and high zinc diets for 3 weeks. Scatchard analysis of [125I]iodocyanopindolol binding to control heart membrane preparations revealed a binding capacity of 17.3 ± 1.3 fmol/mg protein with a Kd of 35 ± 1.0 pmol/l. Neither the diabetic state nor the zinc status altered these binding parameters. The isoproterenol-stimulated adenylate cyclase acticity was significantly lower in diabetic rats on low zinc diets compared with controls. The NaF- (65.1 ± 5.4 vs 60.8 ± 6.4 pmol cAMP·mg protein−1·min−1) and forskolin-stimulated adenylate cyclase activities (161 ± 9.3 vs 154 ± 21.2 pmol cAMP·mg protein−1· min−1) were not significantly altered in diabetic rats. Low dietary zinc intake compared with high zinc diet significantly increased NaF- and forskolin-stimulated adenylate cyclase activity both in diabetic rats and controls. The effect of dietary zinc content on isoproterenol-stimulated adenylate cyclase was significant in control rats only. Thus zinc intake appears to be an important determinant of cardiac adenylate cyclase activity level. Additional factors peculiar to the diabetic state are involved in the modulation of β-adrenergic responsiveness of the diabetic heart.

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